Ingestion of potato starch decreases chymotrypsin but does not affect trypsin, amylase, or lipase activity in the pancreas in rats

Mineo, Hitoshi; Ishida, Kyo; Morikawa, Nao; Ohmi, Sayako; Machida, Ayaka; Noda, Takahiro; Fukushima, Michihiro; Chiji, Hideyuki

doi:10.1016/j.nutres.2006.12.011

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…Successful application of protease inhibitors to stimulate CCK release in humans most likely requires a combination of trypsin, chymotrypsin and elastase inhibition, 18 and inhibition of trypsin activity alone is probably not sufficient. [18][19][20] Furthermore, the inhibitors have to be resistant to the enzymatic activity of human gastric proteases. Our in vitro data show that PI2 indeed inhibited the action of trypsin, chymotrypsin and elastase under simulated gastric conditions, and data supplied by the manufacturers also confirmed that both the amount and inhibitory activity of PI2 in the product were not affected by processing of the minidrink.…”

Section: Discussionmentioning

confidence: 99%

The effect of protease inhibitors derived from potato formulated in a minidrink on appetite, food intake and plasma cholecystokinin levels in humans

et al. 2010

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Section: Discussionmentioning

confidence: 99%

The effect of protease inhibitors derived from potato formulated in a minidrink on appetite, food intake and plasma cholecystokinin levels in humans

et al. 2010

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“…The trypsin (TPS) activities in serum and tissue samples were assayed at 410 nm using the substrate N-benzoyl-DL-arginine p-nitroaniline hydrochloride (BAPNA) (A080-2-2, Nanjing Jiancheng Bioengineering Institute, Nanjing, Jiangsu, China) according to the manufacturer’s instructions [ 21 ]. TPS could react with BAPNA to release p-nitroaniline with a maximum absorption peak at 410 nm.…”

Section: Methodsmentioning

confidence: 99%

Effects of Soybean Trypsin Inhibitor on Pancreatic Oxidative Damage of Mice at Different Growth Periods

Yang

Li³

et al. 2023

Foods

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The bioactive components in soybeans have significant physiological functions. However, the intake of soybean trypsin inhibitor (STI) may cause metabolic disorders. To investigate the effect of STI intake on pancreatic injury and its mechanism of action, a five-week animal experiment was conducted, meanwhile, a weekly monitor on the degree of oxidation and antioxidant indexes in the serum and pancreas of the animals was carried out. The results showed that the intake of STI had irreversible damage to the pancreas, according to the analysis of the histological section. Malondialdehyde (MDA) in the pancreatic mitochondria of Group STI increased significantly and reached a maximum (15.7 nmol/mg prot) in the third week. Meanwhile, the antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), trypsin (TPS), and somatostatin (SST) were decreased and reached minimum values (10 U/mg prot, 87 U/mg prot, 2.1 U/mg prot, 10 pg/mg prot) compared with the Group Control. The RT-PCR results of the expression of SOD, GSH-Px, TPS, and SST genes were consistent with the above. This study demonstrates that STI causes oxidative structural damage and pancreatic dysfunction by inducing oxidative stress in the pancreas, which could increase with time.

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“…α Amylase activity in the tissue homogenate was determined by the production of maltose from soluble starch (Wako). 22) Briefly, the homogenate was diluted with cold 154 mM NaCl solution (1 vol:100 vol), and then the 50 µL homogenate dilution was mixed with 500 µL of 0.1% soluble starch solution and 450 µL of phosphate buffer at pH 7.4 in a test tube. The mixture was incubated for 10 min at 37 C in a water bath, and then the maltose concentration in the mixture was determined by the modified Nelson Somogyi method.…”

Section: Methodsmentioning

confidence: 99%

Feeding of Potato Starch Increases Maltase and Sucrase Activity Only in Duodenal Segment of the Small Intestine in Rats

Mineo¹,

Kanazawa²,

Morikawa³

et al. 2008

J. Appl. Glycosci.

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Carbohydrates in food are digested by hydrolytic enzymes in the lumen of the small intestine. α Amylase in the pancreatic juice and disaccharidases on the brush border membranes in the small intestine are the main enzymes for the digestion of dietary carbohydrates. The activity of hydrolytic enzymes on carbohydrates and their regulation by dietary components has already been reviewed in detail for the pancreas 1,2) and the small intestine. 3,4) Pancreatic α amylase first cleaves internal α 1,4 bonds in polysaccharides and produces maltose, maltotriose and maltotetraose in upper portion of the lumen in the small intestine. The second stage of carbohydrate digestion occurs on the surface of intestinal epithelial cells in microvillous membrane. Several glycosidases, or disaccharidases, hydrolyze di or oligosaccharides derived from ingested carbohydrates to smaller saccharides. The final products of carbohydrate digestion are monosaccharides, which are then absorbed into the epithelial cell lining inner surface of the intestine. Aging, fasting and feeding conditions influence the activities of pancreatic α amylase 1,2) and intestinal disaccharidases. 3,4) Characteristics of the ingested carbohydrates also have a significant effect on pancreatic α amylase 5,6) and intestinal disaccharidase activities. 7,8) Certain types of diet were reported to affect both pancreatic α amylase and intestinal disaccharidases at the same time. 9,10) Carbohydrates are the main energy source and are usu

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Ingestion of potato starch decreases chymotrypsin but does not affect trypsin, amylase, or lipase activity in the pancreas in rats

Cited by 4 publications

References 33 publications

The effect of protease inhibitors derived from potato formulated in a minidrink on appetite, food intake and plasma cholecystokinin levels in humans

The effect of protease inhibitors derived from potato formulated in a minidrink on appetite, food intake and plasma cholecystokinin levels in humans

Effects of Soybean Trypsin Inhibitor on Pancreatic Oxidative Damage of Mice at Different Growth Periods

Feeding of Potato Starch Increases Maltase and Sucrase Activity Only in Duodenal Segment of the Small Intestine in Rats

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