Influence of β<sub>2</sub>-Integrin Adhesion Molecule Expression and Pulmonary Infection with<i>Pasteurella haemolytica</i>on Cytokine Gene Expression in Cattle

Lee, Haa-Yung; Kehrli, Marcus E.; Brogden, Kim A.; Gallup, Jack M.; Ackermann, Mark R.

doi:10.1128/iai.68.7.4274-4281.2000

Cited by 15 publications

(11 citation statements)

References 42 publications

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“…Similar responses were observed for cytokine-treated peripheral blood mononuclear cells (PBMCs) subsequently exposed to leukotoxin (Leite et al, 2004a,b). These observations are supported by the report of Lee et al (2000), who provide in vivo evidence that ␤ 2 integrins are important in the pulmonary inflammatory response to M. haemolytica infection in cattle.…”

supporting

confidence: 76%

Complexities of the pathogenesis ofMannheimia haemolyticaandHaemophilus somnusinfections: challenges and potential opportunities for prevention?

Czuprynski

Leite

Sylte

et al. 2004

Anim. Health. Res. Rev.

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Progress in producing improved vaccines against bacterial diseases of cattle is limited by an incomplete understanding of the pathogenesis of these agents. Our group has been involved in investigations of two members of the family Pasteurellaceae, Mannheimia haemolytica and Haemophilus somnus, which illustrate some of the complexities that must be confronted. Susceptibility to M. haemolytica is greatly increased during active viral respiratory infection, resulting in rapid onset of a severe and even lethal pleuropneumonia. Despite years of investigation, understanding of the mechanisms underlying this viral-bacterial synergism is incomplete. We have investigated the hypothesis that active viral infection increases the susceptibility of bovine leukocytes to the M. haemolytica leukotoxin by increasing the expression of or activating the beta2 integrin CD11a/CD18 (LFA-1) on the leukocyte surface. In vitro exposure to proinflammatory cytokines (i.e. interleukin-1beta, tumor necrosis factor-alpha and interferon-gamma) increases LFA-1 expression on bovine leukocytes, which in turn correlates with increased binding and responsiveness to the leukotoxin. Alveolar macrophages and peripheral blood leukocytes from cattle with active bovine herpesvirus-1 (BVH-1) infection are more susceptible to the lethal effects of the leukotoxin ex vivo than leukocytes from uninfected cattle. Likewise, in vitro incubation of bovine leukocytes with bovine herpesvirus 1 (BHV-1) potentiates LFA-1 expression and makes the cells more responsive to leukotoxin. A striking characteristic of H. somnus infection is its propensity to cause vasculitis. We have shown that H. somnus and its lipo-oligosaccharide (LOS) trigger caspase activation and apoptosis in bovine endothelial cells in vitro. This effect is associated with the production of reactive oxygen and nitrogen intermediates, and is amplified in the presence of platelets. The adverse effects of H. somnus LOS are mediated in part by activation of endothelial cell purinergic receptors such as P2X7. Further dissection of the pathways that lead to endothelial cell damage in response to H. somnus might help in the development of new preventive or therapeutic regimens. A more thorough understanding of M. haemolytica and H. somnus virulence factors and their interactions with the host might identify new targets for prevention of bovine respiratory disease.

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supporting

confidence: 76%

Complexities of the pathogenesis ofMannheimia haemolyticaandHaemophilus somnusinfections: challenges and potential opportunities for prevention?

Czuprynski

Leite

Sylte

et al. 2004

Anim. Health. Res. Rev.

