Inhaled hydrogen gas therapy for prevention of testicular ischemia/reperfusion injury in rats

Lee, Jae Won; Kim, Jong-In; Lee, Young Ah; Lee, Dong Hun; Song, Chang‐Seon; Cho, Yoon Ju; Han, Jin Soo

doi:10.1016/j.jpedsurg.2011.09.035

Cited by 37 publications

(20 citation statements)

References 40 publications

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“…Hydrogen (H 2 ) has been considered as a potent free radical scavenger and has been demonstrated to have effective protection against tissue damage such as myocardial injury induced by irradiation . Its protective effect is largely due to its ability to limit lipid oxidation and apoptosis . However, no study has investigated the role of H 2 in testicular injury induced by irradiation.…”

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confidence: 99%

Protection by Hydrogen Against Gamma Ray‐Induced Testicular Damage in Rats

Jiang

Yang

et al. 2012

Basic Clin Pharma Tox

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The aim of this study was to investigate the possible protective role of hydrogen-rich saline solution (HRSS) and WR-2721 on the testicular damage induced by irradiation. Sprague-Dawley rats were randomly divided into four groups. Group I served as control group. Rats in group II were exposed to the irradiation. The animals in group III and IV were injected intraperitoneally with HRSS (5 ml/kg) and WR-2721 (200 mg/kg), respectively, 15 min. before the start of gamma irradiation. Testis weight, testis dimensions, sperm count, sperm motility, apoptosis index and biochemical assays were assessed after a 4-day initiation of irradiation. Testis weight, testis dimensions, sperm count, sperm motility in group II were significantly lower compared with those in the control group, whereas they were higher in the HRSS and WR-2721 group. Apoptosis index was significantly increased in group II. Treatment of rats with HRSS and WR-2721 significantly reduced the apoptosis index. On the other hand, irradiation markedly decreased activities of SOD. Activities of SOD were significantly improved when treated with HRSS and WR-2721. Significant increase in the MDA level was observed in group II. MDA levels of group III and IV were significantly lowered when compared with group II. HRSS also played a significant role in the recovery of serum testosterone levels. The results from this experimental study suggest that hydrogen has a possible protective effect against radiation-induced testicular damage.

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confidence: 99%

Protection by Hydrogen Against Gamma Ray‐Induced Testicular Damage in Rats

Jiang

Yang

et al. 2012

Basic Clin Pharma Tox

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“…Lee et al[107] studied the possible therapeutic properties of inhaled 2% hydrogen in pubertal rat model underwent testicular I/R injury. The results of histopathological and biochemical studies suggested that inhalation of hydrogen gas has anti-apoptotic and anti-oxidant properties in cases of TT.…”

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confidence: 99%

Antioxidants in experimental ischemia-reperfusion injury of the testis: Where are we heading towards?

Vaos

Zavras

2017

WJM

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Testicular torsion (TT) is a medical emergency that primary affects newborns and young adolescents. It causes testicular injury due to the torsion of the spermatic cord and its components, initially in the venous blood flow and finally in the arterial blood flow. Prompt diagnosis and early surgical management are necessary in managing this urgent situation. The process of the pathophysiological events in ischemia-reperfusion is multifactorial and deals with the perception of the oxidative stress responsible for the consequences of ischemia/reperfusion (I/R) stress following TT. Duration and severity of torsion also play a significant role in the oxidative stress. A detrimental result of the defense system of the testes takes place resulting finally in testicular atrophy and impaired function. Antioxidant factors have been experimentally studied in an effort to front this state. They have been classified as endogenous or exogenous antioxidants. Endogenous antioxidants comprise a structure of enzymic enzymatic and non-enzymic enzymatic particles presented within cytoplasm and numerous other subunits in the cells. Exogenous antioxidants include a variety of natural and pharmaceutical agents that may prevent or ameliorate the harmful effects of I/R injury. In this study we review those factors and their ability to enhance the oxidative status of the testis. A feature insight into where we are heading is attempted.

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“…The ischemic/reperfusion state during the twisting of the spermatic cord causes the release of free radicals (Lee et al, 2012), which generally cause damage to DNA and RNA (Nikitaki et al, 2015) and the peroxidation of membrane lipids, triggering the apoptotic signaling pathway (Acevedo-Morantes et al, 2012). However, few studies have evaluated the correlation between urologic diseases, fertility, and genetic damage.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, ischemic organ injury and reperfusion of the contralateral testicle promote histological changes (Jesus, 2000). The damage caused by the reperfusion of the testicle occurs because of oxidative stress via the increased production of reactive oxygen and nitrogen species, which are toxic to cells and lead to membrane lipid peroxidation and apoptosis (Aitken and Roman, 2008;Lee et al, 2012). The combination of these events may cause changes in DNA via disruption of genetic pathways and changes in the cell cycle and cell proliferation, with a consequent increase in apoptotic events (Wei et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Twisting of the spermatic cord: ischemia and reperfusion, toxicogenetic evaluation, and the effects of phosphatidylcholine in pre-clinical trials

Coelho¹,

Berno²,

Falcão

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Phosphatidylcholine is the main phospholipid present in cell membranes and in lipoproteins, and can interfere with various biological processes. This lipid also has antioxidant activity, and protects against damage caused by free radicals under conditions of ischemia/reperfusion. Therefore, the present study was designed to evaluate toxicogenetic damage caused by twisting of the spermatic cord in ischemia/reperfusion, and whether phosphatidylcholine plays a role in conditions of ischemia/reperfusion in preclinical trials. The results indicate that spermatic cord torsion does not cause genotoxic damage or mutagenesis. A dose of 300 mg/kg of phosphatidylcholine is toxic and is thus not recommended. However, a dose of 150 mg/kg does not promote toxicogenetic damage, and though it does not statistically prevent tissue damage occurring from lack of oxygenation and nutrition of testicular cells, it has a tendency to reduce this damage. Therefore, this research suggests that further studies should be conducted to clarify this tendency and to provide a better explanation of the possible therapeutic effects of phosphatidylcholine in cytoprotection of germ cells affected by ischemia/reperfusion.

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Inhaled hydrogen gas therapy for prevention of testicular ischemia/reperfusion injury in rats

Cited by 37 publications

References 40 publications

Protection by Hydrogen Against Gamma Ray‐Induced Testicular Damage in Rats

Protection by Hydrogen Against Gamma Ray‐Induced Testicular Damage in Rats

Antioxidants in experimental ischemia-reperfusion injury of the testis: Where are we heading towards?

Twisting of the spermatic cord: ischemia and reperfusion, toxicogenetic evaluation, and the effects of phosphatidylcholine in pre-clinical trials

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