Infrared <scp>LED</scp> irradiation photobiomodulation of oxidative stress in human dental pulp cells

Montoro, Liege Aldrovandi; Turrioni, Ana Paula Silveira; Basso, Fernanda Gonçalves; Costa, Carlos Alberto de Souza; Hebling, Josimeri

doi:10.1111/iej.12211

Cited by 25 publications

(45 citation statements)

References 49 publications

(112 reference statements)

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“…Based on the analysis of positive data provided by previous studies, researchers have been encouraged to develop more investigations to establish the most beneficial physical parameters of LED or laser irradiation of pulp cells (8)(9)(10)(11)20).…”

Section: Discussionmentioning

confidence: 99%

“…According to Alghamdi et al (12), power values from 1 to 500 mW and energy densities from 0.04 to 50 J/cm 2 are considered low-intensity therapy parameters, recommended for the irradiation of cells in culture. Therefore, the irradiation parameters used in this study followed the recommendations of Alghamdi et al (12) as well as those of Montoro et al (9), who studied modulation of reactive oxygen species (ROS) and nitric oxide (NO) production by human dental pulp cells subjected to infrared LED therapy.…”

Section: Discussionmentioning

confidence: 99%

“…For cell irradiation, a previously described standardized device (LEDtable) containing 24 infrared diodes (850 nm) was used (9)(10)(11). The irradiance was fixed at 40 mW/cm 2 , and the irradiation times were 50 s (2 J/cm 2 ), 1 min and 40 s (4 J/cm 2 ), 3 min and 20 s (8 J/cm 2 ), 6 min and 15 s (15 J/cm 2 ) and 12 min and 30 s (30 J/cm 2 ) (9).…”

Section: Irradiation Devicementioning

confidence: 99%

“…Low-level light therapy (LLLT), with lasers or light-emitting diodes (LED), has been widely used in dentistry for tissue and cell stimulation (5)(6)(7)(8)(9)(10)(11). Concerning the pulp tissue or pulp cells subjected to phototherapy, several authors have reported encouraging scientific data, such as increased deposition of tertiary dentin (5), enhanced cell viability and proliferation (8,11), and biomodulation of inflammatory cytokines and reactive oxygen species (9). Despite these positive results, a major challenge in tissue biostimulation involving phototherapy is to define the optimal window for each cell type and desired action (3).…”

Section: Introductionmentioning

confidence: 99%

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Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

et al. 2015

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Despite several reports regarding tissue regeneration, including pulp repair induced by different light sources, only limited data have been reported concerning the effects of light-emitting diodes (LED) on stem cells from human exfoliated deciduous teeth (SHEDs). The aim of this study was to evaluate the effects of different energy densities of infrared LED on the cell viability, number of cells and mineralized tissue production by SHEDs. SHEDs were obtained from near-exfoliation primary teeth (n=3), seeded in plain DMEM (10 4 cells/cm 2 ), and irradiated by a LED prototype (LEDTable 850 nm, 40 mW/cm 2 ) delivering 0 (control), 2, 4, 8, 15 or 30 J/cm 2 (n=9). Cell viability (MTT assay), cell proliferation (trypan blue assay), and mineralized nodule (MN) formation (alizarin red stain) were assessed 12 and 72 h post-irradiation. Data were subjected to Kruskal-Wallis and Mann-Whitney tests (α=0.05). Cells irradiated with 2 or 4 J/cm 2 exhibited higher metabolism at 72 h, and all energy densities provided increase in cell proliferation after 12 h. Regarding MN formation, the best results were observed at 72 h after SHED irradiation with 8 and 15 J/cm 2 . It was concluded that the cell viability, cell number and MN formation by pulp cells are enhanced after exposure to infrared LED irradiation. Overall, the greatest SHED biostimulation was obtained with 4 and 8 J/cm 2 . D o s e -r e s p o n s e s o f S t e m C e l l s f r o m H u m a n E x f o l i a t e d Te e t h t o I n f r a r e d L E D I r r a d i a t i o n

