Influences of food matrices on cytotoxicity of asiatic acid in mammalian cell models

Abstract

This study investigated the cytotoxicity of asiatic acid (AA) encapsulated in different protein-carbohydrate matrices in human colon adenocarcinoma SW620, human hepatoblastoma HepG2 and diploid monkey kidney cells (Vero). Mungbean protein concentrate (MPC) and mungbean protein hydrolysate (MPH) in lactose (L) or lactose-maltodextrin (LM) mixture, were used to fabricate matrices encapsulating AA during lyophilisation. The cytotoxicity of AA was in a dose-dependent manner for HepG2 and SW620 cells, but not for Vero cells. The 50% inhibitory concentrations (IC 50 ) of AA in HepG2 and SW620 were 120 and 130 lm respectively. The IC 50 of AA encapsulated in MPH-L matrix in HepG2 was reduced from that of AA alone to 80 lm (P < 0.05). Study of the anti-cancer activity of AA, in the absence and presence of food matrices, revealed that an MPH-L matrix led AA to cause more apoptosis deaths in HepG2 cells than either a MPC-L matrix or pure AA.Influences of food matrices on cytotoxicity of asiatic acid K. Jirasripongpun et al.Influences of food matrices on cytotoxicity of asiatic acid K. Jirasripongpun et al.

Influence of carbohydrates on self‐association of mung bean protein hydrolysate in the presence of amphiphilic asiatic acid

“…This study showed that antioxidative MPH from tryptic hydrolysis, which had a fairly high surface charge of (−)30 mV, could reduce micellisation of AA in the aqueous phase. The MPH–AA co‐aggregates may help to increase cellular uptake via passive diffusion by controlling the particle size to around 300 nm (Wongekalak et al ., ; Jirasripongpun et al ., ). The degree of co‐aggregation between MPH and AA was found to be dependent on their ratio and the type of carbohydrate in the bulk aqueous phase.…”

“…Our previous investigation indicated that different carbohydrate excipients used during drying of antioxidative mung bean protein hydrolysate (MPH)-asiatic acid (AA) resulted in different anticancer efficacy of AA in human hepatoblastoma HepG2 cells when MPH was used as a peptide carrier for AA. It was also found that the disaccharide lactose enhanced the cytotoxicity of MPH-AA co-aggregates compared with that of AA alone (Wongekalak et al, 2011;Jirasripongpun et al, 2012). The influence of different carbohydrates on the efficacy of MPH-AA for cellular uptake led to the suggestion that the molecular weight (MW) of the carbohydrate may influence the aggregation nature of AA and MPH-AA.…”

Antimicrobial Effect of Asiatic Acid Against Clostridium difficile Is Associated With Disruption of Membrane Permeability

“…Prior to the drug administration, cytotoxicity of the compounds against host cells is needed to be evaluated ( Tharmalingam et al, 2018 ). IC 50 values for AA were reported to be greater than 100 μM (∼50 μg/ml) on human umbilical vein endothelial cells and kidney epithelial Vero cells ( Jirasripongpun et al, 2012 ; Huang et al, 2016 ), as well as no significant effect of the compound at the same concentration was observed on viability of human gastric mucosa epithelial cells ( Jing et al, 2016 ). Additionally, up to 165 mg/kg of AA was administered to the mice without any signal of side effects ( Krishnamurthy et al, 2009 ).…”