Inhibition of Autophagy Promoted Apoptosis and Suppressed Growth of Hepatocellular Carcinoma Upon Photothermal Exposure

Wang, Yuqian; Zhao, Hong; Wang, Dezhou; Hao, Miao; Kong, Chenfei; Zhao, Xiaoming; Gao, Yiyao; Li, Jing; Liu, Baocai; Bai, Yifeng; Zhang, Hao; Jiang, Jinlan

doi:10.1166/jbn.2019.2714

Cited by 28 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…16 The expression of glucose transporter-1 (GLUT-1) is associated with autophagy activation in CSCs under hypoxic or nutrientdeprived conditions. [17][18][19][20] Autophagy may also affect cellular glucose uptake. [21][22][23] Beclin-1, an autophagy marker, plays an important role in the initiation of autophagy.…”

Section: Introductionmentioning

confidence: 99%

Effect of GLUT1 Inhibition and Autophagy Modulation on the Growth and Migration of Laryngeal Carcinoma Stem Cells Under Hypoxic and Low-Glucose Conditions

Chen

Liu

Zhong

et al. 2021

OTT

View full text Add to dashboard Cite

Background: Enhanced glucose uptake and autophagy are means by which cells adapt to stressful microenvironments. In this study, we investigated the roles of glucose transporter-1 (GLUT-1) and autophagy in laryngeal carcinoma stem cells under hypoxic and low-glucose conditions. Materials and Methods: CD133-positive Tu212 laryngeal carcinoma stem cells were purified by magnetic-activated cell sorting and subjected to hypoxic and/or low-glucose conditions. Proliferation was evaluated using a cell-counting kit and a clone-formation assay, and migration capability was measured through a Transwell assay. Autophagy was assessed using transmission electron microscopy. Gene silencing was monitored using shRNA technology and autophagy regulation was manipulated using rapamycin, 3-MA, or chloroquine. Gene expression levels were evaluated by quantitative reverse transcriptionpolymerase chain reaction and protein levels were assessed via Western blotting. Results: Compared to CD133-negative cells, CD133-positive cells showed increased proliferation and migration capabilities, and reduced apoptosis, under hypoxic or low-glucose conditions. CD133-positive cells also showed increased expression of GLUT-1 and autophagy activity. Finally, GLUT-1 knockdown or autophagy inhibition reduced the proliferation and migration of CD133-positive laryngeal carcinoma stem cells. Conclusion: Enhanced glucose uptake and autophagy maintain the tumor behaviors of CD133-positive laryngeal carcinoma stem cells under hypoxic and low-glucose conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of GLUT1 Inhibition and Autophagy Modulation on the Growth and Migration of Laryngeal Carcinoma Stem Cells Under Hypoxic and Low-Glucose Conditions

Chen

Liu

Zhong

et al. 2021

OTT

View full text Add to dashboard Cite

show abstract

“…Among these approaches, the combination of autophagy-modulating molecules and mild PTT shows the satisfactory ability of eliminating tumors at gentle temperatures [ 129 – 132 ]. Some studies have figured out that low-temperature PTT can induce both apoptosis and autophagy in cancer cells, and autophagy provides a survival way for malignant tumors [ 129 , 130 ]. Further, it has been revealed that the inhibition of autophagy can augment apoptosis and apparently strengthen the efficiency of PTT, verified by the lifted malignant cells' fatality under mild hyperthermia using the autophagy inhibitor chloroquine (CQ) [ 129 ] and chloroquine diphosphate-loaded NPs [ 130 ].…”

Section: Applications Of Low-temperature Pttmentioning

confidence: 99%

Low-Temperature Photothermal Therapy: Strategies and Applications

Duan

2021

Research

150

View full text Add to dashboard Cite

Although photothermal therapy (PTT) with the assistance of nanotechnology has been considered as an indispensable strategy in the biomedical field, it still encounters some severe problems that need to be solved. Excessive heat can induce treated cells to develop thermal resistance, and thus, the efficacy of PTT may be dramatically decreased. In the meantime, the uncontrollable diffusion of heat can pose a threat to the surrounding healthy tissues. Recently, low-temperature PTT (also known as mild PTT or mild-temperature PTT) has demonstrated its remarkable capacity of conquering these obstacles and has shown excellent performance in bacterial elimination, wound healing, and cancer treatments. Herein, we summarize the recently proposed strategies for achieving low-temperature PTT based on nanomaterials and introduce the synthesis, characteristics, and applications of these nanoplatforms. Additionally, the combination of PTT and other therapeutic modalities for defeating cancers and the synergistic cancer therapeutic effect of the combined treatments are discussed. Finally, the current limitations and future directions are proposed for inspiring more researchers to make contributions to promoting low-temperature PTT toward more successful preclinical and clinical disease treatments.

show abstract

“…终提高MPTT的疗效. Zhou等人 [25] 和Wang等人 [30] 将自噬抑制剂氯喹引入纳米材料中, 通过抑制肿瘤保护性自噬增强MPTT的肿瘤消融作用. Song等人 [32] 将PI3K 抑制剂LY294002装载入Bi 2 S 3 -Tween 20纳米材料中, HSP70的表达来激活BAX/BAK介导的线粒体途径细胞凋亡, 从而有效杀死大肠癌细胞.…”

Section: 温和光热疗法联合其他疗法unclassified