Inheritance of Reduced Palmitic Acid Content in Seed Oil of Soybean

Fehr, W. R.; Welke, Grace A.; Hammond, Earl G.; Duvick, Donald N.; Cianzio, Silvia R.

doi:10.2135/cropsci1991.0011183x003100010022x

Cited by 85 publications

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“…B019 was a line with NP and reduced palmitate developed jointly by Iowa State University and Pioneer Hi-Bred International, Inc. The reduced-palmitate trait in B019 was controlled by the recessive alleles/api and fap 3 (Fehr et al, 1991). A total of 20 BCiF 2 -derived lines that had LP and reduced-palmitate and 20 lines that had NP and reduced-palmitate were evaluated at three Iowa locations in 2003.…”

Section: Influence Of Low Phytate On Agronomic and Seed Traitsmentioning

confidence: 99%

“…CXI834, the donor of the phal and pha2 alleles, was obtained from the USDA-ARS and Purdue University. B019 was a line homozygous for ihefapl and fap3 alleles for reduced palmitate that was developed jointly by Iowa State University and Pioneer Hi-Bred International, Inc (Fehr et al, 1991 The desired genotype was Phal phal Pha2pha2 because it was the only one that could produce LP BCiF 2 progeny. Eleven seeds from each BCIFI plant were analyzed to identify those that had at least one LP seed, which indicated that the plant had the Phal phal Pha2pha2 genotype.…”

Section: Line Developmentmentioning

confidence: 99%

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Genetic Improvement of Seedling Emergence of Soybean Lines with Low Phytate

Spear

Fehr

2007

Crop Science

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Seedling emergence of low‐phytate (LP) soybean [Glycine max (L.) Merr.] lines has been reported to be lower than that of normal‐phytate (NP) lines. One objective of this study was to evaluate if backcrossing the LP trait into a NP line would result in LP progeny with normal emergence. The LP line CX1834‐1‐6 (CX1834) was crossed to B01769B019 (B019), a NP line with reduced palmitate content, and three backcrosses were made to B019. A total of 36 BC3F4–derived LP lines from the population were evaluated at five locations in 2005 in comparison with CX1834, B019, and an NP cultivar IA3023. The mean phytate P and inorganic P content of all the backcross lines was not significantly different from CX1834. There were 18 backcross lines that had a mean field emergence that was significantly greater than CX1834 and not significantly different from B019. The results indicated that backcrossing seemed to be successful for developing LP lines with normal field emergence. A second objective of the study was to determine the effectiveness of warm germination, cold vigor, and accelerated aging tests for predicting field emergence of LP lines. Fifteen of the backcross lines were evaluated in the three tests that represented the range of field emergence that had been observed. The tests were useful for identifying lines with inferior field emergence but were not reliable enough to replace field tests for identifying the best emerging lines.

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Section: Influence Of Low Phytate On Agronomic and Seed Traitsmentioning

confidence: 99%

Section: Line Developmentmentioning

confidence: 99%

Genetic Improvement of Seedling Emergence of Soybean Lines with Low Phytate

Spear

Fehr

2007

Crop Science

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“…Several lines with reduced palmitic acid concentration were developed by ethyl-methyl sulfonate mutagenesis (C1726, homozygous for the fap1 allele and ELLP2, homozygous for the fap * or fapx alleles); N-nitroso-Nmethyl urea mutagenesis (A22, homozygous for the fap3 allele); and X-radiation (J3 homozygous for the sop1 allele) (Fehr et al 1991;Stojšin et al 1998;Wilcox and Cavins 1990;Rahman et al 1996). In addition, one natural mutation (fap nc ) was discovered in line N79-2077-11 and its sister line N79-2077-12, both derived from the fifth cycle of a recurrent selection program for high oleic concentration (Burton et al 1983;Burton et al 1994b).…”

Section: Introductionmentioning

confidence: 99%

Mapping the low palmitate fap1 mutation and validation of its effects in soybean oil and agronomic traits in three soybean populations

et al. 2013

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Key message fap1 mutation is caused by a G174A change in GmKASIIIA that disrupts a donor splice site recognition and creates a GATCTG motif that enhanced its expression. Abstract Soybean oil with reduced palmitic acid content is desirable to reduce the health risks associated with consumption of this fatty acid. The objectives of this study were: to identify the genomic location of the reduced palmitate fap1 mutation, determine its molecular basis, estimate the amount of phenotypic variation in fatty acid composition explained by this locus, determine if there are epistatic interactions between the fap1 and fap nc loci and, determine if the fap1 mutation has pleiotropic effects on seed yield, oil and protein content in three soybean populations. This study detected two major QTL for 16:0 content located in chromosome 5 (GmFATB1a, fap nc ) and chromosome 9 near BARCSOYSSR_09_1707 that explained, with their interaction, 66-94 % of the variation in 16:0 content in the three populations. Sequencing results of a putative candidate gene, GmKASIIIA, revealed a single unique polymorphism in the germplasm line C1726, which was predicted to disrupt the donor splice site recognition between exon one and intron one and produce a truncated KASIIIA protein. This G to A change also created the GATCTG motif that enhanced gene expression of the mutated GmKASIIIA gene. Lines homozygous for the GmKASIIIA mutation (fap1) had a significant reduction in 16:0, 18:0, and oil content; and an increase in unsaturated fatty acids content. There were significant epistatic interactions between GmKASIIIA (fap1) and fap nc for 16:0 and oil contents, and seed yield in two populations. In conclusion, the fap1 phenotype is caused by a single unique SNP in the GmKASIIIA gene.

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“…The fapl and fap3 alleles were independently developed by treatment of seeds of commercial soybean cultivars with chemical mutagens (Erickson et al, 1988;Fehr et al, 1991). Genotypes homozygous for the two alleles have about 40 g kg-1…”

mentioning

confidence: 99%

Influence of Reduced Palmitate Content on Agronomic and Seed Traits of Soybean

Ndzana¹,

Fehr²,

Welke

et al. 1994

Crop Science

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Soybean [Glycine max (L.) Merr.]genotypes with reduced palmitate content have been developed to improve the nutritional characteristics of the seed oil. The objective of the study was to determine the influence of reduced palmitate on agronomic and seed traits of sobean. Three lines homozygous for the fap1 fap3 alleles (≈ 40g kg−1 palmitate) were crossed to different high‐yielding cultivars (≈110 g kg−1 palmitate). In total, 27 F4‐derived lines with reduced palmitate and 27 lines with normal palmitate content were selected from each of the three single‐cross populations. The lines and parents of each population were grown in separate replicated field tests at three locations in Iowa during 1992. The mean seed yield of the reduced‐palmitate lines was significantly less than that of the normal‐palmitate lines was significantly less than that of the normal‐palmitate lines in two of the three populations. The reduced‐palmitate lines had significantly greater protein content in two crosses and significantly lower oil content than the normal‐palmitate lines in all crosses. The associations of reduced palmitate with agronomic and seed traits could be attributable to pleiotropic effects of the fap1 or fap3 alleles or to possible linkages of the alleles with genes controlling the other traits.

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Inheritance of Reduced Palmitic Acid Content in Seed Oil of Soybean

Cited by 85 publications

References 0 publications

Genetic Improvement of Seedling Emergence of Soybean Lines with Low Phytate

Genetic Improvement of Seedling Emergence of Soybean Lines with Low Phytate

Mapping the low palmitate fap1 mutation and validation of its effects in soybean oil and agronomic traits in three soybean populations

Influence of Reduced Palmitate Content on Agronomic and Seed Traits of Soybean

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