Influência da glutamina na mucosa do instestino de ratos submetidos à enterectomia extensa

Abstract

OBJETIVO: Avaliar a influência de uma dieta suplementada com glutamina sobre as alterações adaptativas no intestino delgado de ratos com enterectomia extensa. MÉTODO: Vinte ratos Wistar, divididos aleatoriamente em dois grupos de dez animais, foram enterectomizados e alimentados com dois tipos diferentes de dieta nos 14 dias de pós-operatório: grupo controle (GC)-dieta padrão; grupo glutamina (GG)-dietapadrão acrescida de 3,05% de glutamina. Avaliou-se evolução ponderal, peso da mucosa intestinal (PM), profundidade das criptas (PC), altura das vilosidades (AV), espessura da parede (EP) e o conteúdo de ácido desoxirribonucléico (DNA) na mucosa intestinal, no início e no final do experimento. RESULTADOS: Com exceção da PC ileal do Grupo GG, todas as variáveis estudadas tiveram um aumento significativo em seus valores finais tanto no jejuno quanto no íleo (p<0,05).Entre os grupos, a comparação do PM, AV, DNA da mucosa, no jejuno e no íleo, tanto inicialmente quanto no final do estudo, bem como da EP inicial no jejuno e íleo eda PC no jejuno final e no íleo inicial e final não mostraram diferenças significativas (p>0,05). No jejuno inicial, a PC no grupo GC foi maior (p=0,005). A EP do jejuno e íleo final foi maior no grupo GC. CONCLUSÃO: A suplementação dietética com a glutamina não melhorou as alterações adaptativas que ocorrem no remanescente intestinal.

Weight loss and morphometric study of intestinal mucosa in rats after massive intestinal resection: influence of a glutamine-enriched diet

“…9 Glutamine supplementation has been used in clinical and experimental situations of trophic reduction and intestinal absorption damage, although its real efficiency has not yet been proved. [20][21][22] When associated with growth hormone, glutamine supplementation has been shown to have a gut-trophic effect and to enhance intestinal absorption, both experimentally 4,22 and in clinical studies. 17 However, other researchers have not found considerable improvement with the association of glutamine and growth hormone.…”

“…All animals underwent an extensive small bowel resection, including the ileocecal valve, leaving a remnant jejunum of only 25 cm from the pylorus that was anastomosed lateral-laterally to the ascendant colon. The animals were weighed at the beginning and end of the experiment (20 th postoperative day). Then they were killed and the remnant intestine was removed.…”

Effects of the combined use of glutamine and growth hormone in the intestinal adaptation after massive resection of the small bowel in rats

“…Experimental and clinical studies have demonstrated the possible benefits with the use of this association in the treatment of intestinal insufficiency, after extensive resection of the small bowel [13][14][15][16][17][18][19][20] . Iglesias and Zucoloto 27 had indicated that the adaptative intestinal reactions initiate in the first few days after enterectomy.…”

“…There are evidences that glutamine (GL) is the principal fuel for enterocytes [11][12][13] . Curiously, some experimental works 14,15 using diets rich in GL have not achieved good results in experimental SBS. However, the combined use of GL and GH may induce important trophic effects on the intestinal mucosa.…”