“…Therefore, our protocol combines this final step with the key preliminary assessment of the isoform mapping correctly the mutation of interest. Importantly, all steps of our protocol yield results that can be used at different stages by the user: the identification of specific isoforms containing residues involved in selected mutations is per se a remarkable clue for genetic assessment of the impact of isoforms, especially by handling a large number of proteins and point mutations; the set of the templates eventually identified by MoNvIso with the section of the target protein covered by them are made available to the user; finally, the structural predictions represent a valuable starting point for additional refinements and investigations, such as molecular dynamics simulations ( Raval et al, 2012 ; Hollingsworth and Dror, 2018 ; Lazim et al, 20202020 ; Miller and Phillips, 2021 ; Itoh and Okumura, 2022 ), hot spots evaluation ( Murakami et al, 2017 ; Liu et al, 20182018 ; Rosell and Fernández-Recio, 2018 ; Rosensweig et al, 2018 ), protein-protein docking ( Kangueane and Nilofer, 2018 ; van Noort et al, 2021 ) and more ( Poelwijk et al, 2016 ; Rivoire et al, 2016 ; Salinas and Ranganathan, 2018 ). Finally, note that for isoforms without good quality-templates, users could choose to use predicted structures such as those provided by AF and RosettaFold ( Baek et al, 2021 ) or other modelling packages and/or protocols to build their own structural models using the isoform(s) correctly associated with the selected point mutations.…”