Influência da ciprofloxacina na consolidação óssea de fraturas de fêmur em ratos

Kupczik, Fabiano; Vialle, Luiz Roberto; Nobre, Leonardo Oliveira; Vieira, Lucas de Almeida; Fernandes, André Eugênio Omine

doi:10.1590/s1413-78522009000400007

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…5 ), compared to other groups suggesting more mature consolidation and increased resistance of bone tissue and confirming the radiographic results. Biomechanical assays should only be performed after 60 days in view of the evolution of bone callus formation, as reported by Peng et al [ 29 ] using a paste of herbs for fracture treatment in rabbits [ 30 ] and by Kupczik et al [ 34 ] using ciprofloxacin for bone consolidation of rat femoral fractures [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

Neto

Ribeiro

Morais

et al. 2017

BMC Complement Altern Med

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BackgroundBone defects caused by trauma, infection or tumor resection are common in orthopedic clinic and depending on the extent of the fracture; the vast majority require treatment with bone substitutes. Among the bone grafts employees, the autograft is defined as the gold standard, but with some limitations, principally related to morbidity at the site of its removal, with the need to search for other biomaterials as adjuvant in bone regeneration. Therefore, the objective of the present study was to evaluate the use of Chenopodium ambrosioides as a bone graft substitute for the osseointegration of fractures in rabbits, compared to other bone grafts already employed in the surgical routine as Ricinus communis (castor oil) polyurethane and autogenous bone marrow.MethodsForty-eight rabbits were divided into four groups. After anesthesia, a radius fracture was created and the animals were treated with the following grafts: C. ambrosioides graft, autogenous bone marrow, castor oil and saline (control). After 30, 60 and 90 days, the animals were submitted to radiographical and histological analyses and bone alkaline phosphatase, osteocalcin, biomechanical tension, and collagen were measured. We also realized a phytochemical screening and in vitro antioxidant activity including 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical.ResultsThe data showed that the growth of the bone callus was more expressive and biomechanical assessment showed better tensile strength in C. ambrosioides graft. The experimental results also revealed that there was significantly greater activity of the bone alkaline phosphatase and osteocalcin during early fracture healing, similarly to the group receiving autogenous bone marrow. Histologically, immature bone was observed in C. ambrosioides graft at 30 days, whereas the formation of cartilaginous tissue predominated in the other groups. A higher amount of type I collagen was observed in C. ambrosioides graft throughout treatment. It was detected strong presence of flavonoids and appreciable antioxidant activity.ConclusionsThe results indicate that C. ambrosioides graft and autogenous bone marrow have similar ability to enhance bone regeneration, higher than the castor oil graft, suggesting that the medicinal plant can provide therapeutic benefits for bone tissue.

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Section: Discussionmentioning

confidence: 99%

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

Neto

Ribeiro

Morais

et al. 2017

BMC Complement Altern Med

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“…It is commonly administrated for P. aeruginosa osteomyelitis and is widely used as a prophylactic measure in osteomyelitis surgeries [ 5 – 7 ]. The drug shows efficacy and safety in the treatment of adult patients with serious skin and soft tissue infections caused by a variety of bacterial pathogens [ 8 ]. The spectrum of activity of the CIP and the location of several injuries which can be treated with this drug open discussion for a new site-specific approach for some infirmities.…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin Release Using Natural Rubber Latex Membranes as Carrier

Murbach

Ogawa

Borges

et al. 2014

International Journal of Biomaterials

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Natural rubber latex (NRL) from Hevea brasiliensis is easily manipulated, low cost, is of can stimulate natural angiogenesis and cellular adhesion, is a biocompatible, material and presents high mechanical resistance. Ciprofloxacin (CIP) is a synthetic antibiotic (fluoroquinolone) used in the treatment of infection at external fixation screws sites and remote infections, and this use is increasingly frequent in medical practice. The aim of this study was to develop a novel sustained delivery system for CIP based on NRL membranes and to study its delivery system behavior. CIP was found to be adsorbed on the NRL membrane, according to results of energy dispersive X-ray spectroscopy. Results show that the membrane can release CIP for up to 59.08% in 312 hours and the mechanism is due to super case II (non-Fickian). The kinetics of the drug release could be fitted with double exponential function X-ray diffraction and Fourier transform infrared (FTIR) spectroscopy shows some interaction by hydrogen bound, which influences its mechanical behavior.

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“…Similar findings were described in a study by Kupczik et al . 24 , who showed that high doses of ciprofloxacin could interfere with matrix formation with no difference in callus mechanical resistance, and supported by Miclau et al . 30 , that demonstrated decrease of osteoblasts number in a dose dependent manner when the osteoblasts were exposed to high concentration of ciprofloxacin.…”

Section: Discussionmentioning

confidence: 77%

Prevention of bone infection after open fracture using a chitosan with ciprofloxacin implant in animal model

et al. 2020

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To evaluate different concentrations of ciprofloxacin to prevent infection after open fracture contaminated with S. aureus in rats using absorbable local delivery system. Methods: Fifty-two Wistar rats were assigned to six groups. After 4 weeks, all animals underwent 99mTc-ceftizoxima scintigraphy evaluation, callus formation measurement and histological analysis. ANOVA, t-Student and Kruskal Wallis were used for quantitative variables statistical analysis, whereas qui square and exact Fisher were used for qualitative variables. Results: Treatment using 25% and 50% of ciprofloxacin incorporated at the fracture fixation device were effective in preventing bone infection compared to control group (p<0.05). Chitosan were not effective in preventing bone infection when used alone compared to control group (p>0.05). Histological findings demonstrated bone-healing delay with 50% of ciprofloxacin. No difference in callus formation were observed (p>0.05). Conclusion: Local delivery treatment for contaminated open fracture using chitosan with ciprofloxacin is effective above 25%.

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Influência da ciprofloxacina na consolidação óssea de fraturas de fêmur em ratos

Cited by 8 publications

References 23 publications

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

Chenopodium ambrosioides as a bone graft substitute in rabbits radius fracture

Ciprofloxacin Release Using Natural Rubber Latex Membranes as Carrier

Prevention of bone infection after open fracture using a chitosan with ciprofloxacin implant in animal model

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