Infusão intramamária de Weissella confusa afeta a contagem de células somáticas e a contagem diferencial de leucócitos no leite

A teat bio-sealant was developed using Weissella cibaria, and the bio-sealant's technological and functional properties were assessed. The development included four experimental phases that were analyzed using independent experimental designs. Initially, sterilized or pasteurized Aloe vera gels were used, and the effect of heat treatment was investigated. In the second phase, the effects of time, storage temperature, and addition of cryopreservatives on the viability of the probiotic were observed. The third phase consisted of evaluating the synergistic effects of the cryopreservatives. The fourth phase involved selecting a material that would provide viscosity to the teat sealant. Technological and functional properties were measured in terms of viability of W. cibaria, and antimicrobial activity against Staphylococcus aureus and Streptococcus agalactiae was also analyzed. A mixture of milk powder and glycerol preserved this antimicrobial activity. Pullulan provided greater viscosity and maintained the technological and functional properties of the bio-sealant for 29 days. This teat bio-sealant can be used as an alternative for the prevention of bovine mastitis.

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Development of a Teat Bio-sealant and Evaluation of its Technological and Functional Properties

Serna-Cock

Pabón-Rodríguez

2016

Probiotics & Antimicro. Prot.

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Nachweis von Weissella spp. in Viertelgemelksproben von zwei Milchkühen mit klinischer Mastitis

Wald¹,

Baumgartner²,

Urbantke³

et al. 2016

Tierarztl Prax Ausg G

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This case report describes the isolation and differentiation of Weissella (W.) spp. from the milk of two cows (A and B) with clinical mastitis (milk changes, asymmetry of the udder and increased somatic cell counts). Quarter milk samples obtained from two dairy cows of different farms had been submitted to the diagnostic laboratory of the Clinic for Ruminants in Vienna for bacteriological examination. Alpha-hemolytic catalase-negative gram-positive cocci in pure culture on Columbia blood agar were isolated and could not be assigned to a Lancefield group. The isolates were biochemically characterized as Leuconostoc spp. (API 20 Strep, bioMérieux). A control examination of cow B within 7 weeks confirmed these findings. 16S rDNA sequencing indicated W. paramesenteroides (cow A) and W. cibaria (cow B). The analysis by pulsed-field gel electrophoresis (PFGE) showed identical SmaI/ApaI profiles for both W. cibaria isolates (cow B), which differed from the W. paramesenteroides fingerprint of cow A (67% similarity). This study indicates a possible relationship between the detection of Weissella spp. and the occurrence of bovine intramammary infections.

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Infusão intramamária de Weissella confusa afeta a contagem de células somáticas e a contagem diferencial de leucócitos no leite

Abstract: The use of lactic acid bacteria (LAB) as an alternative to antimicrobial usage has been proposed for the control of bovine mastitis. However, before its application, the in vivo effects

Cited by 2 publications

References 14 publications

Development of a Teat Bio-sealant and Evaluation of its Technological and Functional Properties

Development of a Teat Bio-sealant and Evaluation of its Technological and Functional Properties

Nachweis von Weissella spp. in Viertelgemelksproben von zwei Milchkühen mit klinischer Mastitis

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