Inheritance of self- and graft-incompatibility traits in an F1 apricot progeny

Irisarri, Patricia; Zhebentyayeva, Tetyana; Errea, P.; Pina, Ana

doi:10.1371/journal.pone.0216371

Cited by 17 publications

(13 citation statements)

References 51 publications

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“…To generate a hybrid plant material for genetic analysis, we cross-pollinated two apricot cultivars that were previously phenotyped as graft-compatible and -incompatible when grafted to the same rootstock universal for Prunus. Using this cross, we established a phenotyping protocol for this complex trait based on cytomorphological observations of graft interface (Irisarri et al, 2019). Regression analysis of phenotypic data across the progeny revealed likely polygenic control of successful graft formation.…”

Section: Introductionmentioning

confidence: 99%

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

et al. 2021

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Graft incompatibility (GI) between the most popular Prunus rootstocks and apricot cultivars is one of the major problems for rootstock usage and improvement. Failure in producing long-leaving healthy grafts greatly affects the range of available Prunus rootstocks for apricot cultivation. Despite recent advances related to the molecular mechanisms of a graft-union formation between rootstock and scion, information on genetic control of this trait in woody plants is essentially missing because of a lack of hybrid crosses, segregating for the trait. In this study, we have employed the next-generation sequencing technology to generate the single-nucleotide polymorphism (SNP) markers and construct parental linkage maps for an apricot F1 population “Moniqui (Mo)” × “Paviot (Pa)” segregating for ability to form successful grafts with universal Prunus rootstock “Marianna 2624”. To localize genomic regions associated with this trait, we genotyped 138 individuals from the “Mo × Pa” cross and constructed medium-saturated genetic maps. The female “Mo” and male “Pa” maps were composed of 557 and 501 SNPs and organized in eight linkage groups that covered 780.2 and 690.4 cM of genetic distance, respectively. Parental maps were aligned to the Prunus persica v2.0 genome and revealed a high colinearity with the Prunus reference map. Two-year phenotypic data for characters associated with unsuccessful grafting such as necrotic line (NL), bark and wood discontinuities (BD and WD), and an overall estimate of graft (in)compatibility (GI) were collected for mapping quantitative trait loci (QTLs) on both parental maps. On the map of the graft-compatible parent “Pa”, two genomic regions on LG5 (44.9–60.8 cM) and LG8 (33.2–39.2 cM) were associated with graft (in)compatibility characters at different significance level, depending on phenotypic dataset. Of these, the LG8 QTL interval was most consistent between the years and supported by two significant and two putative QTLs. To our best knowledge, this is the first report on QTLs for graft (in)compatibility in woody plants. Results of this work will provide a valuable genomic resource for apricot breeding programs and facilitate future efforts focused on candidate genes discovery for graft (in)compatibility in apricot and other Prunus species.

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Section: Introductionmentioning

confidence: 99%

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

et al. 2021

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“…However, phenotyping graft compatibility in large populations is challenging as it requires grafting many (hundreds) individuals to accurately score this trait, which has many logistical problems. To begin to overcome these problems, recently graft union formation was assessed at 1 month and 1 year after grafting by scoring the necrotic line, and wood and bark discontinuity, and cellular arrangements at the interface in a bi-parental F1 apricot scion population grafted onto a plum rootstock (Irisarri et al, 2019 ). Continuous variation was found in the graft union traits scored (Irisarri et al, 2019 ); this study paves the way for further studies into the genetic control of graft union formation.…”

Section: Discussionmentioning

confidence: 99%

“…To begin to overcome these problems, recently graft union formation was assessed at 1 month and 1 year after grafting by scoring the necrotic line, and wood and bark discontinuity, and cellular arrangements at the interface in a bi-parental F1 apricot scion population grafted onto a plum rootstock (Irisarri et al, 2019 ). Continuous variation was found in the graft union traits scored (Irisarri et al, 2019 ); this study paves the way for further studies into the genetic control of graft union formation. However, understanding the genetic basis of graft incompatibility would be greatly accelerated if marker metabolites or transcripts could be identified.…”

Section: Discussionmentioning

confidence: 99%

Identifying Molecular Markers of Successful Graft Union Formation and Compatibility

Loupit

Cookson

2020

Front. Plant Sci.

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Grafting is a technique used for millennia for vegetative propagation, especially in perennial fruit crops. This method, used on woody and herbaceous plants, can improve several agronomic characteristics, such as yield or vigor, as well as tolerance to biotic and abiotic stresses. However, some scion/rootstock combinations suffer from poor graft compatibility, i.e., they are unable to form and/or sustain a successful graft union. Identifying symptoms of graft incompatibility is difficult because they are not always present in the first years after grafting and in most cases the causes of incompatibility are still poorly understood. Studies of changes in transcript abundance during graft union formation indicate that grafting responses are similar to responses to wounding and include the differential expression of genes related to hormone signaling, oxidative stress, formation of new vascular vessels, cell development, and secondary metabolites, in particular polyphenols. This review summarizes current knowledge of the changes in transcript abundance, redox status and metabolites accumulation during graft union formation and in cases of graft incompatibility. The goal of this review is to discuss the possibility of identifying marker transcripts, enzyme activities and/or metabolites of grafting success and graft compatibility which could be used to score grafting success for genetic research and in breeding programs. We highlight gaps in current knowledge and potential research directions in this field.

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“…Interestingly, these grape receptor kinase genes included homologs of S-locus glycoprotein-like gene family. Nevertheless it is important to note that in spite of these general similarities Irisarri et al (2019) found no correlation between GSI and graft compatibility in the analysis of an apricot (Prunus armeniaca) F 1 segregating population.…”

Section: Si and Other Self/non-self Discrimination Systemsmentioning

confidence: 93%

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

et al. 2020

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Self-incompatibility (SI) mechanisms prevent self-fertilization in flowering plants based on specific discrimination between self-and non-self pollen. Since this trait promotes outcrossing and avoids inbreeding it is a widespread mechanism of controlling sexual plant reproduction. Growers and breeders have effectively exploited SI as a tool for manipulating domesticated crops for thousands of years. However, only within the past thirty years have studies begun to elucidate the underlying molecular features of SI. The specific S-determinants and some modifier factors controlling SI have been identified in the sporophytic system exhibited by Brassica species and in the two very distinct gametophytic systems present in Papaveraceae on one side and in Solanaceae, Rosaceae, and Plantaginaceae on the other. Molecular level studies have enabled SI to SC transitions (and vice versa) to be intentionally manipulated using marker assisted breeding and targeted approaches based on transgene integration, silencing, and more recently CRISPR knock-out of SI-related factors. These scientific advances have, in turn, provided a solid basis to implement new crop production and plant breeding practices. Applications of self-(in)compatibility include widely differing objectives such as crop yield and quality improvement, marker-assisted breeding through SI genotyping, and development of hybrids for overcoming intra-and interspecific reproductive barriers. Here, we review scientific progress as well as patented applications of SI, and also highlight future prospects including further elucidation of SI systems, deepening our understanding of SI-environment relationships, and new perspectives on plant self/non-self recognition.

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Inheritance of self- and graft-incompatibility traits in an F1 apricot progeny

Cited by 17 publications

References 51 publications

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

Mapping Quantitative Trait Loci Associated With Graft (In)Compatibility in Apricot (Prunus armeniaca L.)

Identifying Molecular Markers of Successful Graft Union Formation and Compatibility

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

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