Inheritance Pattern and Molecular Markers for Resistance to Blackleg Disease in Cabbage

Abstract

The inheritance and causal loci for resistance to blackleg, a devastating disease of Brassicaceous crops, are yet to be known in cabbage (Brassica oleracea L.). Here, we report the pattern of inheritance and linked molecular marker for this trait. A segregating BC1 population consisting of 253 plants was raised from resistant and susceptible parents, L29 (♀) and L16 (♂), respectively. Cotyledon resistance bioassay of BC1 population, measured based on a scale of 0–9 at 12 days after inoculation with Leptosphaeria maculans isolate 03–02 s, revealed the segregation of resistance and ratio, indicative of dominant monogenic control of the trait. Investigation of potential polymorphism in the previously identified differentially expressed genes within the collinear region of ‘B. napus blackleg resistant loci Rlm1′ in B. oleracea identified two insertion/deletion (InDel) mutations in the intron and numerous single nucleotide polymorphisms (SNPs) throughout the LRR-RLK gene Bol040029, of which six SNPs in the first exon caused the loss of two LRR domains in the susceptible line. An InDel marker, BLR-C-InDel based on the InDel mutations, and a high resolution melting (HRM) marker, BLR-C-2808 based on the SNP C2808T in the second exon were developed, which predicated the resistance status of the BC1 population with 80.24%, and of 24 commercial inbred lines with 100% detection accuracy. This is the first report of inheritance and molecular markers linked with blackleg resistance in cabbage. This study will enhance our understanding of the trait, and will be helpful in marker assisted breeding aiming at developing resistant cabbage varieties.

Mining of Cloned Disease Resistance Gene Homologs (CDRHs) in Brassica Species and Arabidopsis thaliana

“…Nevertheless, truncated RGAs such as NL and TN have been cloned and functionally characterised with disease resistance in A. thaliana [ 53 , 61 , 62 ]. While a NBS gene has been reported as a signalling component in disease resistance [ 20 ], genes with TX domains were able to interact with different R and Avr genes to elicit disease resistance in A. thaliana [ 169 , 170 ], and CC domain has been reported as a candidate for the blackleg R genes Rlm1, LepR2, and LepR4 in B. oleracea [ 171 , 172 , 173 ].…”

In silico prediction and analysis of transmembrane‐coiled‐coil resistance gene analogues in 27Brassicaceaespecies

“… a 1, Stotz et al (2018); 2, Inturrisi et al (2022); 3, Lee et al (2015); 4, Ferdous, Hossain, Park, Kim, et al (2020); 5, Hossain et al (2020); 6, Ferdous, Hossain, Park, Robin, et al (2020); 7, Ferdous et al (2019); 8, Chang et al (2019); 9, Yang et al (2021); 10, Raman et al (2021). …”

Improving the Selection Efficiency of Breeding Material within Interspecific Brassicaceae Hybrids with Genomic Prediction and Phenotyping

“…As mentioned earlier, the markers Bol023847, Bol040029, Bol040038, Bol040045, and Bol040099 are associated with the region of the A genome, containing many blackleg resistance genes. In the study by Ferdous et al [29], the use of four of the aforementioned markers (Bol023847, Bol040038, Bol040045, and Bol040099) failed to reveal polymorphisms in the amplified DNA region. However, during the course of the present analyses, a reaction product of a different size than expected was observed for the marker Bol040045.…”