Infrared microscopy characterisation of carotid plaques and thyroid tissue biopsies

Alò, F; Bruni, P.; Cavalleri, Amila; Conti, Carla; Giorgini, Elisabetta; Rubini, Corrado; Tosi, Giorgio

doi:10.1016/s0022-2860(02)00660-9

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…By taking into consideration our previous studies on the argument, we have undertaken a micro-FTIR imaging study on plaques with different calcification grading, to further characterize the components of atheromatous plaques and with the aim to localize cytotoxic components to better understand their role in the 'death' zone of the atheromatous plaque [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Micro-FTIR imaging spectroscopy of calcified atheromatous carotid plaques. Part IV

Alò

Conti

Ferraris

et al. 2009

Journal of Molecular Structure

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Section: Introductionmentioning

confidence: 99%

Micro-FTIR imaging spectroscopy of calcified atheromatous carotid plaques. Part IV

Alò

Conti

Ferraris

et al. 2009

Journal of Molecular Structure

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“…New innovative development in IR microscopy (figure 1) has led to favourable technical specifications and a spatial resolution up to 10 × 10 µm 2 , which has resulted in novel and unique applications in the field of bio-medicine [6,7]. Recently, the IR region has been proposed for the study of cells, fluids, micro-organisms and biological tissues [8][9][10][11][12]. It has been found that the IR absorption of normal tissues is different from that of diseased tissues, from which it can be assumed that each diseased state of biological tissue has its own distinct, characteristic IR-spectral pattern.…”

Section: Introductionmentioning

confidence: 99%

Fibre-optic laser-assisted infrared tumour diagnostics (FLAIR)

Bindig¹,

Müller²

2005

J. Phys. D: Appl. Phys.

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Laser based fibre-optic surgery procedures are commonly used in minimal invasive surgery. Despite the development of precise and efficient laser systems there are also innovative attempts in the field of bio-medical diagnostics. As a direct result of the tissue's optical properties most applications are focused on the visible wavelength range of the spectrum. The extension of the spectrum up to the mid-infrared (IR) region will offer a broad range of possibilities for novel strategies with a view to non-invasive diagnostics in medicine. We describe a method to detect differences between diseased and normal tissues, which involve Fourier transform IR microspectroscopy and fibre-optics methods. Regions of interest on 10 µm thin tissue sections were mapped using an IR microscope in transmission mode. After IR-mapping, the samples were analysed using standard pathological techniques. Quadratic discriminant and correlation analyses were applied to the IR maps obtained allowing differentiation between cancerous and normal tissue. The use of optical fibres, transparent in the mid-IR, allowed measurements to be made in the attenuated total reflectance (ATR)-mode at a remote location. The IR sensor is in contact with the sample that shows characteristic absorption lines. The total transmission of the fibre and the sample will decrease at these lines. This method can be used to determine the absorption of a sample in a non-destructive manner. In this paper we report on our efforts to develop an IR fibre-optic sensor for tissue identification as well as to differentiate between malignant and healthy tissue in vivo. We also describe the technical design of the laboratory set-up and the results of developments made. Silver halide fibres and a special sensor tip were used for the ATR measurements on tissue specimens. The results indicate that fibre-optic IR spectrometry will be a useful tool for bio-diagnostics.

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Spectroscopic imaging of arteries and atherosclerotic plaques

et al. 2004

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Fourier transform infrared (FTIR) spectroscopic imaging using a focal plane array detector has been used to study atherosclerotic arteries with a spatial resolution of 3-4 microm, i.e., at a level that is comparable with cellular dimensions. Such high spatial resolution is made possible using a micro-attenuated total reflection (ATR) germanium objective with a high refractive index and therefore high numerical aperture. This micro-ATR approach has enabled small structures within the vessel wall to be imaged for the first time by FTIR. Structures observed include the elastic lamellae of the tunica media and a heterogeneous distribution of small clusters of cholesterol esters within an atherosclerotic lesion, which may correspond to foam cells. A macro-ATR imaging method was also applied, which involves the use of a diamond macro-ATR accessory. This study of atherosclerosis is presented as an illustrative example of the wider potential of these ATR imaging approaches for cardiovascular medicine and biomedical applications.

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Infrared microscopy characterisation of carotid plaques and thyroid tissue biopsies

Cited by 5 publications

References 15 publications

Micro-FTIR imaging spectroscopy of calcified atheromatous carotid plaques. Part IV

Micro-FTIR imaging spectroscopy of calcified atheromatous carotid plaques. Part IV

Fibre-optic laser-assisted infrared tumour diagnostics (FLAIR)

Spectroscopic imaging of arteries and atherosclerotic plaques

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