Influenza A virus infection modulates the expression of type IV collagenase in epithelial cells

Abstract

We investigated the effect of influenza A/Beijing/353/89 (H3N2) virus infection on the expression of type IV collagenase in two different types of epithelial cell. Depending on the cell line infected, the viral infection caused changes in the expression of type IV collagenase. The expression of matrix metalloproteinase-9 (MMP-9; 92 kDa) but not of matrix metalloproteinase-2 (MMP-2; 72 kDa) was stimulated in Vero cells. In MDCK cells, the MMP-2 production increased with the virus infection. According to the enzymatic activity revealed with zymography, the MMP-9 promoter activity rose by a factor of over 1788 in influenza A virus-infected Vero cells but not in MDCK cells. The tissue inhibitor of metalloproteinase, TIMP-1, had increased slightly (2.3-fold) in Vero cells 48 hours after the infection, but in MDCK cells, influenza A virus had no effect on the TIMP-1 expression. In conclusion, the MMP-9 and -2 expression by influenza A virus infection are modulated at transcriptional level, depending on the epithelial cell line.

Respiratory syncytial virus infection induces matrix metalloproteinase-9 expression in epithelial cells

“…MMP-9 is known to be up-regulated in a variety of cells by many agents, including growth factors, cytokines, and viral proteins [15,22]. In a previous study, we also demonstrated that the influenza A virus modulates MMP-9 expression in epithelial cells [41]. As shown in the results of this study, MMP-9 was divergently regulated depending on the viruses.…”

“…Several reports have described the effect of host proteinase or its inhibitors on the viral infection and multiplication of paramyxoviruses [22,27,38]. We have also reported that the influenza A virus can modulate MMP-9 expression in epithelial cells [41]. Interestingly, the amino acid sequence at the N-terminus of the F1 protein (Gly-Ile/Leu), which is conserved among the paramyxoviruses [38], corresponds to the cleavage site of the substrate recognized by MMP [25].…”

Gene Expression Profiles in Murine Influenza Pneumonia

“…Infection of epithelial cells with influenza induced MMP-2 mRNA expression [42] , while poly(I:C)-stimulated epithelial cells increased secretion of MMP-1, MMPs 8-10 and MMP-13 [36] . MMP-2 and MMP-9 are proteinases which are capable of degrading type IV collagen; thus, they are considered to play an important role in the degeneration of the epithelial cell layer [43] .…”

Mononuclear Phagocyte Differentiation, Activation, and Viral Infection Regulate Matrix Metalloproteinase Expression: Implications for Human Immunodeficiency Virus Type 1-Associated Dementia

“…MMP dysregulation can affect tissue remodeling and organ homeostasis (38,65). In response to inflammation and cellular activation, MMPs are synthesized by a variety of cell types (4,49) including endothelial and epithelial cells (13,64), leukocytes (14,23,34,46), neural cells (38), and hepatocytes (47). Cytokines (11), cell differentiation (6,48,60), and viral infection (11,55) affect the regulation of MMPs.…”