INHIBITION AND ENHANCEMENT OF SINGLET OXYGEN (‘A<sub>g</sub>) DIMOL CHEMILUMINESCENCE

Deneke, Carl F.; Krinsky, Norman I.

doi:10.1111/j.1751-1097.1977.tb06914.x

Cited by 72 publications

(44 citation statements)

References 22 publications

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“…10 " 15 The results obtained using this new technique were found to be in agreement with the free radical reaction as determined simultaneously from the perspective of energy metabolism.…”

supporting

confidence: 58%

“…The spectroanalysis reveals the luminous mechanism as follows: 'Ag + 'Ag -» 2 3 O 2 + h/x Stroke Vol 15, No 6, 1984 IN RECENT YEARS THE AUTO-OXIDATION of unsaturated fatty acids has been suggested as one of the primary factors in the acute stage pathology of the ischemic or hypoxic brain.'" 4 It has been difficult to detect the free radical reactions and further uncertainties remain with regard to the correlation between the period during which lipid peroxidation occurs and the energy metabolism of the brain.5 " 9In the present study, we have followed the sequential changes of chemiluminescence and energy metabolism during a period of both induced hypoxia and oxygen resupply by using supratentorial tissue of hypoxic rat brain (PaO 2 , 17-22 mmHg).Chemiluminescence analysis is a new technique in which the propagation or termination of free radical reaction is thought to appear when singlet oxygen has been transmitted to the triplet oxygen.10 " 15 The results obtained using this new technique were found to be in agreement with the free radical reaction as determined simultaneously from the perspective of energy metabolism. …”

mentioning

confidence: 99%

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Chemiluminescence in hypoxic brain--the first report. Correlation between energy metabolism and free radical reaction.

Imaizumi¹,

Kayama²,

Suzuki³

1984

Stroke

102

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SUMMARY The possibility that cerebral ischemia or cerebral hypoxia may initiate a series of free radical reactions in brain tissue lipid constituents was explored by measuring sequential changes in chemiluminescence values and energy metabolism during brain hypoxia in the rat. Brain hypoxia was induced by means of arterial hypoxemia (PaO 2 17-22 mmHg), normocapnia (PaCO 2 28-38 mmHg) and normotension (MABP 100-140 mmHg). To obtain lowered PaO 2 , 4% O 2 -96% N 2 mixed gas was used. Analysis of the chemiluminescence spectra for the purpose of luminous mechanism investigation was again attempted. No peroxidation occurred in the pre-hypoxic state since there were no photon counts. Chemiluminescence began to rise in the hypoxic state and remained at a high value in the post-hypoxic state. Specifically in the hypoxic state, the 3 min period showed 231 ± 35 counts/10 sec-g (n = 5) and the 5 min period showed 154 ± 62 (n = 19) counts/10 sec-g. In the post-hypoxic state, the 5 min period showed 217 ± 79 counts/10 sec-g (n = 9) and the 30 min period showed a decrease similar to the pre-hypoxlc state. The chemiluminescence spectroanaljsis showed five peaks in wavelength at 480 nm, 520-530 nm, 570 nm, 620-640 nm and 680-700 nm. Sequential changes in energy metabolism revealed that hypoxia caused marked brain lactic acidosis, an increase in both ADP and pyruvate, and a fall in glucose. However, all metabolites recovered at 30 min in the post-hypoxic state, which suggests this was reversible brain hypoxia. Sequential changes in chemiluminescence values and energy metabolism imply the occurrence of free radical reaction in the hypoxic and posthypoxic brain. The spectroanalysis reveals the luminous mechanism as follows: 'Ag + 'Ag -» 2 3 O 2 + h/x Stroke Vol 15, No 6, 1984 IN RECENT YEARS THE AUTO-OXIDATION of unsaturated fatty acids has been suggested as one of the primary factors in the acute stage pathology of the ischemic or hypoxic brain.'" 4 It has been difficult to detect the free radical reactions and further uncertainties remain with regard to the correlation between the period during which lipid peroxidation occurs and the energy metabolism of the brain.5 " 9In the present study, we have followed the sequential changes of chemiluminescence and energy metabolism during a period of both induced hypoxia and oxygen resupply by using supratentorial tissue of hypoxic rat brain (PaO 2 , 17-22 mmHg).Chemiluminescence analysis is a new technique in which the propagation or termination of free radical reaction is thought to appear when singlet oxygen has been transmitted to the triplet oxygen.10 " 15 The results obtained using this new technique were found to be in agreement with the free radical reaction as determined simultaneously from the perspective of energy metabolism. Experimental Materials and MethodsFor the purpose of this study, 142 male Wister rats (250-280 g) were used. In the chemiluminescence value study, 9 rats were sacrificed at pre-hypoxia, 5 at hypoxia-3 min, 19 at hypoxia-5 min, 9 at post-hypoxia-5 min, a...

