“…Theoretically, 2.86 mg of COD are required to reduce 1 mg of nitrate nitrogen, approximately 2 mg/ L COD of carbon source are required for removing nitrogen through denitrification, and overall, approximately 11 mg/L COD of carbon source are consumed in the anaerobic stage. Research has shown that in the anaerobic stage, phosphorus-accumulating bacteria firstly utilize quality carbon sources, such as volatile fatty acids (VFA) and polyhydroxybutyric acid, and degrade intracellular polymeric phosphate and glycogen to increase soluble phosphorus concentrations (Xiong et al, 2012). Thus, in the anaerobic stage, RBCOD was entirely used to release phosphorus, 3.15 mg/L of SBCOD were used to release phosphorus, and all carbon sources required for nitrogen removal by denitrification were obtained from SBCOD.…”