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“…Activated leukocytes in turn secrete mediators that increase vascular permeability and tissue injury (Bielefeldt-Ohmann et al, 1991). The BBE cell represents both a primary site of viral infection (Forman et al, 1982), and a physical barrier against microbial pathogens (Galdiero et al, 2002;Malazdrewich et al, 2001;Lee et al, 2000). Adherence of the bacterial cells to respiratory epithelium is a crucial and early event in the pathogenesis of BRD (Auger et al, 2009;Kisiela and Czuprynski, 2009).…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica

N’jai

Rivera

Atapattu

et al. 2013

Veterinary Immunology and Immunopathology

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Bovine respiratory disease (BRD) often occurs when active respiratory virus infections (BHV-1, etc.) impair resistance to Mannheimia haemolytica infection in the lower respiratory tract. The interactions that occur when the respiratory epithelium encounters these viral and bacterial pathogens are poorly understood. We used Agilent bovine gene microarray chips containing 44,000 transcripts to elucidate bovine bronchial epithelial cell (BBEC) responses following in vitro exposure to BHV-1 alone, M. haemolytica alone, or both BHV-1 and M. haemolytica. Microarray analysis revealed differential regulation (>2-fold) of 978 transcripts by BHV-1 alone, 2040 transcripts by M. haemolytica alone, and 2189 genes by BHV-1 and M. haemolytica in combination. M. haemolytica treatment produced significantly greater inductions (>10-fold) of several inflammation associated genes, such as CXCL2, IL-6, IL-1α, e-selectin, and IL-8, than to BHV-1 alone. Functional analysis of the microarray data revealed a significant upregulation of genes involved in important biological processes such as inflammation (TNF-α, IL-8, Tlr-2, IL-1, CXCL2, CSF2), vascular functions (VEGF, EDN2) and leukocyte migration (ICAM1, IL-16) during a co-infection with BHV-1 and M. haemolytica compared to either pathogen alone. This study provides evidence to support that lung epithelial cells are a source of mediators that may promote inflammatory changes observed during bovine respiratory disease.

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“…Neutrophils express functional receptors for nicotine and cotinine (148, 157), as well as tobacco-derived aryl hydrocarbons (158). Neutrophils also express receptors for several endogenous immunomodulatory factors that have been shown to be dysregulated in tobacco smokers.…”

Section: Smoking and Neutrophil Functionmentioning

confidence: 99%

Neutrophils in Periodontal Inflammation

Scott

Krauss²

2011

Frontiers of Oral Biology

208

216

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Influence of β₂-Integrin Adhesion Molecule Expression and Pulmonary Infection withPasteurella haemolyticaon Cytokine Gene Expression in Cattle

Abstract: ؊ cattle at 2 h p.i. was significantly higher than that of CD18 ؉ cattle; at 4 h p.i., there was no difference between the two groups. These data suggest that ␤ 2 -integrins may contribute to the induction of expression of some PIC genes, as a consequence of P. haemolytica infection.

Cited by 15 publications

References 42 publications

Complexities of the pathogenesis ofMannheimia haemolyticaandHaemophilus somnusinfections: challenges and potential opportunities for prevention?

Complexities of the pathogenesis ofMannheimia haemolyticaandHaemophilus somnusinfections: challenges and potential opportunities for prevention?

Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica

Neutrophils in Periodontal Inflammation

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Influence of β2-Integrin Adhesion Molecule Expression and Pulmonary Infection withPasteurella haemolyticaon Cytokine Gene Expression in Cattle

Abstract: ؊ cattle at 2 h p.i. was significantly higher than that of CD18 ؉ cattle; at 4 h p.i., there was no difference between the two groups. These data suggest that ␤ 2 -integrins may contribute to the induction of expression of some PIC genes, as a consequence of P. haemolytica infection.

Cited by 15 publications

References 42 publications

Complexities of the pathogenesis ofMannheimia haemolyticaandHaemophilus somnusinfections: challenges and potential opportunities for prevention?

Complexities of the pathogenesis ofMannheimia haemolyticaandHaemophilus somnusinfections: challenges and potential opportunities for prevention?

Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica

Neutrophils in Periodontal Inflammation

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Influence of β₂-Integrin Adhesion Molecule Expression and Pulmonary Infection withPasteurella haemolyticaon Cytokine Gene Expression in Cattle