Inhibition of Bacterial Growth by Glucose in Media Devoid of Nicotinic Acid

TOXIC EFFECT OF GLUCOSE ON VIRULENT PASTEURELLA PESTIS IN CHEMICALLY DEFINED MEDIA

“…The literature records instances of the depression of bacterial growth by the inclusion of glucose in the medium. Glucose has usually been shown to exert its effect by interfering with amino acid metabolism (Kligler and Grossowicz, 1941;Kligler et al, 1943;Shaposhnikov and Tauson, 1948;Forbes and Sevag, 1951). Jordan (1952) believed the inhibition of growth of Pasteurella septica, when cultured with glucose in the medium, was due to the accumulation of inhibitory amounts of H202.…”

Relation of Bacteria to Vitamins and Other Growth Factors

“…However, most of the recent data in support of this view are general rather than specific in character. Kligler et al (148,149) reported that nicotinic acid and thiamine are required if glucose is present in the medium, but not if it is absent. Many papers have been published showing that thiamine (119,(321)(322)(323)358, 360, 365); riboflavin (2, 143), pantothenic acid (26, 78, 120), and nicotinic acid (or coenzyme I) (82, 211) increase respiration (oxygen uptake, carbon dioxide production or methylene blue reduction) by cells (e.g., Staphylococcus aureus, Propionibacterium pentosaceum, Proteus morganii, Lactobacillus mannitopoeus, dysentery bacilli, Hemophilus parainfluenzae) acting on various substrates (e.g., glucose, lactate, pyruvate).…”

The Mode of Action of the Sulfonamides

“…The 32 Experiments on the effects of nicotinic acid on sulfonamide action may be much more complicated than is at first apparent. Kligler et al (123) have recently found that, when nicotinic acid is lacking in a medium otherwise suitable for growth, the addition of glucose inhibits the growth of organisms (e.g., Proteus, dysentery bacilli, staphylococci) which are able to ferment this sugar in the presence of nicotinic acid. latter is a definite possibility, since there is evidence that pyridine-3-sulfonic acid inhibits this conversion (170).…”