Inheritance and Stability of Somaclonal Variations in Micropropagated Strawberry

Sansavini, S.; Rosati, P.; Gaggioli, D.; Toschi, M. F.

doi:10.17660/actahortic.1990.280.62

Cited by 9 publications

(14 citation statements)

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“…In this experiment, the chimera plants with normal growth was produced in 80 Gy + EMS and two dwarf plants resulted in DNKW001 with 80 Gy and another in Akihime (180 Gy) but died before that produced runner. Similar result was found by Sansavini et al (1990). In this experiment, the dwarf plant loss a chromosome, subsequently it had 55 chromosome (8n-1 or monosomics octoploid).…”

Section: Resultssupporting

confidence: 84%

“…Exploitation of somaclonal variation has been carried out in chrysanthemum (Minano et al, 2009) and strawberry (Popescu et al, 1997;Sansavini et al, 1990;Biswas et al, 2009). The magnitude of somaclonal or abnormal plants depends on concentrations of plant growth regulator (PGR) (Anderson et al, 1982) and good variants were also obtained in strawberry (Biswas et al, 2009) even though in low concentration of PGR.…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry

Murti¹,

Kim²,

Yeoung³

2013

Afr. J. Biotechnol.

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The experiment was conducted to study the effect of gamma-ray irradiation on the high concentration thidiazuron (TDZ) produced buds. In vitro buds were irradiated with different gamma-ray doses. Akihime cultivar ('Akihime') was irradiated with the doses of 0, 30, 80, 130, 180, and 230 Gy while 'DNKW001 accession' ('DNKW001') was exposed to the doses of 0, 30, 80, 130, 180, 230, 280, 300 and 325 Gy and similar doses of gamma rays + EMS 7M (GRE) treatments. Survival rate and plantlet performance of DNKW001 in gamma ray + EMS 7M treatment declined profoundly with increasing doses and LD 50 was lower (104 Gy) than LD 50 in gamma ray irradiation (177 Gy) alone. Variants of plantlets were detected in pre (white streaked leaf and bigger petiole with distorted leaf) and post acclimations (dwarf, dwarf-necrosis, variegated, dark-rigid-thick leaf, rumpled leaf, heart shape-bright red fruit). Hexadecaploid of Akihime and pentadecaploid, 13x + 4 chormosome, and diplodecaploid of DNKW001 were discovered sturdy plants with thicker leaf and bigger pollen than octoploid plant, while the monosomic octoploid performed a dwarf plant. Outstanding variants based on fruit weight, total soluble solid content and color in plot of Principle Component Analysis (PCA) were selected and proved as mutants in DNA level. Gamma ray irradiation + EMS was more effective to generate more type and magnitude of variants. Irradiation dose less than 130 Gy was ample for generating variant plants of strawberry.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry

Murti¹,

Kim²,

Yeoung³

2013

Afr. J. Biotechnol.

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“…According to Sansavini et al [1990] and Kaushal et al [2004], strawberry clonal propagation on large scale, while plant multiplying is maximized by using high concentration of PGRs, may induce high rate of somaclonal variation [Sansavini et al 1990, Kaushal et al 2004, which is a huge problem in production of genotypic and phenotypic uniform plants. Among these changes -leaf colour, occurring of dwarf plants and many others stands out [Cameron and Hancock 1986, Sansavini et al 1990, Irkaeva and Matveena 1997.…”

Section: Genetic Stabilitymentioning

confidence: 99%

“…Among these changes -leaf colour, occurring of dwarf plants and many others stands out [Cameron and Hancock 1986, Sansavini et al 1990, Irkaeva and Matveena 1997. Modified traits may be epigenetic and therefore they can disappear with time [Koruza and Jelaska 1993].…”

Section: Genetic Stabilitymentioning

confidence: 99%

In vitro REGENERATION OF Fragraria PLANTS

Rokosa

Mikiciuk

2017

Acta Sci. Pol. Hortorum Cultus

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Fragaria is one of genus in Rosaceae family. The most popular representative is strawberry (Fragaria × ananassa Duch.) and wild (woodland) strawberry (Fragaria vesca L.), which taste attributes are very attractive for a huge number of consumers around the world. The plants have many beneficial traits, such as low-caloric, high amount of antoxidants and vitamin C, laxative, diuretic, astringent, antidiarrheal and antiseptic properties. Cultivation of Fragaria plants is widespread worldwide with particular emphasis on moderate climate zone, also with use of a plant tissue culture method. This thesis showed and contrasted other studies about Fragaria plants propagation under in vitro conditions. In this method the most often used explants are leaf explants. Very rarely are used seeds. Mainly, the plants are propagated on basal medium of mineral composition by Murashige and Skoog in 8 hour dark and 16 hour light contidions. The most efficient cytokinin used to root induction is indole-3-butyric acid (IBA). The plant acclimatization had varying effectiveness -from a few to several dozen survival percent. During micropropagation of Fragaria plants, somaclonal variation occurs, which is dependent on age culture, frequency of passage and medium composition.

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“…Somaclonal variation may generate traits that are not present in the ge- Sansavini et al (1990) reported that somaclonal variations occurring during micropropagation of strawberry were inherited during the first propagation from runners but subsequently declined in the next seasons. Similarly, Biswas et al (2009) found that some of the somaclonal traits elicited in strawberry in vitro cultures reverted back to their original phenotype within 2-3 vegetative generations.…”

mentioning

confidence: 99%

Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones

Sowik¹,

Markiewicz²,

Michalczuk³

2015

Hort. Sci. (Prague)

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Sowik I., Markiewicz M., Michalczuk L. (2015): Stability of Verticillium dahliae resistance in tissue culturederived strawberry somaclones. Hort. Sci. (Prague), 42: 141-148.Somaclonal variation is a novel source of useful traits in strawberry breeding. In our previous work we selected K40 clone from in vitro cultures of strawberry cv. Elsanta, which was characterized by high resistance to Verticillium dahliae. The objective of this work was to evaluate the stability and transmission of this trait during in vitro shoot proliferation and traditional clonal propagation from runners. K40, Elsanta and Senga Sengana plants were propagated in vitro for 45 generations/passages or for 4 generation of clonal propagation from runners. The resistance/susceptibility of such derived plants to V. dahliae was tested in controlled conditions in a greenhouse. Irrespective of the method of propagation, the plants of K40 clone were much more resistant to V. dahliae than the plants of the original cultivar and the plants of 'Senga Sengana' , which is a cultivar considered as resistant to Verticillium wilt in field conditions. This proves that this trait elicited at somaclonal variant is stable and transferable both during in vitro micropropagation and clonal plant propagation from runners.

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Inheritance and Stability of Somaclonal Variations in Micropropagated Strawberry

Cited by 9 publications

References 0 publications

Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry

Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry

In vitro REGENERATION OF Fragraria PLANTS

Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones

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