Inhibition of amyloid β aggregation and protective effect on SH-SY5Y cells by triterpenoid saponins from the cactus Polaskia chichipe

Fujihara, Koji; Koike, Sachiko; Ogasawara, Yuki; Takahashi, Kunio; Koyama, Kiyotaka; Kinoshita, Kaoru

doi:10.1016/j.bmc.2017.04.023

Cited by 18 publications

(17 citation statements)

References 45 publications

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“…Compounds that are able to reduce excessive levels of ROS, Aβ production, oligomerization, neurotoxicity and inflammation induced by Aβ and inhibit tau protein hyperphosphorylation are potential targets for the discovery of new drugs with anti-AD properties [1,2,6,12,18]. A number of medicinal plants in the form of extracts, fractions, or isolated compounds have been reported as presenting possible anti-AD activity in preclinical and clinical studies [5].…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

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Alzheimer’s disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia , Limonium brasiliense , Paullinia cupana , Poincianella pluviosa , Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ 25–35 toxicity in SH-SY5Y cells. The effect of the EAF of S . adstringens on mitochondrial membrane potential, lipid peroxidation, superoxide production and mRNA expression of 10 genes related to AD was also evaluated and the electropherogram fingerprints of EAFs were established by capillary electrophoresis. Chemometric tools were used to correlate the in vitro activities of the samples with their potential to be evaluated against AD and to divide extracts/fractions into four clusters. Pretreatment with the EAFs grouped in cluster 1 ( S . adstringens , P . pluviosa and L . brasiliense ) protected SH-SY5Y cells from Aβ 25-35 -induced toxicity. The EAF of S . adstringens at 15.62 μg/mL was able completely to inhibit the mitochondrial depolarization (69%), superoxide production (49%) and Aβ 25-35 -induced lipid peroxidation (35%). With respect to mRNA expression, the EAF of S . adstringens also prevented the MAPT mRNA overexpression (expression ratio of 2.387x) induced by Aβ 25–35 , which may be related to tau protein hyperphosphorylation. This is the first time that the neuroprotective effects of these fractions have been demonstrated and that the electropherogram fingerprints for the EAFs of G . ulmifolia , L . brasiliense , P . cupana , P . pluviosa and S . adstringens have been established. The study expands knowledge of the in vitro protective effects and quality control of the evaluated fractions.

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Section: Introductionmentioning

confidence: 99%

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

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“…Ursolic acid is a triterpenoid associated with antiproliferative, anti-inflammatory, and apoptosis-inducing activities, as well as with management of neurodegenerative diseases [36,37]. Further, saponins have been associated with a protective effect against oxidative stress in neurons, exhibiting neuroprotective action by inhibiting Aβ-amyloid and by suppressing lipid peroxide accumulation and GSH reduction on mice hippocampus induced by Aβ 25–35 protein [7,38].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antioxidant, Anti-Biofilm, and Solar Protection Activities of Melocactus zehntneri (Britton & Rose) Pulp Extract

et al. 2019

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Cactaceae plants are important due to their nutritional and therapeutic values. This study aimed to identify the phytochemical profile and biological activities of six Melocactus zehntneri pulp extracts: hexane extract (HE), chloroform extract (CE), ethanol extract (EE), methanol extract (ME), final water extract (FWE), and water extract (WE). Sugar, phenolic compounds, and protein content of the extracts were determined. Then thin layer chromatography (TLC) was performed to detect the presence of terpenes (ursolic and oleanolic acids), saponins, sugars, and glycoproteins. These extracts were analyzed for antioxidant activity via in vitro assay. HE showed 75% ferric chelating activity. All extracts showed 80–100% superoxide and hydroxyl radical-scavenging activities, respectively. Further, all extracts at 25 µg/mL showed 60% activity against DPPH. Moreover, in the 3T3 cells lines, no cytotoxicity was observed; however, therapeutic activity against the effects of the H2O2 treatment was exhibited. Finally, the polar extracts (EE, ME, FWE, and WE), particularly WE, elicited activity against the biofilms of Staphylococcus epidermidis, and HE and CE expressed a capacity for solar protection.

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“…Considering those reports, we had been further investigating triterpene sapogenins from many cacti and discovered that S. pruinosus contained erythrodiol, longispinogenin and 3β-hydroxy-11α,12α-epoxyolean-28,13β-olide in addition to oleanolic acid from hydrolysate of MeOH extract of S. pruinosus [4]. Now, we have been investigating triterpene saponins from cacti for several decades [5][6][7][8][9][10][11][12] and reported the identification of numerous saponins exhibiting bioactivities such as anti-type I allergy activity [7], inhibition or promotion of melanin synthesis [5], inhibition of amyloid β (Aβ) aggregation, and protective effects on SH-SY5Y cells against Aβ-associated toxicity [6].…”

Section: Introductionmentioning

confidence: 99%

“…The activities of saponins have been well studied and include anti-inflammatory, anti-tumor, anti-obesity, anti-angiogenesis, anti-allergic, anti-microbial, and anti-Alzheimer's disease (AD) [13,14]. In particular, our group found that saponins from cacti have unique structures [5][6][7][8][9][10][11][12] that have not been adequately investigated.…”

Section: Introductionmentioning

confidence: 99%

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Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus

et al. 2020

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Six new triterpene saponins (1-5,7) and 3 known saponins (6,8,9) were isolated from MeOH extracts of the cactus Stenocereus pruinosus. The structures of the isolated saponins were elucidated using MS, IR, and comprehensive NMR measurements. To develop drugs for treating Alzheimer's disease (AD) on the basis of the amyloid cascade hypothesis, the isolated saponins were evaluated for inhibition of BACE1 activity and amyloid beta (Aβ) aggregation using thioflavin-T assay, and triterpenes as an aglycone moiety and an alkaline hydrolysate of the saponins were also evaluated. One saponin, stenoside A (7), exhibited inhibitory activity related to Aβ aggregation and its degree of Aβ aggregation was 40.6% at 100 μM.

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Inhibition of amyloid β aggregation and protective effect on SH-SY5Y cells by triterpenoid saponins from the cactus Polaskia chichipe

Cited by 18 publications

References 45 publications

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

In Vitro Antioxidant, Anti-Biofilm, and Solar Protection Activities of Melocactus zehntneri (Britton & Rose) Pulp Extract

Amyloid β aggregation inhibitory activity of triterpene saponins from the cactus Stenocereus pruinosus

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