Influences of acidic reaction and hydrolytic conditions on monosaccharide composition analysis of acidic, neutral and basic polysaccharides

Wang, Qingchi; Zhao, Xia; Pu, Jianghua; Luan, Xiao-Hong

doi:10.1016/j.carbpol.2016.02.023

Cited by 83 publications

(22 citation statements)

References 13 publications

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“…The results showed that levoglucosan, mannosan and galactosan were completely converted to glucose, mannose and galactose, respectively, while minimal loss was observed for neutral sugars and alditols (Fig. S 2), which is consistent with previous studies (Skoog and Benner, 1997;Wang et al, 2016).…”

Section: Marine Pomsupporting

confidence: 91%

Simultaneous determination of neutral sugars, alditols and anhydrosugars using anion-exchange chromatography with pulsed amperometric detection: Application for marine and atmospheric samples

2019

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An improved high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) method is described for the simultaneous determination of neutral sugars (hexoses, pentoses and deoxysugars), alditols and anhydrosugars commonly found in atmospheric and marine samples. The method uses a CarboPac MA1 column, at a flow rate of 0.3 mL min-1 , and a NaOH gradient (250-700 mM). The proposed method applies a temperature gradient (from 25 to 28 °C) to the column for the first 30 min of the analysis, followed by a constant temperature at 28 °C until the end of the analysis. These analytical conditions allowed the separation of 15 out of 17 carbohydrates in 75 min with resolution factors better than 0.5 for the critical pairs levoglucosan/arabitol, galactosan/arabinose, arabinose/mannose and glucose/xylose. The application of this method to field samples revealed that anhydrosugars represented 53% of total neutral carbohydrates (TCHO) in total suspended atmospheric particles (TSP), whereas they were detected for the first time in marine particulate organic matter (POM) and high-molecular-weight dissolved organic matter (HMWDOM) samples accounting 2% and 3% of TCHO, respectively. Levoglucosan and/or galactosan were the major anhydrosugars in all samples however, their concentrations are undoubtedly underestimated because hydrolysis was applied to the marine samples prior to the HPAEC-PAD analysis. Despite this underestimation, their presence in the marine samples clearly indicates possible terrestrial input most likely via atmospheric deposition as these compounds are considered terrestrial burring biomass tracers. Finally, deoxysugars were also detected for the first time in the TSP sample representing 1% of TCHO, while alditols accounted for 0.4% and 0.3% of TCHO in POM and HMWDOM, respectively.

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Section: Marine Pomsupporting

confidence: 91%

Simultaneous determination of neutral sugars, alditols and anhydrosugars using anion-exchange chromatography with pulsed amperometric detection: Application for marine and atmospheric samples

2019

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“…The monosaccharide composition of polysaccharide played a critical role in the biological activity of polysaccharide [28]. HPLC was applied to determine the monosaccharide composition of DOP-40, DOP-50, DOP-60, and DOP-70, and the results indicated that four fractions mainly consisted of d -mannose and d -glucose in different molar ratios.…”

Section: Discussionmentioning

confidence: 99%

Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

Xing

Zhang

et al. 2018

Chemistry Central Journal

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BackgroundDendrobium officinale as a precious traditional Chinese herb is widely used in medicines and health supplements. Thus the extraction, purification and biological activities of polysaccharides from the stem of Dendrobium officinale have significant meaning on theory and application value.MethodsThe crude Dendrobium officinale polysaccharide (DOP) was obtained by hot water extraction- ethanol precipitation method, and four new polysaccharide fractions (DOP-40, DOP-50, DOP-60, and DOP-70) were further obtained from the crude DOP by fractional precipitation with ethanol method, then four fractions were further purified by Toyopearl-H65F gel resin. The molecular weight and monosaccharide composition of four purified fractions were determined by high performance anion exchange chromatography and high performance liquid chromatography. The antioxidant activities of them were evaluated by the reducing power assay, and the superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and hydroxyl free radicals scavenging assays, respectively. Finally, the anticancer activities of them were investigated via the MTT assay and the western blot analysis using HepG2 cells.ResultsAmong these four purified fractions were mainly composed of d-mannose and d-glucose with different molar ratios, and their average molecular weights were 999, 657, 243 and 50.3 kDa, respectively. What’s more, DOP-70 always exhibited the strongest antioxidant and anticancer activities, while DOP-40 and DOP-60 showed very close antioxidant and anticancer activities which were better than that of DOP-50. The western blotting analysis also showed that DOP-40, DOP-60, and DOP-70 induced apoptosis in HepG2 human liver cancer cells through the Bcl-2 and Bax-dependent pathway.ConclusionsFractional precipitation with ethanol could successfully apply to extract four new polysaccharide fractions from Dendrobium officinale stems, and the polysaccharide fractions possessed efficient antioxidant and anticancer activities, especially DOP-70.

