Continuing the evolution towards Industry 4.0, the industrial communication protocols represent a significant topic of interest, as real-time data exchange between multiple devices constitute the pillar of Industrial Internet of Things (IIoT) scenarios. Although the legacy protocols are still persistent in the industry, the transition was initiated by the key Industry 4.0 facilitating protocol, the Open Platform Communication Unified Architecture (OPC UA). OPC UA has to reach the envisioned applicability, and it therefore has to consider coexistence with other emerging real-time oriented protocols in the production lines. The Data Distribution Service (DDS) will certainly be present in future architectures in some areas as robots, co-bots, and compact units. The current paper proposes a solution to evaluate the real-time coexistence of OPC UA and DDS protocols, functioning in parallel and in a gateway context. The purpose is to confirm the compatibility and feasibility between the two protocols alongside a general definition of criteria and expectations from an architectural point of view, pointing out advantages and disadvantages in a neutral manner, shaping a comprehensive view of the possibilities. The researched architecture is meant to comply with both performance comparison scenarios and interaction scenarios over a gateway application. Considering the industrial tendencies, the developed solution is applied using non-ideal infrastructures to provide a more feasible and faster applicability in the production lines.