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“…10 " 15 The results obtained using this new technique were found to be in agreement with the free radical reaction as determined simultaneously from the perspective of energy metabolism.…”

supporting

confidence: 58%

mentioning

confidence: 99%

Chemiluminescence in hypoxic brain--the first report. Correlation between energy metabolism and free radical reaction.

Imaizumi¹,

Kayama²,

Suzuki³

1984

Stroke

102

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“…During these reactions a chemiluminescence can be generated with involvement of singlet oxygen as an intermediate (36). The inhibition of native chemiluminescence by sodium azide and glutathione in our experiments can be explained by their action as scavengers of singlet oxygen (37) or antioxidant, respectively.…”

Section: Discussionmentioning

confidence: 84%

Increased native chemiluminescence in granulocytes isolated from synovial fluid and peripheral blood of patients with rheumatoid arthritis

Arnhold

Sonntag

Sauer

et al. 1994

J. Biolumin. Chemilumin.

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Polymorphonuclear leukocytes (PMNs) isolated from peripheral blood and synovial fluid of patients with rheumatoid arthritis and from peripheral blood of volunteers were stimulated with 12-phorbol-13-myristate acetate (PMA). No significant differences in luminol-amplified chemiluminescence were found between different patients and control groups. However, two distinct patterns of native chemiluminescence were observed. Type I showed no, or only a small, increase in native chemiluminescence with integral counts over 30 min less than 3 x 10(5) cpm, and the majority of samples from volunteers were of this type. Type II was characterized by a burst of native chemiluminescence starting 8 to 15 min after cell stimulation. It was found in most PMN samples from patients with rheumatoid arthritis. Integral counts over 30 min were always higher than 10(6) cpm and as high as 10(8) cpm in some cases. A strong inhibition of the Type II native chemiluminescence was caused by desferal, catalase, thiourea, and glutathione. However, the luminol-amplified chemiluminescence remained unchanged or was only slightly decreased under the same experimental conditions. Sodium azide strongly inhibited both kinds of luminescence. Hydroxyl radicals, formed in a Fenton reaction, may be important intermediates in the Type II native chemiluminescence.

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“…The vials were transiently opened to ambient air while the 0.5 ml aliquot of extract was quickly added, and the vial containing a total of 10 ml DMSO/TBAP, were then immediately closed, after which the initial CL of peak intensities were monitored at 22°C. This dilution operation in scintillation vials was reproducible and required about 2 s. Maximum wavelengths of emission were determined by interposition of plastic filters (Deneke and Krinsky, 1977;Kasha, 1948) of known transmittance ( Fig. 2C), followed by monitoring of CL.…”

Section: Measurement Of Chemiluminescencementioning

confidence: 99%

Extraction and analysis of superoxide free radicals (·O⁻₂) from whole mammalian liver

Shoaf

Shaikh

Harbison

et al. 1991

J. Biolumin. Chemilumin.

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Extraction of whole lobes of normal rat liver with dimethyl sulphoxide (DMSO) under N2 gives extracts which contain 5-10 mumol/l.O2- (50-100 nmol.O2- per 10 ml extract per 4 g liver; 1.25-2.50 nmol.O2- per millilitre per gram liver). Evidence for .O2- in the extracts is given by: (1) electron spin resonance signals (ESR), (2) differential pulse polarography (DPP), (3) chemiluminescence (CL), and (4) nitroblue tetrazolium reduction (NBT). All tests yield results identical with those obtained with authentic .O2-. Extraction of .O2- is enhanced by tetrabutyl ammonium ion, and is maximal at 1-3 min. These results raise the possibility that substantial amounts of .O2- are normally sequestered in protective membranous sites in vivo.

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INHIBITION AND ENHANCEMENT OF SINGLET OXYGEN (‘A_g) DIMOL CHEMILUMINESCENCE

Cited by 72 publications

References 22 publications

Chemiluminescence in hypoxic brain--the first report. Correlation between energy metabolism and free radical reaction.

Chemiluminescence in hypoxic brain--the first report. Correlation between energy metabolism and free radical reaction.

Increased native chemiluminescence in granulocytes isolated from synovial fluid and peripheral blood of patients with rheumatoid arthritis

Extraction and analysis of superoxide free radicals (·O⁻₂) from whole mammalian liver

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INHIBITION AND ENHANCEMENT OF SINGLET OXYGEN (‘Ag) DIMOL CHEMILUMINESCENCE

Cited by 72 publications

References 22 publications

Chemiluminescence in hypoxic brain--the first report. Correlation between energy metabolism and free radical reaction.

Chemiluminescence in hypoxic brain--the first report. Correlation between energy metabolism and free radical reaction.

Increased native chemiluminescence in granulocytes isolated from synovial fluid and peripheral blood of patients with rheumatoid arthritis

Extraction and analysis of superoxide free radicals (·O−2) from whole mammalian liver

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Product

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INHIBITION AND ENHANCEMENT OF SINGLET OXYGEN (‘A_g) DIMOL CHEMILUMINESCENCE

Extraction and analysis of superoxide free radicals (·O⁻₂) from whole mammalian liver