Infrared light induced patterning of proteins on ppNIPAM thermoresponsive thin films: a “protein laser printer”

Abstract

Controlled adsorption and patterning of proteins on a thin film of thermo-responsive plasma-deposited poly-N-isopropylacrylamide occurs in response to heating with a computer-controlled infrared laser beam, with pattern resolution in the single micrometer range and adsorption times within a few seconds. Deposition of multiple proteins in the same substrate region has been accomplished. Successful streptavidin-biotin and antibody-antigen binding indicates that protein function stays intact after proteins adsorb to the ppNI-PAM bio-polymer surface.

Remote Control of Reversible Localized Protein Adsorption in Microfluidic Devices

“…The detailed experimental process is provided in the Supporting Information. This mixture could be stored and used just like pristine PDMS prepolymer, which is easily cured in any shape or size with appropriate molds, avoiding the coating of additional heat layer. , From Figure S3 in the Supporting Information, we can see the D band (around 1300 cm –1 ) and G band (around 1600 cm –1 ) of graphene appeared among typical PDMS peaks. As shown in Scheme , the embedded NGO functions as photothermal conversion materials due to its high light absorbing efficiency at the visible and near-infrared wavelengths.…”

Programmable Patterning of Protein Bioactivity by Visible Light

“…Thereby, functional protein patterns with different geometries and sizes could be successfully generated in situ. As such, our method complements an approach recently published by Cheng et al, who used a focused infrared laser to create two-dimensional protein micropatterns in a point-scanning manner. However, our results advance this technique by the possibility to use visible light patterned by various shapes of diaphragms.…”

Laser Micro-Printing of Dye-Molecules on Polymeric Surfaces

“…In this technique, the SRG patterns are formed by immobilizing the molecules in the high intensity region of the spot where the two beams interfere. In another work [8], protein laser printing technique is reported using 100 -300 mW infrared laser light with 1 -300 seconds of exposure time to activate polymer thin films for micrometer range biopatterning of aqueous phase protein onto laser exposed spots.…”