Ingestion of miso regulates immunological robustness in mice

Abstract

In Japan, there is a long history of consumption of miso, a fermented soybean paste, which possesses beneficial effects on human health. However, the mechanism behind these effects is not fully understood. To clarify the effects of miso on immune cells, we evaluated its immunomodulatory activity in mice. Miso did not alter the percentage of B and T cells in the spleen; however, it increased CD69+ B cells, germinal center B cells and regulatory T cells. Anti-DNA immunoglobulin M antibodies, which prevent autoimmune disease, were increased following ingestion of miso. Transcriptome analysis of mouse spleen cells cultured with miso and its raw material revealed that the expression of genes, including interleukin (IL)-10, IL-22 and CD86, was upregulated. Furthermore, intravital imaging of the small intestinal epithelium using a calcium biosensor mouse line indicated that miso induced Ca2+ signaling in a manner similar to that of probiotics. Thus, ingestion of miso strengthened the immune response and tolerance in mice. These results appear to account, at least in part, to the salubrious effects of miso.

Long-term administration of Tetragenococcus halophilus No. 1 over generations affects the immune system of mice

“…The administration of miso in mice for 4 weeks increased the proportion of GC B cells and Treg cells [ 10 ]. In this study, feeding mice with diets containing T .…”

“…1 may contribute to these immunomodulatory effects of miso together with the other ingredients of miso. In contrast, although the administration of miso for 12 weeks did not increase the proportion of activated T cells or Tfh cells [ 10 ], administration of T . halophilus No.…”

“…1 in the F2 generation of the mice decreased the proportion of anti-DNA antibodies of IgG class, which are pathogenic. Although it is mild, miso possesses a protective effect against autoimmunity [ 10 ]. Therefore, T .…”

“…The consumption of miso soup could be linked to the overall health and life span of Japanese people. Furthermore, we showed that immunological robustness is enhanced by the consumption of miso [ 10 ]. We also identified Tetragenococcus halophilus No.…”

“…1 derived from miso as a potent immunomodulator [ 11 ]. Furthermore, in a study on the visualization of Ca 2+ signaling in intestinal epithelial cells in vivo , the response of lactic acid bacteria was found to be similar to miso [ 10 , 12 ]. Based on these findings, we believe that some components in miso affect its constitution over generations and that the main ingredient may be lactic acid bacteria.…”