Inhaled gases affect the ultrasound contrast produced by albunex® in anesthetized dogs

Wible, James H.; Wojdyla, Jolette K.; Bales, Gary L.; McMullen, William N.; Geiser, Edward A.; Buss, Daryl D.

doi:10.1016/s0894-7317(96)90115-7

Cited by 28 publications

(14 citation statements)

References 9 publications

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“…In such methods the quantification of bound tMBs strongly depends on the injected dose, imaging system gain, and local perfusion [137]. In addition, the influence of inhaled gases in the anaesthetic protocols influences the MBs longevity [148][149][150][151]. These studies confirmed longer circulation times of in-house lipid-shell decafluorobutane-filled UCAs and commercially available UCAs such as Definity and Albunex when animals breathe medical air instead of pure oxygen as the carrier gas for the isoflurane anaesthetic.…”

Section: Contrast-specific Imaging Techniquesmentioning

confidence: 74%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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Ultrasound contrast agents (UCAs) are used routinely in the clinic to enhance contrast in ultrasonography. More recently, UCAs have been functionalised by conjugating ligands to their surface to target specific biomarkers of a disease or a disease process. These targeted UCAs (tUCAs) are used for a wide range of pre-clinical applications including diagnosis, monitoring of drug treatment, and therapy. In this review, recent achievements with tUCAs in the field of molecular imaging, evaluation of therapy, drug delivery, and therapeutic applications are discussed. We present the different coating materials and aspects that have to be considered when manufacturing tUCAs. Next to tUCA design and the choice of ligands for specific biomarkers, additional techniques are discussed that are applied to improve binding of the tUCAs to their target and to quantify the strength of this bond. As imaging techniques rely on the specific behaviour of tUCAs in an ultrasound field, it is crucial to understand the characteristics of both free and adhered tUCAs. To image and quantify the adhered tUCAs, the state-of-the-art techniques used for ultrasound molecular imaging and quantification are presented. This review concludes with the potential of tUCAs for drug delivery and therapeutic applications.

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Section: Contrast-specific Imaging Techniquesmentioning

confidence: 74%

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Rooij

Daeichin

Skachkov

et al. 2015

International Journal of Hyperthermia

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“…MB formulation affects both kinetics and the MBs’ sensitivity to ultrasound, causing different elimination rates [6–8]; however, MB elimination is also affected by the inhaled gases, particularly oxygen (O 2 ), used when performing in vivo studies [9–11]. Former studies focused on Albunex and Optison, which have albumin shells, and Albunex is air filled.…”

Section: Introductionmentioning

confidence: 99%

“…Fortunately, the majority of human studies and the animal studies done with injectable anesthesia are performed with the subject breathing room air. In few patients’ studies and in the majority of rodent studies, imaging is performed with 100% O 2 inhalation that negatively impacts MB survival [11]. Gas anesthesia is preferred, particularly in small animals, because injectable anesthesia impacts homeostasis and provides a narrow safety margin [12].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Inhaled Gases on Ultrasound Contrast Agent Longevity In Vivo

Itani

Mattrey

2011

Mol Imaging Biol

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PurposeThe purpose of this study is to investigate the effect of the inhaled gas used alongside isoflurane in the anesthetization of small animals on the time-intensity curves (TICs) acquired from ultrasound contrast agents—microbubbles.ProceduresTICs were recorded over the common iliac vein of 12 mice receiving Definity®. Animals were anesthetized with isoflurane, the ventilator was driven by medical air (MA), then in random order, the driving gas was changed for 3 min to: MA (control); pure oxygen (O2); O2 + perfluorohexane (PFH:O2); or O2 + octafluoropropane (OFP:O2), the perfluorocarbon (PFC) in Definity, followed by a return to MA 3 min later.ResultsThe mean slope of signal decay was −0.47, −1.05, −1.16, and −1.42 video-intensity units/s for MA, OFP:O2, PFH:O2, and O2, respectively; MA had the slowest decay (p < 0.0001). Both PFC mixtures had slower signal decay than O2, but only OFP:O2 was significant (p < 0.01). When MA was used immediately following dosing, slope gradually decreased (p = 0.032) and was two times slower by the fourth injection (p = 0.012).ConclusionsMicrobubble kinetics are closely associated with the driving gas for inhaled anesthesia. MA has the least effect and should be used when inhaled anesthesia is used. Furthermore, when animals are given multiple injections in the same session, microbubbles last longer with subsequent injections.

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“…This lack of contrast in the left ventricle is probably due to both the "sieve" effect in which the lung capillaries filter out large microbubbles (Meltzer et al 1980), and increased dissolution rates as the microbubbles pass through the lungs (Wible et al 1996;Kabalnov et al 1998). As a result, few microbubbles survive the transit through the lung vasculature to provide contrast in the left ventricle.…”

Section: In Vivo Imagingmentioning

confidence: 99%

In vivo imaging of microfluidic-produced microbubbles

Dhanaliwala

Dixon

Lin

et al. 2015

Biomed Microdevices

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Microfluidics-based production of stable microbubbles for ultrasound contrast enhancement or drug/gene delivery allows for precise control over microbubble diameter but at the cost of a low production rate. In situ microfluidic production of microbubbles directly in the vasculature may eliminate the necessity for high microbubble production rates, long stability, or small diameters. Towards this goal, we investigated whether microfluidic-produced microbubbles directly administered into a mouse tail vein could provide sufficient ultrasound contrast. Microbubbles composed of nitrogen gas and stabilized with 3 % bovine serum albumin and 10 % dextrose were injected for 10 seconds into wild type C57BL/6 mice, via a tail-vein catheter. Short-axis images of the right and left ventricle were acquired at 12.5 MHz and image intensity over time was analyzed. Microbubbles were produced on the order of 10(5) microbubbles/s and were observed in both the right and left ventricles. The median rise time, duration, and decay time within the right ventricle were 2.9, 21.3, and 14.3 s, respectively. All mice survived the procedure with no observable respiratory or heart rate distress despite microbubble diameters as large as 19 μm.

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Inhaled gases affect the ultrasound contrast produced by albunex® in anesthetized dogs

Cited by 28 publications

References 9 publications

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

The Effect of Inhaled Gases on Ultrasound Contrast Agent Longevity In Vivo

In vivo imaging of microfluidic-produced microbubbles

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