Inhibition of a novel model of murine experimental autoimmune orchitis by intravenous administration with a soluble testicular antigen: Participation of CD8+ regulatory T cells

Mukasa, Akiko; Itoh, Masahiro; Tokunaga, Yoshimasa; Hiramine, Chiharu; Hojo, Kenji

doi:10.1016/0090-1229(92)90074-x

Cited by 19 publications

(14 citation statements)

References 18 publications

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“…As the T/B ratio and %CD4 + cells were not changed by stimulation in vitro, the increase of CD4/CD8 ratio appeared to depend on the loss of CD8 + cells or the increase of CD4 − CD8 - T cells. Although the exact reason for these phenotypical changes remained unclear, the loss of CD8 + T cells, which are known to work as suppressor cells in certain circumstances,24 25 may break suppression of the development of EC. As expected, stimulation upregulated the expression of CD25, I-A, ICAM-1 (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Analysis of effects of stimulation in vitro of ovalbumin primed lymph node cells on adoptive transfer of experimental immune mediated blepharoconjunctivitis in Lewis rats

Yoshida¹,

Yoshida²,

Iwamoto³

et al. 1998

British Journal of Ophthalmology

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Aim-To analyse the role of stimulation in vitro of lymphocytes on the augmentation of experimental immune mediated blepharoconjunctivitis (EC, formerly EAC) in Lewis rats induced by adoptive transfer. Methods-Two weeks after immunisation with ovalbumin (OVA), rat draining lymph nodes were collected and 50 × 10 6 cells were injected into naive syngeneic recipients either directly or after culture in vitro with OVA, concanavalin A (Con A), or purified protein derivative (PPD) for 3 days. Four days after injection the rats were topically challenged with OVA. 24 hours later, they were sacrificed and eyes and spleens were harvested for histology and proliferation assay. In some experiments, naive recipient rats were irradiated with 7 Gy ray before transfer. The expression of adhesion molecules and cytokine profile of OVA primed lymph node cells were also investigated. Results-Both

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Section: Discussionmentioning

confidence: 99%

Analysis of effects of stimulation in vitro of ovalbumin primed lymph node cells on adoptive transfer of experimental immune mediated blepharoconjunctivitis in Lewis rats

Yoshida¹,

Yoshida²,

Iwamoto³

et al. 1998

British Journal of Ophthalmology

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“…injection of an antigen before immunization has been shown to represent a potent means to induce Ag-specific peripheral tolerance [3,4,14]. Many studies have reported its potential applicability to the treatment of autoimmune diseases [3,4,[14][15][16][17][18]. Investigation of the cellular mechanisms involved suggests that clonal anergy [5], together with thymic and peripheral T-cell apoptosis [19], are associated with systemic injection of highdose antigen.…”

Section: Discussionmentioning

confidence: 99%

“…After 24 h of culture, total RNA was recovered from cells using a single-step guanidine isothiocyanate-phenolchloroform isolation method developed by Chomczynski & Sacchi [13]. Reverse transcription of 1 g RNA was primed using oligo-(dT) [12][13][14][15][16][17][18] and different aliquots of cDNA were then used for PCR. The temperature profile of the amplification consisted of 25-30 cycles of: 1 min denaturation at 95ЊC, 30 s annealing at 56ЊC (␤-actin, IL-2), 58.5ЊC (IL-4) or 61ЊC (IFN-␥) and 1 min extension at 72ЊC.…”

Section: Methodsmentioning

confidence: 99%

Intravenous or Intranasal Administration of Gliadin is Able to Down‐Regulate the Specific Immune Response in Mice

Rossi

Maurano

Caputo³

et al. 1999

Scand J Immunol

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The mucosal lesion in coeliac disease (CD) represents an immunologically mediated injury triggered by gliadin and is restricted by a particular assortment of major histocompatibility complex (MHC) class II genes. Therefore, immunomodulatory strategies to tolerize gliadin‐specific, class II‐restricted T‐cell responses could represent an alternative to current treatments of CD, which are based on a gluten‐free diet. In this study, BALB/c mice derived from a gluten‐free diet colony were tolerized by either intranasal (i.n.) or intravenous (i.v.) administration of single or multiple doses of gliadin. While a single dose failed to induce tolerance, a significant decrease in gliadin‐specific T‐cell proliferation was detected (P < 0.001) after multiple i.n. or i.v. administrations. No significant difference in antibody titre was detected for antigen‐specific immunoglobulin G (IgG) or the IgG1 subclass, but a lower IgG2a‐specific titre was observed. Both interferon‐γ (IFN‐γ) and interleukin (IL)‐2 expression, measured by enzyme‐linked immunosorbent assay (ELISA) and reverse transcription–polymerase chain reaction (RT–PCR), were reduced on antigen administration, both i.v. and i.n. Neither regimen showed a regulatory effect on IL‐4 production. As T helper 1 (Th1) cytokines seem to be important in the pathogenesis of CD, our data therefore highlight the potential of i.n. and i.v. routes for the design of useful immunomodulatory strategies for CD.

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“…As antigen‐induced models of autoimmune disease were devised over the subsequent years, the effect of pre‐treatment with the same antigen was explored. Seemingly without exception, this strategy has resulted in protection from disease in a large range of animal models tested 3–7 …”

Section: Developing the Principle Of Antigen‐specific Immunotherapy Fmentioning

confidence: 99%

Antigen‐specific immunotherapy for autoimmune disease: fighting fire with fire?

Peakman

Dayan

2001

Immunology

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Inhibition of a novel model of murine experimental autoimmune orchitis by intravenous administration with a soluble testicular antigen: Participation of CD8+ regulatory T cells

Cited by 19 publications

References 18 publications

Analysis of effects of stimulation in vitro of ovalbumin primed lymph node cells on adoptive transfer of experimental immune mediated blepharoconjunctivitis in Lewis rats

Analysis of effects of stimulation in vitro of ovalbumin primed lymph node cells on adoptive transfer of experimental immune mediated blepharoconjunctivitis in Lewis rats

Intravenous or Intranasal Administration of Gliadin is Able to Down‐Regulate the Specific Immune Response in Mice

Antigen‐specific immunotherapy for autoimmune disease: fighting fire with fire?

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