Inheritance of RAPD molecular makers in lake trout Salvelinus namaycush

Abstract

Inheritance patterns of randomly amplified polymorphic DNA (RAPD) phenotypes were tested in 13 full-sib families of lake trout Salvelinus namaycush. Single-pair matings of parents with known phenotypes were made, and up to 20 progeny of each mating were used to test inheritance patterns. Seven RAPD primers amplified 13 polymorphic bands. With the exception of one family, expected segregation ratios for dominant Mendelian genetic traits were observed. Our results support previously reported findings that RAPD markers can be considered Mendelian traits and therefore could be used for analysis of genetic population structure.

Inheritance of molecular markers and sex in the Australian freshwater crayfish, Cherax destructor Clark

“…In addition, non‐parental bands were not detected in any offspring of C. destructor . This is in contrast to findings in several other species (Reidy, Hamilton & Aquadro 1992; Reiter, Williams, Feldmann, Rafalski, Tingey & Scolnik 1992; Garcia & Benzie 1995; Stott et al 1997), which represent a major drawback for the use of RAPD markers for population genetic and diagnostic purposes. In this study, three independent DNA extracts from each sample were used to test for reproducibility of RAPD bands and only bands that were present in all three replicates were scored.…”

“…However, direct determination of genotypes could be inferred for the F 2 families. The RAPD markers exhibited Mendelian inheritance patterns consistent with a dominant marker system as has been reported for other aquatic organisms (Levitan & Groseberg 1993;Johnson, Midson, Ballinger & Postlethwaite 1994;Foo, Dinesh, Lim, Chan & Phang 1995;Garcia & Benzie 1995;Stott, Ihsen & White 1997;Liu, Li, Argue & Dunham 1999;Appleyard & Mather 2000). In addition, non-parental bands were not detected in any o¡spring of C. destructor.…”

Inheritance of amplified fragment length polymorphism markers and their utility in population genetic analysis of Plecoglossus altivelis

“…Although one unexpected nonparental fragment was observed from amplified fragment patterns of 140 larvae, it was considered to have resulted from a mutation or experimental artifacts. The frequency of the occurrence of such a fragment (0Á7% of individuals) was equivalent to or much lower than that of nonparental bands in RAPD analysis (Scott et al, 1997), so it would hardly affect the results of applications of the AFLP method to biological investigations, such as population genetic analyses.…”

Evidence of Sibling Species in the Brown Planthopper Complex (Nilaparvata lugens) Detected from Short and Long Primer Random Amplified Polymorphic DNA Fingerprints

“…Nonparental bands were also observed in the current study very rarely. A drawback of some RAPD markers has been the observed lack of heritability of amplified products and presence of nonparental RAPD bands in the offspring (Riedy et al 1992;Stott et al 1997). The dendrogram from cluster analysis and the results of principal coordinate analysis from SP and LP-RAPD data revealed the genetic relationships among insects of different populations with high and low esterase activity.…”