Infrared spectrochemical findings on intermittent fasting-associated gross molecular modifications in rat myocardium

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This study aimed to explore the impact of SCD Probiotics supplementation on biomolecule profiles and histopathology of ileum and colon tissues during a 30‐day intermittent fasting (IF) program. Male Sprague–Dawley rats, aged 24 months, underwent 18‐h daily fasting and received 3 mL (1 × 108 CFU) of SCD Probiotics. The differences in biomolecule profiles were determined using FTIR Spectroscopy and two machine learning techniques, Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM), which showed significant differences with high accuracy rates. Spectrochemical bands indicating alterations in lipid, protein and nucleic acid profiles in both tissues. The most notable changes were observed in the group subjected to both IF and SCD Probiotics, particularly in the colon. Both interventions, individually and in combination, decreased protein carbonylation levels. SCD Probiotics exerted a more substantial impact on membrane dynamics than IF alone. Additionally, both IF and SCD Probiotics were found to have protective effects on intestinal structure and stability by reducing mast cell density and levels of TNF‐α and NF‐κB expression in ileum and colon tissues, thus potentially mitigating age‐related intestinal damage and inflammation. Furthermore, our results illustrated that while IF and SCD Probiotics individually instigate unique changes in ileum and colon tissues, their combined application yielded more substantial benefits. This study provides evidence for the synergistic potential of IF and SCD Probiotics in combating age‐related intestinal alterations.

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 85%

Supplementing probiotics during intermittent fasting proves more effective in restoring ileum and colon tissues in aged rats

Teker,

Ceylani,

Keskin

et al. 2024

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“…The changes in spectrochemical indices associated with the acyl chain of fatty acids (A 2922 /A 2955 ), phosphorylation (A 1239 /A 2955 and A 1080 /A 1545 ), and carbonylation (A 1740 /A 1545 ) of proteins 16 are shown in Figure 5 . Interestingly, IF and SCD Probiotics had the opposite effect on the acyl chain of fatty acids.…”

Section: Resultsmentioning

confidence: 99%

Reduced liver damage and fibrosis with combined SCD Probiotics and intermittent fasting in aged rat

Teker,

Ceylani,

Keskin

et al. 2023

Self Cite

This study aimed to examine the impact of SCD Probiotics supplementation on liver biomolecule content and histological changes during a 30‐day intermittent fasting (IF) program in 24‐month‐old male Sprague–Dawley rats. Rats underwent 18‐h daily fasting and received 1 × 108 CFU of SCD Probiotics daily. Liver tissue biomolecules were analysed using FTIR Spectroscopy, LDA, and SVM techniques, while histopathological evaluations used Haematoxylin and eosin and Masson trichrome‐stained tissues. Blood samples were collected for biochemical analysis. Gross alterations in the quantity of biomolecules were observed with individual or combined treatments. LDA and SVM analyses demonstrated a high accuracy in differentiating control and treated groups. The combination treatments led to the most significant reduction in cholesterol ester (1740 cm−1) and improved protein phosphorylation (A1239/A2955 and A1080/A1545) and carbonylation (A1740/A1545). Individually, IF and SCD Probiotics were more effective in enhancing membrane dynamics (Bw2922/Bw2955). In treated groups, histological evaluations showed decreased hepatocyte degeneration, lymphocyticinfiltration, steatosis and fibrosis. Serum ALP, LDH and albumin levels significantly increased in the SCD Probiotics and combined treatment groups. This study offers valuable insights into the potential mechanisms behind the beneficial effects of IF and SCD Probiotics on liver biomolecule content, contributing to the development of personalized nutrition and health strategies.

“…The seven cross‐validation segments were used to calculate training and cross‐validation accuracies. Finally, the generated training dataset was applied to all sample datasets to obtain an SVM classification model 19 …”

Section: Materials Methodsmentioning

confidence: 99%

The rejuvenating influence of young plasma on aged intestine

Ceylani,

Teker,

Keskin

et al. 2023

Self Cite

This study aims to investigate the effects of plasma exchange on the biomolecular profiles and histology of ileum and colon tissues in young and aged Sprague–Dawley male rats. Fourier transform infrared (FTIR) spectroscopy, linear discriminant analysis and support vector machine (SVM) techniques were employed to analyse the lipid, protein, and nucleic acid indices in young and aged rats. Following the application of young plasma, aged rats demonstrated biomolecular profiles similar to those of their younger counterparts. Histopathological and immunohistochemical assessments showed that young plasma had a protective effect on the intestinal tissues of aged rats, increasing cell density and reducing inflammation. Additionally, the expression levels of key inflammatory mediators tumour necrosis factor‐alpha and cyclooxygenase‐2 significantly decreased after young plasma administration. These findings underscore the therapeutic potential of young plasma for mitigating age‐related changes and inflammation in the intestinal tract. They highlight the critical role of plasma composition in the ageing process and suggest the need for further research to explore how different regions of the intestines respond to plasma exchange. Such understanding could facilitate the development of innovative therapies targeting the gastrointestinal system, enhancing overall health during ageing.