Inhibition of Acetylcholinesterase and Butyrylcholinesterase by Chlorpyrifos-oxon

Amitai, Gabriel; Moorad, Deborah R.; Adani, R.; Doctor, Bhupendra P.

doi:10.1016/s0006-2952(98)00035-5

Cited by 151 publications

(88 citation statements)

References 23 publications

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“…Their values for fetal bovine serum, human erythrocyte, Torpedo californica, recombinant mouse, and recombinant human AChE were 2.2, 3.8, 8.0, 5.1, and 9.3 µM − 1 min − 1 , respectively. Adjusting the k i obtained in the present work to account for the temperature difference between the two studies (Aldridge & Reiner, 1972) yields a value of 7.4 µM −1 min −1 for hen brain microsomal AChE, which is within the range obtained by Amitai et al (1998). Capodicasa et al (1991) investigated the time course of inhibition of hen brain AChE and NTE by CPO under conditions that gave nonlinear kinetics and determined 20-min I 50 values of 6 and 150 nM for AChE and NTE, respectively.…”

Section: Notesupporting

confidence: 75%

“…With respect to measurements of the inhibitory potency of CPO, Amitai et al (1998) measured the k i of this compound against different sources of purified AChE at 25°C. Their values for fetal bovine serum, human erythrocyte, Torpedo californica, recombinant mouse, and recombinant human AChE were 2.2, 3.8, 8.0, 5.1, and 9.3 µM − 1 min − 1 , respectively.…”

Section: Notementioning

confidence: 99%

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Relative Inhibitory Potencies of Chlorpyrifos Oxon, Chlorpyrifos Methyl Oxon, and Mipafox for Acetylcholinesterase Versus Neuropathy Target Esterase

Kropp

Richardson

2003

Journal of Toxicology and Environmental Health, Part A

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Section: Notesupporting

confidence: 75%

Section: Notementioning

confidence: 99%

Relative Inhibitory Potencies of Chlorpyrifos Oxon, Chlorpyrifos Methyl Oxon, and Mipafox for Acetylcholinesterase Versus Neuropathy Target Esterase

Kropp

Richardson

2003

Journal of Toxicology and Environmental Health, Part A

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“…It is nonsystemic broad spectrum, general use pesticide that disrupts the nervous system function by inhibiting cholinesterase enzyme that normally terminates nerve transmission by cleaving the neurotransmitter acetylcholine and resultant cholinesterase accumulation [4,5]. It is an active ingredient in the Dursban, Lorsban, and Predator brand of pesticides [6].…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Effects of Chlorpyrifos in Freshwater Fish Cirrhinus mrigala Using Micronucleus Assay

Bhatnagar

Yadav

Cheema

2016

Advances in Biology

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The genotoxicity of pesticides is an issue of worldwide concern and chlorpyrifos is one of the largest selling organophosphate agrochemicals that has been widely detected in surface waters of India. The studies on long term genotoxic biomarkers are limited; therefore, present study was carried out to analyze the incidence of nuclear anomalies in the blood cells of fresh water fish Cirrhinus mrigala using micronucleus (MN) assay as a potential tool for assessment of genotoxicity. Acute toxicity of chlorpyrifos was evaluated by exposing fingerlings to different doses of chlorpyrifos (1/20, 1/10, and 1/5 of LC 50 ) and LC 50 was calculated as 0.44 mg L −1 using probit analysis. Blood samples were taken on days 2, 4, 8, 12, 21, 28, and 35. In general, significant effects for both concentration and duration of exposure were observed in treated fish. It was found that MN induction was highest on day 14 at 0.08 mg L −1 concentration of chlorpyrifos. It was concluded that chlorpyrifos is genotoxic pesticide causing nuclear anomalies in Cirrhinus mrigala.

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“…BuChE was expressed in CHO K1 cells and purified from serum-free medium by affinity chromatography and ion exchange chromatography on DE52 (XIE et al, 1999). Enzyme active site concentration [E] of highly purified preparation was titrated at residual activity after inhibition by chlorpyrifosoxon (AMITAI et al, 1998).…”

Section: Enzymesmentioning

confidence: 99%

Inter-tissue and inter-species comparison of butyrylcholinesterases

et al. 2006

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Inhibition of Acetylcholinesterase and Butyrylcholinesterase by Chlorpyrifos-oxon

Cited by 151 publications

References 23 publications

Relative Inhibitory Potencies of Chlorpyrifos Oxon, Chlorpyrifos Methyl Oxon, and Mipafox for Acetylcholinesterase Versus Neuropathy Target Esterase

Relative Inhibitory Potencies of Chlorpyrifos Oxon, Chlorpyrifos Methyl Oxon, and Mipafox for Acetylcholinesterase Versus Neuropathy Target Esterase

Genotoxic Effects of Chlorpyrifos in Freshwater Fish Cirrhinus mrigala Using Micronucleus Assay

Inter-tissue and inter-species comparison of butyrylcholinesterases

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