Inhibition-Based Biosensors for the Detection of Environmental Contaminants: Determination of 2,4-Dichlorophenoxyacetic Acid

Botrê, Claudio; Botrè, Francesco; Mazzei, Franco; Podestà, Elisabetta

doi:10.1897/1551-5028(2000)019<2876:ibbftd>2.0.co;2

Cited by 10 publications

(12 citation statements)

References 21 publications

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“…doi:10.1016/j.jelechem.2004.08.004 [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23], would be very welcome, since they could represent one of the most practical and inexpensive analytical devices, especially for the preliminary screening of huge numbers of samples.…”

Section: Introductionmentioning

confidence: 99%

“…We have previously published several inhibition based biosensors for the determination of different environmental pollutants, including marine toxins and pesticides [17][18][19][20][21][22][23] by enzyme inhibition studies; we are now proposing a further development of our approach, presenting two biodevices for the determination of pesticides based on the electrochemical monitoring of the catalytic activity and inhibition of alkaline phosphatase (ALP) by direct amperometric determination of the enzyme catalyzed reaction products.…”

Section: Introductionmentioning

confidence: 99%

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Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides

Mazzei

Botrè

Montilla

et al. 2004

Journal of Electroanalytical Chemistry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides

Mazzei

Botrè

Montilla

et al. 2004

Journal of Electroanalytical Chemistry

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“…Gulla et al studied the reactivation of inhibited enzymes for reusability of the biosensor [72]. Other pesticides of interest for biosensor development included 2,4 D [73].…”

Section: Enzyme-based Biosensorsmentioning

confidence: 99%

“…Glycoalkaloids were detected using a field-effect transistor as the transducer and butyrylcholinesterase as the biorecognition element [95]. Finally, phytotoxins, especially those produced by algae and found in seafood were of interest [96,97].…”

Section: Enzyme-based Biosensorsmentioning

confidence: 99%

Biosensors for environmental pollutants and food contaminants

Baeumner

2003

Analytical and Bioanalytical Chemistry

216

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This review article provides an overview of the most recent literature on biosensors for environmental pollutants and food contaminants. Due to the large number of publications, only papers published between 2000 and January 2003 were considered. Also, while not all of the published literature could be reviewed here, over 200 references are cited to provide a good overview of research undertaken in the last two years. Older publications are covered by a number of earlier review articles. This article provides an introduction into the field including specific consideration of the application areas, describes the typical biosensor assay format used, and is subsequently structured according to the biorecognition elements used (i.e., nucleic acids, enzymes, whole cells, tissue and whole organisms, antibodies and receptors, and biomimetic materials). In addition, a section on microbiosensing systems is provided. Since only very few microbiosensors with applications in environmental and food systems have been published, enabling technology is also covered in this article.

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“…2,4-dichlorophenoxyacetic acid (2,4-D) is one of the most widely applied phenoxy herbicides in many parts of the world (Wilson et al 1997b;Botrè et al 2000). In New Zealand, for instance, 68% of all pesticides used belong to the phenoxy herbicide class (Holland and Anis 1999).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of 2,4-dicholophenoxyacetic acid using an acidogenic anaerobic sequencing batch reactor

Chin¹,

Elefsiniotis²,

Singhal³

2005

Journal of Environmental Engineering and Science

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A bench-scale study was carried out to investigate the potential to biologically treat 2,4-dicholophenoxyacetic acid (2,4-D) contaminated wastewater in an anaerobic sequencing batch reactor (ASBR), operated in the acid-phase digestion mode. The effects of 2,4-D feed concentration (20 to 200 mg L −1 ) and temperature (ambient and 33 • C) on biodegradation were investigated at a hydraulic retention time of 48 h and a solids retention time of 10 d, using glucose as a supplemental substrate. Following a long acclimation period of about 100 d, complete 2,4-D degradation was observed at feed concentrations of 20 and 100 mg L −1 . However, at a 2,4-D concentration of 200 mg L −1 , only 65% removal was achieved. Overall, operation at an ambient temperature resulted in a slightly better performance than that at 33 • C. An adaptation period of approximately a week was required any time the 2,4-D concentration was increased, indicating a sensitive behavior towards shock loadings. On the other hand, glucose was completely and readily degraded throughout the study. A sequential utilization pattern of glucose and 2,4-D was also observed, with degradation of both substrates following first-order kinetics. Moreover, volatile fatty acids (VFAs) were the main products of acidogenesis, accounting for 65% of the effluent soluble chemical oxygen demand (COD), with acetic acid being by far the most predominant VFA detected.Résumé : Une étude en laboratoire a été effectuée afin d'examiner le potentiel de traitement biologique de l'acide (2,4-dichlorophénoxy)acétique (2,4-D) contaminant les eaux usées contenues dans un réacteur à séquençage discontinu anaérobie (ASBR) fonctionnant en mode de digestion en phase acide. Les effets de la concentration de l'alimentation en 2,4-D (de 20 à 200 mg L −1 ) et de la température (ambiante et 33 • C) sur la biodégradation ont été étudiés pour un temps de rétention hydraulique de 48 heures et un temps de rétention des matières solides de 10 jours, en utilisant le glucose comme substrat supplémentaire. Après une longue période d'acclimatation d'environ 100 jours, la dégradation totale du 2,4-D a été atteinte à des concentrations d'alimentation de 20 et de 100 mg L −1 . Cependant, à une concentration de 2,4-D de 200 mg L −1 , la dégradation n'atteignait que 65 %. Règle générale, l'opération à température ambiante a engendré un rendement légèrement meilleur qu'à 33˚C. Une période d'adaptation d'environ une semaine était requise à chaque fois que la concentration en 2,4-D était augmentée, indiquant ainsi une sensibilité aux surcharges. Mais le glucose a été complètement et rapidement dégradé durant toute l'étude. Un patron d'utilisation séquentiel du glucose et du 2,4-D a été observé, la dégradation de ces deux substrats suivant une cinétique du premier ordre. De plus, les acides gras volatils (ACV) constituaient les principaux produits de l'acidogénèse comptant pour 65 % de la demande chimique en oxygène (DCO) soluble de l'effluent, l'acide acétique étant de loin l'ACV le plus prédominant.

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Inhibition-Based Biosensors for the Detection of Environmental Contaminants: Determination of 2,4-Dichlorophenoxyacetic Acid

Cited by 10 publications

References 21 publications

Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides

Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides

Biosensors for environmental pollutants and food contaminants

Biodegradation of 2,4-dicholophenoxyacetic acid using an acidogenic anaerobic sequencing batch reactor

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