Inhalation exposure to 1,2-dichloropropane: Distribution of blood and tissue concentrations of 1,2-dichloropropane in rats during and after exposure

Abstract

The present investigation was undertaken to determine the distribution and accumulation of 1,2-dichloropropane (DCP) in the blood, lung, liver, kidney, and abdominal fat of rats during and after inhalation exposure. Male rats were exposed to 80 or 500 ppm (v/v) DCP vapor for 360 min and the concentrations of DCP in the blood and tissues during the inhalation exposure period and after the end of the exposure period were measured. DCP accumulation in the abdominal fat was much greater than that in the blood and other tissues. Eighteen hours after the end of inhalation exposure, DCP could still be detected in the abdominal fat in the 80-ppm group, and in the blood, liver, kidney, and abdominal fat in the 500-ppm group. Our results are valuable data pertaining to the pharmacokinetics of DCP and to human health risk assessment of exposure to DCP vapor by inhalation.

Distribution of 1,2-dichloropropane in blood and other tissues of rats after oral administration

“…Timchalk et al (1991) reported that in rats [ 14 C]DCP could still be detected in the body 24 and 48 hr after oral administration of 1 or 100 mg/kg body. In addition, we previously reported that in rats DCP was still detected in the blood 1080 min after the end of a 6-hr inhalation exposure period to 80 or 500 ppm DCP vapor (Take et al, 2014b). Taken together, these data (the present study; Take et al, 2014b;Timchalk et al, 1991) suggest that in rats DCP remains in the blood for a prolonged period of time after administration.…”

“…Timchalk et al (1991) reported that [ 14 C]DCP was still detectable in the tissues of rats administered 1 or 100 mg/kg body weight 24-and 48-hr after oral administration, and that [ 14 C]DCP was still detectable in the tissues of rats exposed to 5, 50, or 100 ppm [ 14 C]DCP vapor for a 6-hr period 42 hrs after the end of the exposure period. We previously reported that DCP was still detectable in the tissues of rats exposed to 80 or 500 ppm DCP vapor for a 6-hr period 1080 min after the end of the exposure period (Take et al, 2014b). In our present study, DCP could still be detected in the tissues 1440 min after oral administration.…”

“…Six rats were used for each collection. 0.2 mL blood samples were collected into 10-mL headspace sampler (HS)-vials, and 0.2 mL of distilled water was added to each sample (Take et al, 2009(Take et al, , 2010(Take et al, , 2012(Take et al, , 2014a(Take et al, , 2014b. The lung, liver, kidney, and abdominal fat were removed from each rat, and tissue samples (range from about 0.2 to 0.6 g) were placed into separate 10-mL HS-vials.…”

“…The lung, liver, kidney, and abdominal fat were removed from each rat, and tissue samples (range from about 0.2 to 0.6 g) were placed into separate 10-mL HS-vials. Then, 5 mL of distilled water was added to the vials, and the vials were immediately sealed with an aluminum crimp cup (Take et al, 2010(Take et al, , 2012(Take et al, , 2014b.…”

“…Until 2013, only limited information was available about the distribution of DCP in the blood and tissues after inhalation and oral administration (Timchalk et al, 1991). In 2014, we reported the timecourse changes of DCP concentrations in the blood and tissues of rats exposed to 80 or 500 ppm DCP vapor (Take et al, 2014b), concentrations corresponding to the low and high doses used in the two-year inhalation study by Umeda et al (2010).…”