Inhibition of aortic intimal hyperplasia and cell cycle protein and extracellular matrix protein expressions by BuYang HuanWu Decoction

Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression

“…As a classic TCM formulation of invigorating Qi and activating blood, BYHWD can be used to treat many disorders with Qi deficiency and blood stasis, which is likely to target the specific pathogenesis of vascular diseases (11). BYHWD was previously shown to inhibit aortic intimal hyperplasia and cellular proliferation in cultured VSMC (15,16).…”

“…(11), the underlying molecular mechanisms of which relies on its pharmacological role in anti-inflammation, antioxidative, anti-apoptosis and anti-angiotensin II (Ang II) (12)(13)(14). Of note, it has been shown that BYHWD could inhibit VSMC proliferation caused by injury-or plateletderived growth factor (15,16). In this scenario, there is potential role for BYHWD to control age-associated migration and invasion of serum containing BYHWD (VSMCs).…”

The effects of BuYang HuanWu Decoction and its effective components on proliferation-related factors and ERK1/2 signal transduction pathway in cultured vascular smooth muscle cells

“…Diels, Umbelliferae), 9 g Flos Carthami (Carthamus tincforius L., Compositae), 9 g Semen Persicae (Prunus persica (L.) Batsch, Rosaceae), and 9 g Lumbricus (Pheretima aspergillum (E.Perrier), Megascolecidae). The location, collection and voucher specimen of material of BYHWD were the same with our study before (Wu et al, 2009). The content of BYHWD effective components, alkaloids and glycosides, were determined by high performance liquid chromatography (HPLC) as described in our previous study (Wu et al, 2009).…”

“…The location, collection and voucher specimen of material of BYHWD were the same with our study before (Wu et al, 2009). The content of BYHWD effective components, alkaloids and glycosides, were determined by high performance liquid chromatography (HPLC) as described in our previous study (Wu et al, 2009). The concentration of crude drug in the extracting solution was 1 g/mL.…”

“…Previous research has shown that alkaloids and glycosides are the major effective components of BYHWD, the alkaloids and glycosides extracted from BYHWD can inhibit the intima hyperplasia injured by balloon catheter, the proliferation of VSMC and the synthesis of ECM after intima injury (Wu et al, 2009). As VSMC has an important role in the occurrence of vascular proliferative diseases, this study used platelet derived growth factor (PDGF)-induced VSMC proliferation as a model in vitro to observe the impact of BYHWD and its effective components alkaloids and glycosides on cell cycle.…”