Inheritance and identification of molecular markers associated with spot blotch (Cochliobolus sativus L.) resistance through microsatellites analysis in barley

Abstract

Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F 1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F 3 , F 4 and F 5 generations. In F 6 and F 6-7 , the quantitative and qualitative approaches also suggested the control of three resistance genes. The parents and the RILs (F 6 /F 6-7 ) were grown in four environments and spot blotch severity recorded. Forty five SSR primers, specific for chromosomes 1 (7H) and 5 (1H), were applied. Of these, 12 were polymorphic between the parents, and between the resistant and susceptible bulks. Three markers BMS 32, BMS 90 and HVCMA showed association with resistance, which was further confirmed through selective genotyping. The co-segregation data on the molecular markers (BMS 32, BMS 90 and HVCMA) and spot blotch severity on 173 RILs was analyzed by single marker linear regression approach. Significant regression suggested linkage among BMS 32, BMS 90 and HVCMA and the three resistant genes (designated as Rcs-qtl-5H-1, Rcs-qtl-5H-2 and Rcs-qtl-1H-1.) respectively. These markers explained 28%, 19% and 12% of variation respectively, for spot blotch resistance among the RILs.

Mapping spot blotch resistance genes in four barley populations

“…Ex Dastur). This major foliar disease occurs in the warmer and more humid growing regions of the world, including North and South America, Europe, Asia, Syria and Australia (Steffenson et al 1996;Kumar et al 2002;Joshi et al 2004;Meldrum et al 2004;Müller et al 2005;Arabi and Jawhar 2007;Kuldeep et al 2008). As the perfect state does not play an important role in the epidemiology of the disease in Australia, the imperfect state is used when referring to the pathogen throughout this paper.…”

Inheritance of spot blotch resistance in barley (Hordeum vulgare L.)

“…is a serious fungal disease with a wide geographical distribution. It is a major disease in warmer and humid growing regions of the world (Kuldeep et al 2008).…”

Validation of microsatellite molecular markers linked with resistance toBipolaris sorokinianain wheat (Triticum aestivumL.)

“…Single marker analysis using regression analysis in SAS version 9.3 (SAS Institute 2011) was done to determine the association between field spot blotch resistance values and the marker genotype data. The band amplified from each marker data was scored as either 0 to indicate absence of the marker or 1 to indicate presence of the marker for regression analysis (Kuldeep et al 2008; Francis et al 2011). Significance of the regression coefficient suggests that there is a relationship between the marker and the trait (Anandhan et al .…”