Information Limits of Optical Microscopy: Application to Fluorescent Labelled Tissue Section

Abstract

The article demonstrates some less known principles of image build-up in diffractive microscopy and their usage in analysis unravelling the smallest localized information about the original object -an electromagnetic centroid. In fluorescence, the electromagnetic centroid is naturally at the position of the fluorophore. The usage of an informationentropic variable -a point divergence gain -is demonstrated for finding the most localized position of the object's representation, generally of the size of a voxel (3D pixel). These spatial pixels can be qualitatively classified and used for reconstruction of the 3D structures with precision comparable with electron microscopy.

Point Divergence Gain and Multidimensional Data Sequences Analysis

“…The rest of image escaped the observation. In the next paper [ 25 ], this method was extended to widefield fluorescent data.…”