Inheritance and mapping of vicilin storage protein genes in Pisum Sativum L

Abstract

SUMMARYGel electrophoresis techniques have been used to investigate genetically controlled variation in the major subunits (Mr 50,000) of vicilin, a storage protein of Pisum sativum L. The F1 protein band patterns were shown to be additive with respect to those of the parental lines and to be identical in reciprocal crosses. Genetic analysis of the F2 plants indicated that the 50,000 Mr vicilin subunit band pattern is controlled by a pair of co-dominant genes at single locus. The F2 data were used to locate this major vicilin gene locus (Vc-l) to chromosome 7, closely linked to the r locus (for round and wrinkled seed surface).

Vicilin genes ofPisum

“…3 is drawn from the data of Matta and Gatehouse (1982). 4 The position of Vcl was deduced by Mahmoud and Gatehouse (1984). 5 combines the work presented in this paper with the mapping of a legumin locus detected as a restriction fragment length polymorphism using the legumin cDNA clone pCD43 (Domoney et al 1986) this other class of vicilin mRNA will be difficult.…”

“…6). No vicilin DNA sequence has been found to be tightly linked to the r locus, suggesting that the genes and cDNAs derived from Vc-1 (Mahmoud and Gatehouse 1984) have not been isolated. Furthermore low stringency genomic hybridization experiments, with the probes used here, do not identify fragments unaccounted for in these experiments, suggesting that attempts to isolate Ve-1 Lg-1 Vc-2 RFLP1 Vo-3 Fig.…”

“…For clarity these relationships have been summarized in Table l in which we have extended the nomenclature of Mahmoud and Gatehouse (1984).…”

“…The inheritance of vicilin polypeptides has been studied by , Thomson et al (1980) and Mahmoud and Gatehouse (1984). Thomson and coworkers described variation in apparent molecular masses of the 50K Mr and ca.…”

“…30K Mr polypeptides and in the charge of the 12K Mr and 14K Mr (glycosylated 12K Mr) polypeptides, and showed that these polypeptides behaved as the products of single, codominant Mendelian genes. The work of Mahmoud and Gatehouse (1984) on the major 50 K Mr polypeptides led to the assignment of the locus (Vc-1) for some of the structural genes for this class of polypeptide to a position close to r on chromosome 7.…”