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Irradiation Devicementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

et al. 2015

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“…The energy densities (EDs) selected for this study were 0.5, 2, 4, 10, and 15 J/cm 2 , according to previous researches conducted in different types of cells [6,11,12,[18][19][20][21][22][23][24]. Since the time of irradiation varied according to the ED, due to the fact that the irradiance was fixed, a corresponding control group was delineated for each ED (Table 1), in which the cells were kept out of the incubator for the same period of time than the corresponding irradiated group.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED

et al. 2016

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To evaluate the effect of irradiation with light-emitting diode (LED; 455 nm) on the viability and synthesis of dentin matrix proteins by odontoblast-like cells, MDPC-23 cells were cultivated (10(4) cells/cm(2)) in 24-well culture plates. After 12 h incubation in Dulbecco's modified Eagle's medium (DMEM), the cells were submitted to nutritional restriction by means of reducing the concentration of fetal bovine serum (FBS) for an additional 12 h. Cells were irradiated one single time with one of the following energy densities (EDs): 0.5, 2, 4, 10, or 15 J/cm(2) and irradiance fixed at 20 mW/cm(2). Non-irradiated cells served as control. After 72 h, cells were evaluated with regard to viability (methylthiazol tetrazolium technique (MTT)), mineralization nodule (MN) formation, total protein (TP) production, alkaline phosphatase activity (ALP), and collagen synthesis (Sircol), n = 8. The data were submitted to Kruskal-Wallis and Mann-Whitney tests (p > 0.05). There was no statistical difference between the viability of cells irradiated or not (control), for all the EDs. However, an increase in TP was observed for all the EDs when compared with the control group. A reduced ALP activity was seen in all irradiated groups, except for the ED of 0.5 J/cm(2), which did not differ from the control. There was no difference between the irradiated groups and control regarding collagen synthesis, with the exception of the ED of 10 J/cm(2), which inhibited this cell function. Significant reduction in MN occurred only for the EDs of 0.5 and 2 J/cm(2). The single irradiation with blue LED (455 nm), irradiance of 20 mW/cm(2), and energy densities ranging from 0.5 to 15 J/cm(2) exerted no effective biostimulatory capacity on odontoblast-like cells.

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Development and application of LED arrays for use in phototherapy research

Cooper

Milward

Gorecki

et al. 2017

Journal of Biophotonics

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Lasers/LEDs demonstrate therapeutic effects for a range of biomedical applications. However, a consensus on effective light irradiation parameters and efficient and reliable measurement techniques remain limited. The objective here is to develop, characterise and demonstrate the application of LED arrays in order to progress and improve the effectiveness and accuracy of in vitro photobiomodulation studies. 96-well plate format LED arrays (400-850 nm) were developed and characterised to accurately assess irradiance delivery to cell cultures. Human dental pulp cells (DPCs) were irradiated (3.5-142 mW/cm : 15-120 s) and the biological responses were assessed using MTT assays. Array calibration was confirmed using a range of optical and analytical techniques. Multivariate analysis of variance revealed biological responses were dependent on wavelength, exposure time and the post-exposure assay time (P < 0.05). Increased MTT asbsorbance was measured 24 h post-irradiation for 30 s exposures of 3.5 mW/cm at 470, 527, 631, 655, 680, 777, 798 and 826 nm with distinct peaks at 631 nm and 798 nm (P < 0.05). Similar wavelengths were also effective at higher irradiances (48-142 mW/cm ). LED arrays and high throughput assays provide a robust and reliable platform to rapidly identify irradiation parameters which is both time- and cost-effective. These arrrays are applicable in photobiomodulation, photodynamic therapy and other photobiomedical research.

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Infrared LED irradiation photobiomodulation of oxidative stress in human dental pulp cells

Abstract: Biomodulation of oxidative stress of HPDC can be achieved by irradiation with a single dose of infrared LED. Within the range investigated, 15 J cm(-2) resulted in the least production of NO.

Cited by 25 publications

References 49 publications

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

Dose-responses of Stem Cells from Human Exfoliated Teeth to Infrared LED Irradiation

Synthesis of dental matrix proteins and viability of odontoblast-like cells irradiated with blue LED

Development and application of LED arrays for use in phototherapy research

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