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“…Orthogonal experiment is a common and simple method to study multiple factors and multiple levels, which can optimize the process by analyzing the typical experimental results [22,29,51,52,53]. In order to investigate optimal hydrolysis conditions of PPS, the orthogonal L 9 (3) 4 experiments were designed.…”

Section: Methodsmentioning

confidence: 99%

“…A great number of analytical techniques have been used to detect monosaccharides in complex samples, including thin-layer chromatography (TLC) [13], gas chromatography (GC) [14], high-performance liquid chromatography (HPLC) [15,16,17,18,19], capillary electrophoresis (CE) [20,21,22], gas chromatography–mass spectrometry(GC–MS) [23,24], and liquid chromatography–mass spectrometry (LC–MS) [25,26,27]. HPLC has been accepted as one of the common techniques for the analysis of sugars, which was used for simultaneous determination of neutral, acidic, and basic carbohydrates [28,29]. However, the lack of chromophores or fluorophores in the structure of monosaccharides prevents direct detection by UV or fluorescence detectors.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS

et al. 2019

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Polyporus umbellatus is a well-known and important medicinal fungus in Asia. Its polysaccharides possess interesting bioactivities such as antitumor, antioxidant, hepatoprotective and immunomodulatory effects. A qualitative and quantitative method has been established for the analysis of 12 monosaccharides comprising polysaccharides of Polyporus umbellatus based on high-performance liquid chromatography coupled with electrospray ionization–ion trap–time of flight–mass spectrometry. The hydrolysis conditions of the polysaccharides were optimized by orthogonal design. The results of optimized hydrolysis were as follows: neutral sugars and uronic acids 4 mol/L trifluoroacetic acid (TFA), 6 h, 120 °C; and amino sugars 3 mol/L TFA, 3 h, 100 °C. The resulting monosaccharides derivatized with 1-phenyl-3-methyl-5-pyrazolone have been well separated and analyzed by the established method. Identification of the monosaccharides was carried out by analyzing the mass spectral behaviors and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone labeled monosaccharides. The results showed that polysaccharides in Polyporus umbellatus were composed of mannose, glucosamine, rhamnose, ribose, lyxose, erythrose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, and fucose. Quantitative recoveries of these monosaccharides in the samples were in the range of 96.10–103.70%. This method is simple, accurate, and sensitive for the identification and quantification of monosaccharides, and can be applied to the quality control of Polyporusumbellatus as a natural medicine.

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Influences of acidic reaction and hydrolytic conditions on monosaccharide composition analysis of acidic, neutral and basic polysaccharides

Cited by 83 publications

References 13 publications

Simultaneous determination of neutral sugars, alditols and anhydrosugars using anion-exchange chromatography with pulsed amperometric detection: Application for marine and atmospheric samples

Simultaneous determination of neutral sugars, alditols and anhydrosugars using anion-exchange chromatography with pulsed amperometric detection: Application for marine and atmospheric samples

Physicochemical properties of polysaccharides from Dendrobium officinale by fractional precipitation and their preliminary antioxidant and anti-HepG2 cells activities in vitro

Quantitative Analysis of Polysaccharide Composition in Polyporus umbellatus by HPLC–ESI–TOF–MS

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