Inhibition of attachment of oral bacteria to immortalized human gingival fibroblasts (HGF-1) by tea extracts and tea components

Wang, Yi; Chung, Felicia Fei‐Lei; Lee, Sui Mae; Dykes, Gary A.

doi:10.1186/1756-0500-6-143

Cited by 20 publications

(10 citation statements)

References 24 publications

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“…Methyl gallate at concentrations of 1–4 mg/mL rendered biofilm formation of S. mutans to up to 80% [178]. Green and oolong tea contain substantial quantities of gallic acid and epigallocatechin gallate and have exhibited slight inhibition effect on the attachment of S. mutans and other oral bacterial to collagen, tooth surfaces and gingival cell line [180]. In the same study, fermented tea with high tannin content opposed to green tea and oolong tea had shown more activity towards attachment of S. mutans and other oral bacterial to collagen, tooth surfaces and gingival cell line [180].…”

Section: Anti-streptococcal Attributes Of Phytochemicalsmentioning

confidence: 99%

“…Successful inhibition of adhesion and hydrophobic interactions could reduce and or prevent sore throat caused by S. pyogenes [164]. It has been suggested that polymeric flavonoids or other large molecule polyphenols may exhibit higher anti-adhesion effects against streptococci [180]. Coffee high molecular weight fraction nearly completely (91%) hindered the adhesion of S. mutans [184].…”

Section: Anti-streptococcal Attributes Of Phytochemicalsmentioning

confidence: 99%

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Molecular Mechanisms of Inhibition of Streptococcus Species by Phytochemicals

2016

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This review paper summarizes the antibacterial effects of phytochemicals of various medicinal plants against pathogenic and cariogenic streptococcal species. The information suggests that these phytochemicals have potential as alternatives to the classical antibiotics currently used for the treatment of streptococcal infections. The phytochemicals demonstrate direct bactericidal or bacteriostatic effects, such as: (i) prevention of bacterial adherence to mucosal surfaces of the pharynx, skin, and teeth surface; (ii) inhibition of glycolytic enzymes and pH drop; (iii) reduction of biofilm and plaque formation; and (iv) cell surface hydrophobicity. Collectively, findings from numerous studies suggest that phytochemicals could be used as drugs for elimination of infections with minimal side effects.

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Section: Anti-streptococcal Attributes Of Phytochemicalsmentioning

confidence: 99%

Section: Anti-streptococcal Attributes Of Phytochemicalsmentioning

confidence: 99%

Molecular Mechanisms of Inhibition of Streptococcus Species by Phytochemicals

2016

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“…There are previous published studies on the activity of tea components against bacteria responsible for oral diseases. Some research groups showed that tea extracts inhibited the attachment of bacteria to oral surfaces [32] and are effective against cariogenic microorganisms [23]. It was observed that rinsing the mouth with green tea led to a significant reduction of cariogenic bacteria like S. mutans and Lactobacillus [19,33].…”

Section: Microbiological Assays – Determination Of Minimum Inhibitorymentioning

confidence: 99%

Inhibitory activity of a green and black tea blend on Streptococcus mutans

Barroso

Ramalhete

Domingues³

et al. 2018

Journal of Oral Microbiology

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Through the years, tea consumption has been associated with good health, and some publications are related to oral health. The bioactive components of green tea are thought to be able to influence the process of caries formation through inhibition of proliferation of the streptococcal agent, interference with the process of bacterial adhesion to tooth enamel, and inhibition of glucosyl transferase and amylase; however, little is known about black tea and oral health. The aim of the present in-vitro study was to determine the inhibitory activity of a novel, patent-pending and proprietary blend of green and black tea aqueous extracts on Streptococcus mutans, a bacterium widely associated with plaque development and tooth decay. A minimum inhibitory concentration (MIC) of 12.5 mg/mL and a minimum bactericidal concentration (MBC) of 12.5 mg/mL was established against S. mutans, meaning that at concentrations of 12.5 mg/mL and higher, the proprietary tea blend is effective against the growth of S. mutans. This MIC concentration is lower than the ones reported in the literature for alcoholic black tea and green tea extracts tested separately. As a promising natural ingredient for oral health, this finding is a good indicator for the use of this proprietary blend of black and green tea water extracts.

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“…In these methods, a slide of the substratum material is immersed in a bacterial suspension, with or without shaking, to allow attachment. Attached cells are enumerated by direct microscopic observation (Absolom et al, 1983;Clark & Gibbons, 1977;Hood & Zottola, 1997;Wang et al, 2013a) or by spread plating after detaching the cells from the surface by stomaching or sonication (Cassat et al, 2007;Chia et al, 2011;Faille et al, 2002;Morra et al, 1996;Wang et al, 2013b).…”

Section: Quantification Of Bacterial Attachmentmentioning

confidence: 99%

The physicochemical process of bacterial attachment to abiotic surfaces: Challenges for mechanistic studies, predictability and the development of control strategies

Wang¹,

Lee²,

Dykes³

2014

Critical Reviews in Microbiology

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Bacterial attachment to abiotic surfaces can be explained as a physicochemical process. Mechanisms of the process have been widely studied but are not yet well understood due to their complexity. Physicochemical processes can be influenced by various interactions and factors in attachment systems, including, but not limited to, hydrophobic interactions, electrostatic interactions and substratum surface roughness. Mechanistic models and control strategies for bacterial attachment to abiotic surfaces have been established based on the current understanding of the attachment process and the interactions involved. Due to a lack of process control and standardization in the methodologies used to study the mechanisms of bacterial attachment, however, various challenges are apparent in the development of models and control strategies. In this review, the physicochemical mechanisms, interactions and factors affecting the process of bacterial attachment to abiotic surfaces are described. Mechanistic models established based on these parameters are discussed in terms of their limitations. Currently employed methods to study these parameters and bacterial attachment are critically compared. The roles of these parameters in the development of control strategies for bacterial attachment are reviewed, and the challenges that arise in developing mechanistic models and control strategies are assessed.

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Inhibition of attachment of oral bacteria to immortalized human gingival fibroblasts (HGF-1) by tea extracts and tea components

Cited by 20 publications

References 24 publications

Molecular Mechanisms of Inhibition of Streptococcus Species by Phytochemicals

Molecular Mechanisms of Inhibition of Streptococcus Species by Phytochemicals

Inhibitory activity of a green and black tea blend on Streptococcus mutans

The physicochemical process of bacterial attachment to abiotic surfaces: Challenges for mechanistic studies, predictability and the development of control strategies

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