Inhibition of acrolein-stimulated MUC5AC expression by Platycodon grandiflorum root-derived saponin in A549 cells

Choi, Jae Ho; Hwang, Yong Pil; Han, Eun Hee; Kim, Hyung Gyun; Park, Bong Hwan; Lee, Hyun Sun; Park, Byung Keun; Lee, Young Chun; Chung, Young Chul; Jeong, Hye Gwang

doi:10.1016/j.fct.2011.05.030

Cited by 34 publications

(23 citation statements)

References 52 publications

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“…The event was further evaluated by specific inhibition of JNK expression by an in vitro siRNA JNK kinase. Although MUC5AC activation by MAP Kinase has been established by previous studies in other cells lines, 25,26,30) it has remained unclear in BEAS-2B cells. Interestingly, Choi et al demonstrated that p38 kinase has a key role on the negative regulation of MUC5AC gene expression in A549 cells.…”

Section: )mentioning

confidence: 99%

“…Interestingly, Choi et al demonstrated that p38 kinase has a key role on the negative regulation of MUC5AC gene expression in A549 cells. 30) Therefore, we suggest that the regulation of MUC5AC has distinct pathway depending on the type of stresses and cell types. The defined molecular mechanism needs to be investigated in-depth.…”

Section: )mentioning

confidence: 99%

“…It is known that various transcriptional factors such as NF-κB, AP-1, and SP1 are responsible for regulation of MUC5AC gene expression in different airway epithelial cells in response to verities of stimuli. 25,26,30) In particular NF-κB sites on MUC promoter site perform a crucial role in regulation of MUC expression in human bronchial epithelial cells. 31) In this study, PQ can induce phosphorylation of NF-κB and its nuclear translocation along with MUC5AC gene expression.…”

Section: )mentioning

confidence: 99%

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Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells via Modulating Expression of MUC5AC

Podder

Song

et al. 2014

Biological & Pharmaceutical Bulletin

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Mucins are highly glycosylated secretary proteins produced by most epithelial cells. Hypersecretion of mucins is one of the prominent symptoms of several airway diseases, including asthma, cystic fibrosis, nasal allergy, rhinitis, and sinusitis. Paraquat (PQ), a common herbicide, has been associated with pulmonary damage and is a potent reactive oxygen species (ROS) producer. However, until now the role of PQ on mucin overproduction has not been studied. The aim of this study is to explore how kaempferol (KM), a widely used dietary flavonoid, affects the protection of human PQ-exposed bronchial epithelium BEAS-2B cells by suppressing Mucin gene expression via nuclear factor-kappa B (NF-κB). We observed that PQ generates intracellular ROS, and also induces lipid peroxidation in BEAS-2B cells. Additionally, we found that PQ effectively induces the expression of the MUC5AC gene; however, co-treatment of PQ with KM drastically reduces its expression. Furthermore, we observed that PQ activates NF-κB, while co-treatment with KM occludes its nuclear translocation, and additionally KM repressed the PQ phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) in BEAS-2B cells. Based on our data, we believe that KM can suppress the over-expression of the MUC5AC gene. This would contribute to the protection of PQ cytotoxicity to exposed BEAS-2B cells, and allow further study toward a better understanding of ROSassociated diseases.Key words MUC5AC; kaempferol; reactive oxygen species; human bronchial epithelial BEAS-2B cell; nuclear factor-kappa B Several studies have been carried out demonstrating hypersecretion of mucous from respiratory epithelial cells a major pathological event in chronic airway diseases such as chronic obstructive pulmonary diseases or asthma.1,2) These pathological manifestations are governed by the interaction between underlying genetic factors and xenobiotics, which together are responsible for intercellular reactive oxygen species (ROS) production.3) Findings have shown that ROS such as: hydrogen peroxide, superoxide anion, hydroxyl ion are responsible for the initiation and progression of chronic airway diseases via over-expression of Mucin genes.4-6) ROS is a major etiological factor in the pathogenesis of chronic inflammatory airway diseases as it triggers mucin secretion in epithelia. 7)Twenty different MUC genes have been characterized in human airway epithelia, these are then subdivided into membrane-bound and secreted forms.8-11) MUC5AC is a hallmark gene, and considered the most phenotypical gene in respiratory diseases; it accumulates in respiratory tract can lead to symptoms such as chronic cough and excessive sputum production. Previous studies showed that paraquat (PQ) is a potent ROS inducer in different epithelial cell lines [12][13][14] and is associated with cellular cytoxicity. However, there has been no study into whether PQ can induce MUC gene expression or not, so in this study we investigated the effects of PQ on Bronchial epithelial BEAS...

