Influência da suplementação aguda e crônica de creatina sobre as concentrações sanguíneas de glicose e lactato de ratos Wistar

Souza, Renato Aparecido de; Santos, Roberto Mussoline dos; Osório, Rodrigo Alexis Lazo; Cogo, José Carlos; Júnior, Antônio Carlos Guimarães Prianti; Lopes-Martins, Rodrigo Álvaro Brandão; Ribeiro, Wellington

doi:10.1590/s1517-86922006000600012

Cited by 10 publications

(15 citation statements)

References 25 publications

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“…Taken in aggregate, these results for pro-oxidant markers underscore the findings of Sjodin et al [36] and Souza et al [37], that is, predominantly aerobic exercise causes increased oxygen flow in the mitochondria and approximately five percent of this oxygen is not completely reduced, thereby forming ROS.…”

Section: Discussionsupporting

confidence: 83%

Creatine supplementation and oxidative stress in rat liver

Araújo

Moura

Vieira

et al. 2013

Journal of the International Society of Sports Nutrition

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BackgroundThe objective of this study was to determine the effects of creatine supplementation on liver biomarkers of oxidative stress in exercise-trained rats.MethodsForty 90-day-old adult male Wistar rats were assigned to four groups for the eight-week experiment. Control group (C) rats received a balanced control diet; creatine control group (CCr) rats received a balanced diet supplemented with 2% creatine; trained group (T) rats received a balanced diet and intense exercise training equivalent to the maximal lactate steady state phase; and supplemented-trained (TCr) rats were given a balanced diet supplemented with 2% creatine and subjected to intense exercise training equivalent to the maximal lactate steady state phase. At the end of the experimental period, concentrations of creatine, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) were measured as well as the enzyme activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-GPx) and catalase (CAT). Liver tissue levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio were also determined.ResultsHepatic creatine levels were highest in the CCr and TCr groups with increased concentration of H2O2 observed in the T and TCr animal groups. SOD activity was decreased in the TCr group. GSH-GPx activity was increased in the T and TCr groups while CAT was elevated in the CCr and TCr groups. GSH, GGS and the GSH/GSSG ratio did not differ between all animal subsets.ConclusionsOur results demonstrate that creatine supplementation acts in an additive manner to physical training to raise antioxidant enzymes in rat liver. However, because markers of liver oxidative stress were unchanged, this finding may also indicate that training-induced oxidative stress cannot be ameliorated by creatine supplementation.

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Section: Discussionsupporting

confidence: 83%

Creatine supplementation and oxidative stress in rat liver

Araújo

Moura

Vieira

et al. 2013

Journal of the International Society of Sports Nutrition

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“…The animals in the TCr and T groups showed decreased body mass relative to the animals of the C and CCr groups. Creatine supplementation has been shown to produce contradictory effects on the change in body mass, as some authors have demonstrated an increase in body mass (Volek et al, 2004;Souza et al, 2006) and others have observed no change in body mass (Louis et al, 2003;Franco et al, 2007). Therefore, our hypothesis was that the observed body weight loss of the animals was not due to creatine supplementation but was solely a result of exercise.…”

Section: Discussionmentioning

confidence: 89%

“…Some authors have reported that creatine supplementation may modify the use of energetic substrates, such as glucose and lactate, and possibly improve physical performance during extended exercises that preferentially use aerobic metabolism (Bembem et al, 2005;Souza et al 2006;Khanna & Manna, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The metabolic profile and energy homeostasis of muscle cells vary with changes in their activity level and supply of energy substrates. Previous studies have reported increased rates of insulin secretion (Souza et al, 2006), increased expression of GLUT-4 receptors (Op'Teijnde et al, 2001;Souza et al, 2006) and increased intramuscular glycogen concentration after supplementation with creatine (Young et al, 2002). Although it is believed that creatine has a hypoglycemic effect, several authors failed to confirm hyperinsulinemia after the ingestion of creatine (Newman et al, 2003;Freire et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Influence of creatine supplementation on indicators of glucose metabolism in skeletal muscle of exercised rats

Araújo

Vieira

Moura

et al. 2013

Motriz: rev. educ. fis.

