Influences of sample storage and grinding on the extraction of soil amino sugars

Mou, Zhijian; Kuang, Luhui; Yan, Biyue; Zhang, Xinyu; Wang, Yunqiang; Liu, Zhanfeng

doi:10.1007/s42832-020-0031-9

Cited by 25 publications

(10 citation statements)

References 29 publications

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“…Soil amino sugars were extracted according to Indorf et al. (2011) with minor modifications (Mou et al., 2020). Briefly, 0.5 g of freeze‐dried soil was hydrolyzed with 10 ml of 6 M HCl at 105°C for 6 hr.…”

Section: Methodsmentioning

confidence: 99%

Phosphorus addition decreases microbial residual contribution to soil organic carbon pool in a tropical coastal forest

Yuan

Mou

et al. 2020

Global Change Biology

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109

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The soil nitrogen (N) and phosphorus (P) availability often constrains soil carbon (C) pool, and elevated N deposition could further intensify soil P limitation, which may affect soil C cycling in these N‐rich and P‐poor ecosystems. Soil microbial residues may not only affect soil organic carbon (SOC) pool but also impact SOC stability through soil aggregation. However, how soil nutrient availability and aggregate fractions affect microbial residues and the microbial residue contribution to SOC is still not well understood. We took advantage of a 10‐year field fertilization experiment to investigate the effects of nutrient additions, soil aggregate fractions, and their interactions on the concentrations of soil microbial residues and their contribution to SOC accumulation in a tropical coastal forest. We found that continuous P addition greatly decreased the concentrations of microbial residues and their contribution to SOC, whereas N addition had no significant effect. The P‐stimulated decreases in microbial residues and their contribution to SOC were presumably due to enhanced recycling of microbial residues via increased activity of residue‐decomposing enzymes. The interactive effects between soil aggregate fraction and nutrient addition were not significant, suggesting a weak role of physical protection by soil aggregates in mediating microbial responses to altered soil nutrient availability. Our data suggest that the mechanisms driving microbial residue responses to increased N and P availability might be different, and the P‐induced decrease in the contribution of microbial residues might be unfavorable for the stability of SOC in N‐rich and P‐poor tropical forests. Such information is critical for understanding the role of tropical forests in the global carbon cycle.

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Section: Methodsmentioning

confidence: 99%

Phosphorus addition decreases microbial residual contribution to soil organic carbon pool in a tropical coastal forest

Yuan

Mou

et al. 2020

Global Change Biology

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109

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“…Among them, MurA exclusively exists in bacteria, GluN exists in both fungi and bacteria (Amelung et al, 2001), and GalN generally exists in bacteria and the cell walls of some rare archaeal species (Joergensen, 2018). These three amino sugars were quanti ed according to the method described by Indorf et al (2011) with minor modi cations (Mou et al, 2020). The chromatograms of standard solutions containing mixed amino sugars were applied to identify and quantify each amino sugar.…”

Section: Laboratory Analysismentioning

confidence: 99%

The vertical distribution pattern of microbial- and plant-derived carbon in the rhizosphere in alpine coniferous forests

et al. 2021

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Background and aimsWhile the quantitative assessment of plant-and microbial-derived carbon (C) in the soil organic C (SOC) chemical composition in soil pro les has been initially explored, the vertical distribution pattern of these two C sources and their dominant role in SOC formation based on the insights related to the rhizosphere are still lacking. MethodsWe quanti ed the divergent accumulation of microbial-derived C (i.e., microbial residues), plant-derived C (i.e., lipids and lignin phenols) and SOC in the rhizosphere at various depths (0-10 cm, 10-20 cm and 20-30 cm) in the upper mineral soil and analyzed its control factors in an alpine coniferous forest (Picea asperata. Mast). We further revealed the relative contribution of plant-or microbial-derived C to rhizosphere SOC in the soil pro le. ResultsThe contents of microbial-and plant-derived C and SOC in the rhizosphere decreased with soil depth and were mainly regulated by root and microbial biomass. Moreover, the contribution of microbial-derived C dominated by fungal residues to rhizosphere SOC at each soil depth (more than 62%) was much higher than that of plant-derived C (less than 6%), implying that the soil microbial C pump was intensely stimulated in the rhizosphere. ConclusionsThese results indicated that microbial-derived C was the main contributor of rhizosphere SOC at various depths in the upper mineral soil. Our ndings provide direct experimental evidence for assessing the dominant contribution of microbial-or plant-derived C to SOC in the soil pro le from the perspective of the rhizosphere.

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“…Amino sugars (ASs), including muramic acid (MurN), galactosamine (GalN), and glucosamine (GlcN), were measured as described in Indorf et al (2011), with minor modification (Mou et al, 2020). In brief, ASs were hydrolyzed, extracted, and derivatized with ortho‐phthaldialdehyde, determined by high‐performance liquid chromatography (Dionex Ultimate 3000, Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

Faster accumulation and greater contribution of glomalin to the soil organic carbon pool than amino sugars do under tropical coastal forest restoration

Yuan

Mou

et al. 2022

Global Change Biology

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Microbial metabolic products play a vital role in maintaining ecosystem multifunctionality, such as soil physical structure and soil organic carbon (SOC) preservation.Afforestation is an effective strategy to restore degraded land. Glomalin-related soil proteins (GRSP) and amino sugars are regarded as stable microbial-derived C, and their distribution within soil aggregates affects soil structure stability and SOC sequestration. However, the information about how afforestation affects the microbial contribution to SOC pools within aggregates is poorly understood. We assessed the accumulation and contribution of GRSP and amino sugars within soil aggregates along a restoration chronosequence (Bare land, Eucalyptus exserta plantation, native species mixed forest, and native forest) in tropical coastal terraces. Amino sugars and GRSP concentrations increased, whereas their contributions to the SOC pool decreased along the restoration chronosequence. Although microaggregates harbored greater microbial abundances, amino sugars and GRSP concentrations were not significantly affected by aggregate sizes. Interestingly, the contributions of amino sugars and GRSP to SOC pools decreased with decreasing aggregate size which might be associated with increased accumulation of plant-derived C. However, the relative change rate of GRSP was consistently greater in all restoration chronosequences than that of amino sugars. The accumulation of GRSP and amino sugars in SOC pools was closely associated with the dynamics of soil fertility and the microbial community. Our findings

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Influences of sample storage and grinding on the extraction of soil amino sugars

Cited by 25 publications

References 29 publications

Phosphorus addition decreases microbial residual contribution to soil organic carbon pool in a tropical coastal forest

Phosphorus addition decreases microbial residual contribution to soil organic carbon pool in a tropical coastal forest

The vertical distribution pattern of microbial- and plant-derived carbon in the rhizosphere in alpine coniferous forests

Faster accumulation and greater contribution of glomalin to the soil organic carbon pool than amino sugars do under tropical coastal forest restoration

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