Influencia de la enfermedad pulmonar obstructiva crónica en los componentes orgánicos volátiles en pacientes con cáncer de pulmón no microcítico

Muñoz-Lucas, M. Ángeles; Jareño-Esteban, Javier; Gutiérrez-Ortega, Carlos; López-Guijarro, Pablo; Collado-Yurrita, Luis; Quintana-Díaz, Manuel; Callol-Sánchez, Luis Miguel

doi:10.1016/j.arbres.2019.12.023

Cited by 7 publications

(1 citation statement)

References 28 publications

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“…In an alveolus, the majority of surface area (>95%) is covered by large and thin type-I pneumocytes [2]. Since the lung directly faces ambient environments, exposure to hazardous atmospheres like air pollution and volatile organic compounds (VOCs) can cause damage to the pneumocytes [3][4][5][6][7]. In addition, pathological conditions such as chronic obstruction pulmonary disease (COPD) and, recently, COVID-19 pneumonia may also impair proper functions of the pneumocytes.…”

Section: Introductionmentioning

confidence: 99%

Transwell Insert-Embedded Microfluidic Devices for Time-Lapse Monitoring of Alveolar Epithelium Barrier Function under Various Stimulations

Chang

Liao

et al. 2021

Micromachines

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This paper reports a transwell insert-embedded microfluidic device capable of culturing cells at an air-liquid interface (ALI), mimicking the in vivo alveolar epithelium microenvironment. Integration of a commercially available transwell insert makes the device fabrication straightforward and eliminates the tedious device assembly processes. The transwell insert can later be detached from the device for high-resolution imaging of the cells. In the experiments, the cells showing type-I pneumocyte markers are exploited to construct an in vitro alveolar epithelium model, and four culture conditions including conventional liquid/liquid culture (LLC) and air–liquid interface (ALI) cell culture in normal growth medium, and ALI cell culture with inflammatory cytokine (TNF-α) stimulation and ethanol vapor exposure are applied to investigate their effects on the alveolar epithelium barrier function. The barrier permeability is time-lapse monitored using trans-epithelial electrical resistance (TEER) measurement and immunofluorescence staining of the tight junction protein (ZO-1). The results demonstrate the functionalities of the device, and further show the applications and advantages of the constructed in vitro cell models for the lung studies.

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