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Abstract-The purpose of this study was to evaluate the effect of creatine supplementation in the diet on indicators of glucose metabolism in skeletal muscle of exercised rats. Forty Wistar adult rats were distributed into four groups for eight weeks: 1) Control: sedentary rats that received balanced diet; 2) Creatine control: sedentary rats that received supplementation of 2% creatine in the balanced diet; 3) Trained: rats that ran on a treadmill at the Maximal Lactate Steady State and received balanced diet; and 4) Supplemented-trained: rats that ran on a treadmill at the Maximal Lactate Steady State and received creatine supplementation (2%) in the balanced diet. The hydric intake increased and the body weight gain decreased in the supplemented-trained group. In the soleus muscle, the glucose oxidation increased in both supplemented groups. The production of lactate and glycemia during glucose tolerance test decreased in the supplemented-trained group. Creatine supplementation in conjunction with exercise training improved muscular glycidic metabolism of rats.Keywords: somatic index, glucose tolerance, physical activity Resumo-"Influência da suplementação com creatina em indicadores de metabolismo da glucose no músculo esquelético de ratos exercitados." O objetivo deste estudo foi avaliar o efeito da suplementação de creatina na dieta sobre indicadores do metabolismo glicídico musculoesquelético de ratos exercitados. Quarenta ratos Wistar adultos foram divididos em quatro grupos por oito semanas: Controle: receberam dieta balanceada, mantidos sedentários; Controle Creatina: receberam suplementação de creatina (2%) na dieta balanceada, mantidos sedentários; Treinado: correram em esteira na intensidade da máxima fase estável de lactato e receberam a dieta balanceada e grupo Treinado Suplementado: correram em esteira na intensidade da máxima fase estável de lactato e receberam suplementação de creatina (2%) na dieta balanceada. A ingestão hídrica aumentou e o ganho de massa corporal reduziu no grupo treinado e suplementado. No músculo sóleo, a oxidação de glicose aumentou em ambos os grupos suplementados. A produção de lactato e a glicemia durante teste de tolerância à glicose diminuíram no grupo treinado e suplementado. A suplementação com creatina em conjunto com treinamento físico melhorou metabolismo de glicídico muscular dos ratos.Palavras-chaves: índice somático, tolerância à glicose, atividade físicaResumen-"Influencia de la suplementación con creatina sobre indicadores del metabolismo de la glucosa en el músculo esquelético de ratas ejercitadas." El objetivo de este estudio fue evaluar el efecto de la suplementación con creatina en la dieta sobre indicadores del metabolismo de glucosa en el músculo esquelético en ratones ejercitados. Cuarenta ratas macho adultas Wistar se dividieron en cuatro grupos de ocho semanas: Control: recibieron dieta equilibrada, mantenido sedentaria; Control Complementado: La suplementación con creatina recibido (2%) en la dieta equilibrada, sedentaria mantenido; Trained: corrien...

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“…In a study involving adult male Wistar rats submitted to low-intensity and exhaustive swimming exercise, lactate concentrations were found to be lower in trained animals than in sedentary animals 16 . After 8 weeks of swimming exercise at 80% of maximum load, a reduction in blood lactate concentration was identified when compared to the group of sedentary animals 17 . The divergences between these studies and the present study are probably due to differences in the experimental protocols.…”

Section: Discussionmentioning

confidence: 97%

Efeito de consumo de carboidrato e de exercício físico sobre a concentração de glicogênio

Leite

Rombaldi

2014

Rev. Bras. Cineantropom. Desempenho Hum.

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-Carbohydrate is an important source of energy which is stored as glycogen. Since this storage may be related to the use of sports drinks, this study evaluated the changes in liver, skeletal muscle and kidney glycogen content of rats supplemented with maltodextrin and submitted to aerobic exercise at maximum lactate steady state or highintensity anaerobic exercise. Sixty-nine male Wistar rats, 60 days old at the beginning of the experiment, were used. The training protocol consisted of 8 weeks of continuous aerobic (60 min/day) or intermittent swimming (two periods of 30 min/day at an interval of 10 min and an exercise/rest ratio of 15 seconds), with overloads corresponding to 5% and 10% of body weight, respectively. The animals were supplemented for 37 days with a daily dose of 0.48 g/kg maltodextrin dissolved in water or received pure water. Factorial ANOVA or the Kruskal-Wallis test was used for data analysis. Aerobic training resulted in a significant increase in blood glucose (p˂0.02) and in a decline in hepatic glycogen content (p<0.02), while anaerobic exercise promoted a significant increase in lactate concentration (p<0.001) and a decrease in skeletal muscle (p=0.02) and kidney (p<0.03) glycogen content. Maltodextrin supplementation significantly increased muscle (p=0.008) and kidney (p<0.02) glycogen content in rats submitted to aerobic exercise. Eight weeks of aerobic and anaerobic exercise caused changes in liver, skeletal muscle and kidney glycogen content, as well as in blood glucose and blood lactate. Maltodextrin supplementation was effective in increasing skeletal muscle and kidney glycogen stores in rats submitted to aerobic exercise.

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Influência da suplementação aguda e crônica de creatina sobre as concentrações sanguíneas de glicose e lactato de ratos Wistar

Cited by 10 publications

References 25 publications

Creatine supplementation and oxidative stress in rat liver

Creatine supplementation and oxidative stress in rat liver

Influence of creatine supplementation on indicators of glucose metabolism in skeletal muscle of exercised rats

Efeito de consumo de carboidrato e de exercício físico sobre a concentração de glicogênio

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