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Section: )mentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Section: )mentioning

confidence: 99%

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Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells via Modulating Expression of MUC5AC

Podder

Song

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Rottlerin is also very potent in blocking other kinases including Akt/PKB and p38 MAPK [29–31]. It also inhibits human T cell responses [32], reduces MUC5AC expression in human epithelial cells [33], abrogates reactive oxygen species production in hepatic stellate cells [34], and prevents histamine-induced H1-receptor gene expression in HeLa cells [35]. However, still very limited information is available of rottlerin towards cancer disease and its mechanism of action.…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Mallotus philippinensisMuell. Arg (Euphorbiaceae): Ethnopharmacology and Phytochemistry Review

Gangwar

Goel

Nath

2014

BioMed Research International

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Mallotus philippinensis Muell. Arg (Euphorbiaceae) are widely distributed perennial shrub or small tree in tropical and subtropical region in outer Himalayas regions with an altitude below 1,000 m and are reported to have wide range of pharmacological activities. Mallotus philippinensis species are known to contain different natural compounds, mainly phenols, diterpenoids, steroids, flavonoids, cardenolides, triterpenoids, coumarins, isocoumarins, and many more especially phenols; that is, bergenin, mallotophilippinens, rottlerin, and isorottlerin have been isolated, identified, and reported interesting biological activities such as antimicrobial, antioxidant, antiviral, cytotoxicity, antioxidant, anti-inflammatory, immunoregulatory activity protein inhibition against cancer cell. We have selected all the pharmacological aspects and toxicological and all its biological related studies. The present review reveals that Mallotus philippinensis is a valuable source of medicinally important natural molecules and provides convincing support for its future use in modern medicine. However, the existing knowledge is very limited about Mallotus philippinensis and its different parts like steam, leaf, and fruit. Further, more detailed safety data pertaining to the acute and subacute toxicity and cardio- and immunotoxicity also needs to be generated for crude extracts or its pure isolated compounds. This review underlines the interest to continue the study of this genus of the Euphorbiaceae.

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“…Anti-inflammatory, antiviral, antiapoptotic, and/or antioxidant activities could also be found in minor components like Nepeta cataria L. [34], Pericarpium Citri Reticulatae [35, 36], Radix Glycyrrhizae Preparata [37], Tussilago farfara L. [38], Folium Perillae [39], and Zingiber officinale Roscoe [40]. Furthermore, saponin derived from root of Platycodon grandiflorus and alkaloids extracted from Fritillaria plants inhibit Muc5AC expression [41]. However, more works are needed to identify the components regulating AQPs expression, because this effect has not been attributed to any of the herbs or herbs extracts involved in this formula.…”

Section: Discussionmentioning

confidence: 99%

Effects of Modified Zhisou Powder on Airway Mucus Production in Chronic Obstructive Pulmonary Disease Model Rats with Cold‐Dryness Syndrome

Gao

Wang

Jing

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Objective. In China, the Chinese medicine formula modified zhisou powder (MZP) is commonly used to treat COPD with cold-dryness syndrome (CDSCOPD) to relieve cough and sputum. However, the underlying mechanisms of MZP on treating CDSCOPD remain to be elucidated. Methods. COPD and CDSCOPD rat models were established; MZP was given to CDSCOPD rats in the last 7 days of the 97-day model establishment. Then the rats were subjected to lung function measurement. Pathological changes in lungs were observed through paraffin section and H&E staining. The mRNA and protein levels of AQP1, 4, and 5 and Muc5AC and Muc5B in lung were determined by quantitative RT-PCR and western blotting. NE levels was determined by ELISA. Results. The impaired lung functions were observed in rats exposed to cigarette smoke. Among all parameters evaluating lung functions, only tidal volume demonstrates a further decrease in CDSCOPD when compared with COPD, indicating further impaired pulmonary ventilation functions upon cold-dryness stimulation. The intervention of MZP effectively improved lung functions parameters, prevented the inflammations, and eliminated the increases of AQP4 and 5 and the decrease of Muc5AC in lung. Conclusion. MZP probably improves pulmonary functions in CDSCOPD through inhibiting lung inflammation, increasing expressions of AQPs, and decreasing Muc5AC expression in lung.

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Inhibition of acrolein-stimulated MUC5AC expression by Platycodon grandiflorum root-derived saponin in A549 cells

Cited by 34 publications

References 52 publications

Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells <i>via</i> Modulating Expression of <i>MUC5AC</i>

Cytoprotective Effect of Kaempferol on Paraquat-Exposed BEAS-2B Cells <i>via</i> Modulating Expression of <i>MUC5AC</i>

Mallotus philippinensisMuell. Arg (Euphorbiaceae): Ethnopharmacology and Phytochemistry Review

Effects of Modified Zhisou Powder on Airway Mucus Production in Chronic Obstructive Pulmonary Disease Model Rats with Cold‐Dryness Syndrome

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