“…[25][26][27] After a brief assessment of the mice in their home cages (activity, rearing, eating, climbing, stereotypy, grooming, and abnormal movements), each animal was removed and held on the observer's hand for scoring ease of removal, handling reactivity, and general appearance. Each mouse was then placed in an exploratory white box (60 Â90 cm), where the observer analyzed central nervous system activity (arousal, rearing), autonomic effects (urination, defecation), muscle tone/equilibrium (gait, mobility, righting reflex, inverted screen), and sensorimotor reactivity (approach response, click response, touch response).…”