Inhibin B as a Marker of Sertoli Cell Damage and Spermatogenic Disturbance in the Rat

Pfaff, Tamara; Rhodes, Jon; Bergmann, Martin; Weinbauer, Gerhard F.

doi:10.1002/bdrb.21046

Cited by 17 publications

(17 citation statements)

References 23 publications

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“…(i) Inhibin B can decrease after sufficient damage. This is best illustrated by several figures in the companion articles: Figure 6 in Coulson et al (), Figures 6 and 7 in Sonee et al (), Figure in Enright et al (), Figures and in Breslin et al (), and Figure 7 in Pfaff et al (). The Chapin et al (), Coulson et al (), Sonee et al (), Erdos et al (), and Pfaff et al () articles gave graded pathology scores, and plotted circulating Inhibin B values against these scores, nicely revealing a monotonic decrease but often driven mostly by the reductions only at severe levels of germ cell loss and tissue degeneration.…”

Section: Combined Resultsmentioning

confidence: 93%

“…We did not determine the specificity of the protein (i.e., that it comes only from the testis). The pathogenesis correlation studies are reported in the nine preceding articles in this issue of Birth Defects Research (Breslin et al, ; Chapin et al, ; Coulson et al, ; Enright et al, ; Erdos et al, ; Moffit et al, ; Pfaff et al, ; Sonee et al, ; Ziejewski et al, ). The present paper is intended to review and discuss the data presented by each of these preceding reports.…”

Section: Introductionmentioning

confidence: 99%

“…Generally, the studies varied by route of administration and duration of treatment, but all were conducted in the rat and assessed testicular pathology and Inhibin B levels in serum or plasma. One study (Pfaff et al, ) used a different analytical Inhibin B assay, which is no longer commercially available.…”

Section: Introductionmentioning

confidence: 99%

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Summary of the HESI Consortium Studies Exploring Circulating Inhibin B as a Potential Biomarker of Testis Damage in the Rat

Chapin

Weinbauer

Thibodeau

et al. 2013

Birth Defects Research Pt B

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The Developmental and Reproductive Toxicity Technical Committee of the Health and Environmental Sciences Institute hosted a working consortium of companies to evaluate a new commercially available analytic assay for Inhibin B in rat serum or plasma. After demonstrating that the kit was stable and robust, the group performed a series of independent pathogenesis studies (23 different compound/investigator combinations) designed to examine the correlation between the appearance of lesions in the testis and changes in circulating levels of Inhibin B. These studies were reported individually in the previous articles in this series (this issue), and are discussed in this paper. For roughly half of these exposures, lesions appeared well before Inhibin B changed. A few of the studies showed a good correlation between seminiferous tubule damage and reduced circulating Inhibin B levels, while for seven exposures, circulating Inhibin B was reduced with no detectable alteration in testis histology. Whether this indicates a prodromal response or a false-positive signal will require further investigation. These exceptions could plausibly suggest some value of circulating Inhibin B as a useful biomarker in some circumstances. However, for roughly half of these exposures, Inhibin B appeared to be a lagging biomarker, requiring significant damage to the seminiferous tubules before a consistent and credible reduction in circulating levels of Inhibin B was observed.

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Section: Combined Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Summary of the HESI Consortium Studies Exploring Circulating Inhibin B as a Potential Biomarker of Testis Damage in the Rat

Chapin

Weinbauer

Thibodeau

et al. 2013

Birth Defects Research Pt B

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“…Inhibin B is an important hormone secreted by SCs, and it is considered a marker of Sertoli cell damage and spermatogenic disturbance (Pfaff et al. ). As shown above, CEBP β significantly affects the release of inhibin B.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of C/EBPβ Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells

Tang

Jin

Chen

et al. 2016

Reprod Domestic Animals

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CCAAT/enhancer-binding protein beta (C/EBPβ), an important transcriptional factor, plays a pivotal role in the regulation of female germ cell development. However, the role of C/EBPβ on the development of male germ cells has not been reported. In this study, we constructed the recombinant adenovirus plasmids of bovine C/EBPβ gene and harvested the subsequent adenoviruses, and then assessed the mRNA levels of spermatogenesis-related genes (real-time PCR) and secretion of inhibin B after 48 h of Ad-C/EBPβ recombinant adenovirus infection in bovine sertoli cells (SCs). We found that overexpression of exogenous C/EBPβ gene upregulated the mRNA expressions of spermatogenesis-related genes, including Pdgfa, Claudin, Caspase-3, Occludin, kit1 and Cyclin E, and decreased the mRNA levels of Cyclin D1. Meanwhile, overexpression of exogenous C/EBPβ gene significantly increases the amounts of secreted inhibin B. In conclusion, the results indicate that the C/EBPβ gene plays an important regulatory role in regulation of the cell cycle regulators and spermatogenesis-related genes expression and function of bovine SCs.

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“…(2) Stress exposure can increase the concentration of cortisol which can disturb the testis by reduction of Leydig cell numbers and inducing apoptosis by glucocorticoid receptors (3) and also by reducing the quality of Sertoli cells which can decrease the production of germinal cells, depress spermatogenesis, and finally can cause infertility. (4) High glucocorticoid concentrations during the stress period can induce Leydig cell apoptosis by various mechanisms, such as activation of the Fas system, cleavage of procapase-3, removal of mitochondrial membrane potential (DeltaPsi) and elevation of ROS concentration. (3) One of the apoptosis markers is the elevated expression of caspase-3 (the active form of procaspase-3).…”

Section: Introductionmentioning

confidence: 99%

Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats

Arjadi

Partadireja

Maurits

et al. 2015

UnivMed

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BACKGROUNDSleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) . METHODSThis experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method. RESULTSMean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups. CONCLUSIONSleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes. Keywords INTRODUCTIONAt the molecular level, male infertility occurs because of abnormal mitochondrial deoxyribonucleic acid (DNA) and apoptosis mechanism.(1) When apoptosis is improperly activated or regulated in the testis, infertility can result. Pinpointing how environmental conditions can affect apoptosis is important for the advancement of preventive medicine and behavioral science, especially as there are potentially harmful environments in workplaces. Moreover, familiarity with stressful conditions in workplaces and with testicular processes, especially the induction of apoptosis, is essential for promoting male fertility. In male patients with idiopathic infertility, reactive oxygen species (ROS)-induced sperm damage is associated with increased apoptosis. (2) Stress exposure can increase the concentration of cortisol which can disturb the testis by reduction of Leydig cell numbers and 89 Univ Med Vol. 34 No.2

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Inhibin B as a Marker of Sertoli Cell Damage and Spermatogenic Disturbance in the Rat

Cited by 17 publications

References 23 publications

Summary of the HESI Consortium Studies Exploring Circulating Inhibin B as a Potential Biomarker of Testis Damage in the Rat

Summary of the HESI Consortium Studies Exploring Circulating Inhibin B as a Potential Biomarker of Testis Damage in the Rat

Overexpression of C/EBPβ Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells

Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats

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