Inhibition by kasugamycin of initiation complex formation on 30S ribosomes

Okuyama, Atsushi; Machiyama, Nobuyoshi; Kinoshita, Tadatoshi; Tanaka, Nobuo

doi:10.1016/s0006-291x(71)80106-7

Cited by 109 publications

(48 citation statements)

References 3 publications

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“…Kasugamycin (KAS) inhibits initiation of polypeptide synthesis (32). Resistance mutations map to several loci in E. coli Overnight cultures of each strain were diluted into fresh M9 glucose medium and incubated at 37°C.…”

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confidence: 99%

Interfering with Different Steps of Protein SynthesisExplored by Transcriptional Profiling of Escherichia coli K-12

Sabina¹,

Dover

Templeton

et al. 2003

J Bacteriol

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Escherichia coli responses to four inhibitors that interfere with translation were monitored at the transcriptional level. A DNA microarray method provided a comprehensive view of changes in mRNA levels after exposure to these agents. Real-time reverse transcriptase PCR analysis served to verify observations made with microarrays, and a chromosomal grpE::lux operon fusion was employed to specifically monitor the heat shock response. 4-Azaleucine, a competitive inhibitor of leucyl-tRNA synthetase, surprisingly triggered the heat shock response. Administration of mupirocin, an inhibitor of isoleucyl-tRNA synthetase activity, resulted in changes reminiscent of the stringent response. Treatment with kasugamycin and puromycin (targeting ribosomal subunit association as well as its peptidyl-transferase activity) caused accumulation of mRNAs from ribosomal protein operons. Abundant biosynthetic transcripts were often significantly diminished after treatment with any of these agents. Exposure of a relA strain to mupirocin resulted in accumulation of ribosomal protein operon transcripts. However, the relA strain's response to the other inhibitors was quite similar to that of the wild-type strain.

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confidence: 99%

Interfering with Different Steps of Protein SynthesisExplored by Transcriptional Profiling of Escherichia coli K-12

Sabina¹,

Dover

Templeton

et al. 2003

J Bacteriol

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“…Loss of S2 increases the affinity of ribosomes for the cI transcript and decreases ribosome binding to leadered mRNA. Interestingly, alterations in S2 confer resistance to the antibiotic kasugamycin (16,27), an inhibitor of translation initiation (15,23). Furthermore, photoaffinity labeling studies show that S2 associates with the antibiotic pactamycin (24), which also inhibits translation initiation (2,23).…”

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confidence: 99%

Resistance of lambda cI translation to antibiotics that inhibit translation initiation

1993

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“…In the case of streptomycin a moderate inhibition of translocation was described by CABANAS et al149. On the other hand the aminoglycoside antibiotics, kasugamycin and spectinomycin which do not induce misread ing22,23) fail to inhibit translocation12, 24,25) In addition to the action of ST-F on translocation, aa-tRNA binding and induction of misreading it is still possible that ST-F might interfere with other steps of polypeptide synthesis.…”

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Action of streptothricin F on ribosomal functions.

Haupt¹,

Jonák

Rychlík

et al. 1980

J. Antibiot.

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The effect of streptothricin F on elongation factor-dependent and on elongation factor-free translation systems was studied. Streptothricin F inhibits factor-dependent as well as factorfree polypeptide synthesis. The results suggest that streptothricin F inhibits polypeptide synthesis via interaction with the ribosome. In partial reactions streptothricin F impairs EF-G-dependent translocation and to a lesser extent EF-T.dependent binding of as-RNA to the ribosome, while it does not affect peptide bond formation significantly.We recently examined the effect of streptothricin F (ST-F) on cell-free protein synthesis1). ST-F has been shown to inhibit protein synthesis specifically in intact bacterial cells and in cell-free systems of Escherichia coli, while the antibiotic did not inhibit cell-free protein synthesis in rat liver extracts.Furthermore ST-F induced misreading of synthetic homopolynucleot ides in E. co/i cell-free systems.In the present paper the action of ST-F on the individual reactions of polyphenylalanine formation on E. coli ribosomes is described. Materials and Methods Materials[14C] Phe-tRNA(182 mCi/m Mol) and ac["C]Phe-tRNA (182 mCi/m Mol) were prepared as described by CERNA et a1.2). Ac[14C]Leu-pentanucleotide (CACCA-acLeu, 164 mCi/m Mol) was prepared as described by MONRO et al.'). Radioactivity was determined in a Packard-Tricarb scintillation spectrometer (counting efficiency for 14C was 53 %) and a methane flow counter (Frieseke-Hoepfner, counting efficiency for 14C was 41 %).The antibiotics ST-F, neomycin, turimycin-H, and streptomycin were dissolved in water while oxytetracycline was dissolved in 0.01 N HCI. Antibiotic solutions were prepared immediately before use.Preparation of ribosomes Ribosomes were prepared from E. co/i B by ammonium sulfate precipitation according to GAVRI-LOVA and SPIRIN°) and GAVRILOVA et al.e1. The bacteria were broken by grinding with aluminium oxide powder and the ribosomes were pelleted by centrifugation at 105,000 g in a buffer containing 10 mM Tris-HCl (pH 7.8), 20 mm MgCl2, 5 mm NH4Cl and 1 mm B-mercaptoethanol.The ribosomal pellets were washed 4 times with 1 M NH,Cl in 10 mm Tris-HCl (pH 7.8), 10 mM MgCl2 and 1 mat mercaptoethanol and were precipitated at 0°-4°C by ammonium sulfate adding 49 g of the dry salt per 100 nil of the ribosome suspension containing 2.5 mg ribosomes per ml. The ribosomal precipitate was stored under ammonium sulfate at 4°C. Immediately before use 2 nil of the ribosome suspension were centrifuged for 40 minutes at 15,000 rpm. The pellet was suspended in 10 niM Tris-HCI (pH 7.8), 100 mm KCl and 20 mm MgCl2 and dialysed against the same buffer to complete removal of the ammonium sulfate. Dialysis was continued against a buffer with the MgCl2 concentration required for the experiment.

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Inhibition by kasugamycin of initiation complex formation on 30S ribosomes

Cited by 109 publications

References 3 publications

Interfering with Different Steps of Protein SynthesisExplored by Transcriptional Profiling of Escherichia coli K-12

Interfering with Different Steps of Protein SynthesisExplored by Transcriptional Profiling of Escherichia coli K-12

Resistance of lambda cI translation to antibiotics that inhibit translation initiation

Action of streptothricin F on ribosomal functions.

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