Influenza-pseudotyped Gag virus-like particle vaccines provide broad protection against highly pathogenic avian influenza challenge

Haynes, Joel R.; Dokken, Leslie; Wiley, James; Cawthon, Andrew G.; Bigger, John E.; Harmsen, Allen G.; Richardson, Charles C.

doi:10.1016/j.vaccine.2008.11.011

Cited by 93 publications

(76 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, this identified polybasic aa section adjacent to the cleavage site can be manipulated to create a virus of less pathogenicity for the use in LAIV derivation [10,21,22]. [23][24][25]. Although these vaccines did not show the hoped efficacy [16;21], this vaccination platform represents an important route for further investigation.…”

mentioning

confidence: 99%

The current state of H5N1 vaccines and the use of the ferret model for influenza therapeutic and prophylactic development

Banner

Kelvin²

2012

J Infect Dev Ctries

View full text Add to dashboard Cite

Highly pathogenic avian influenza H5N1 is a threat to global public health as a natural pandemic causing agent but has recently been considered a bioterrorism concern. The evolving view of the H5N1 virus necessitates the re-evaluation of the current status of H5N1 therapeutics and prophylactics, in particular the preparation of viable H5N1 vaccination strategies as well as the use of ferrets in influenza research. Here the highly pathogenic H5N1 virus dilemma is discussed in context with the current H5N1 vaccine status and the use of the ferret model. Previously, the development of various H5N1 vaccine platforms have been attempted, many of them tested in the ferret model, including vector vaccines, adjuvant vaccines, DNA vaccines, and reverse engineered vaccines. Moreover, as ferrets are a superlative animal model for influenza investigation and vaccine testing, it is imperative that this model is recognized for its uses in prophylactic development and not only as an agent for creating transmissible influenza viruses. Elucidating the ferret immune response and creating ferret immune reagents remain important goals in conjunction with the development and manufacture of H5N1 vaccines. In summary, an efficacious H5N1 vaccine is urgently needed and the ferret model remains an appropriate model for its development.J Infect Dev Ctries 2012; 6(6):465-469.

show abstract

mentioning

confidence: 99%

The current state of H5N1 vaccines and the use of the ferret model for influenza therapeutic and prophylactic development

Banner

Kelvin²

2012

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Influenza VLPs are noninfectious particles that mimic the virus in structure and morphology, can be produced using an egg-free cell culture system, and have been shown to be highly immunogenic, inducing protective immunity (9,15,19,27,35,41,42,44). Most current vaccines are administered intramuscularly to humans in liquid formulations using hypodermic needles or syringes.…”

mentioning

confidence: 99%

Intradermal Vaccination with Influenza Virus-Like Particles by Using Microneedles Induces Protection Superior to That with Intramuscular Immunization

Quan

Kim

Vunnava

et al. 2010

J Virol

115

View full text Add to dashboard Cite

Influenza virus-like particles (VLPs) are a promising cell culture-based vaccine, and the skin is considered an attractive immunization site. In this study, we examined the immunogenicity and protective efficacy of influenza VLPs (H1N1 A/PR/8/34) after skin vaccination using vaccine dried on solid microneedle arrays. Coating of microneedles with influenza VLPs using an unstabilized formulation was found to decrease hemagglutinin (HA) activity, whereas inclusion of trehalose disaccharide preserved the HA activity of influenza VLP vaccines after microneedles were coated. Microneedle vaccination of mice in the skin with a single dose of stabilized influenza VLPs induced 100% protection against challenge infection with a high lethal dose. In contrast, unstabilized influenza VLPs, as well as intramuscularly injected vaccines, provided inferior immunity and only partial protection (<40%). The stabilized microneedle vaccination group showed IgG2a levels that were 1 order of magnitude higher than those of other groups and had the lowest lung viral titers after challenge. Also, levels of recall immune responses, including hemagglutination inhibition titers, neutralizing antibodies, and antibody-secreting plasma cells, were significantly higher after skin vaccination with stabilized formulations. Therefore, our results indicate that HA stabilization, combined with vaccination via the skin using a vaccine formulated as a solid microneedle patch, confers protection superior to that with intramuscular injection and enables potential dose-sparing effects which are reflected by pronounced increases in rapid recall immune responses against influenza virus.

show abstract

“…Melo R. Human Epidermal Growth Factor Receptor 2 (HER2) is, among EGFR family, one of the most relevant members as it remains overexpressed on tumor cells and provides resistance to wellstudied anti-HER2 monoclonal antibody, Trastuzumab (Herceptin®), or tyrosine kinase inhibitor [1]. Furthermore, HER2 plays a key role in the HER family due the interaction with other HER receptors via a complex signaling network to regulate cell growth, differentiation and survival [2].…”

Section: Structural Mechanism Of Her2-antibodies Complexes By Moleculmentioning

confidence: 99%

Structural mechanism of HER2-antibodies complexes by molecular dynamics studies

Melo

Almeida

Verde

et al. 2017

Proceedings of MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd Edition

View full text Add to dashboard Cite

Abstract.Human Epidermal Growth Factor Receptor 2 (HER2) is, among EGFR family, one of the most relevant members as it remains overexpressed on tumor cells and provides resistance to wellstudied anti-HER2 monoclonal antibody, Trastuzumab (Herceptin®), or tyrosine kinase inhibitor [1]. Furthermore, HER2 plays a key role in the HER family due the interaction with other HER receptors via a complex signaling network to regulate cell growth, differentiation and survival [2]. In this work, we have employed computational modelling and Molecular Dynamic (MD) simulations to attain a deeper

show abstract

Influenza-pseudotyped Gag virus-like particle vaccines provide broad protection against highly pathogenic avian influenza challenge

Cited by 93 publications

References 52 publications

The current state of H5N1 vaccines and the use of the ferret model for influenza therapeutic and prophylactic development

The current state of H5N1 vaccines and the use of the ferret model for influenza therapeutic and prophylactic development

Intradermal Vaccination with Influenza Virus-Like Particles by Using Microneedles Induces Protection Superior to That with Intramuscular Immunization

Structural mechanism of HER2-antibodies complexes by molecular dynamics studies

Contact Info

Product

Resources

About