Inhibition of calcium/calmodulin-dependent protein kinase in drosophila disrupts behavioral plasticity

Griffith, Leslie C.; Verselis, Lynne; Aitken, Kay Marie; Kyriacou, Charalambos P.; Danho, Waleed; Greenspan, Ralph J.

doi:10.1016/0896-6273(93)90337-q

Cited by 201 publications

(131 citation statements)

References 29 publications

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“…In some cases-as exemplified by the classic learning mutants in Drosophila (Greenspan, 1997;Sokolowski, 2001)-one may luck out and find hypomorphic alleles that are specifically associated with the behavioral role of the gene [e.g., merely a 10% reduction in the activity levels of CaMKII eliminates olfactory memory acquisition (Griffith et al, 1993)]. However, it is conceivable that in many cases it may be difficult if not impossible to affect the activity of a gene in such specific ways.…”

Section: What Comes Next? More Genetics More Genomics More Assays mentioning

confidence: 99%

Behavioral plasticity in C. elegans: Paradigms, circuits, genes

2002

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Life in the soil is an intellectual and practical challenge that the nematode Caenorhabditis elegans masters by utilizing 302 neurons. The nervous system assembled by these 302 neurons is capable of executing a variety of behaviors, some of respectable complexity. The simplicity of the nervous system, its thoroughly characterized structure, several sets of welldefined behaviors, and its genetic amenability combined with its isogenic background make C. elegans an attractive model organism to study the genetics of behavior. This review describes several behavioral plasticity paradigms in C. elegans and their underlying neuronal circuits and then goes on to review the forward genetic analysis that has been undertaken to identify genes involved in the execution of these behaviors. Lastly, the review outlines how reverse genetics and genomic approaches can guide the analysis of the role of genes in behavior and why and how they will complement the forward genetic analysis of behavior.

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Section: What Comes Next? More Genetics More Genomics More Assays mentioning

confidence: 99%

Behavioral plasticity in C. elegans: Paradigms, circuits, genes

2002

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“…Flies were cultured as described (9,10) at 25°C, 65% humidity, and 12-h light:12-h dark cycle. Unless insecticides were added to the culture, flies were raised on standard cornmeal, yeast, sucrose, glucose, and agar medium.…”

Section: Methodsmentioning

confidence: 99%

Drug-resistant Drosophila indicate glutamate-gated chloride channels are targets for the antiparasitics nodulisporic acid and ivermectin

Kane

Hirschberg

Qian

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

174

146

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The fruit fly Drosophila melanogaster was used to examine the mode of action of the novel insecticide and acaricide nodulisporic acid. Flies resistant to nodulisporic acid were selected by stepwise increasing the dose of drug in the culture media. The resistant strain, glc 1 , is at least 20-fold resistant to nodulisporic acid and 3-fold cross-resistant to the parasiticide ivermectin, and exhibited decreased brood size, decreased locomotion, and bang sensitivity. Binding assays using glc 1 head membranes showed a marked decrease in the affinity for nodulisporic acid and ivermectin. A combination of genetics and sequencing identified a proline to serine mutation (P299S) in the gene coding for the glutamategated chloride channel subunit DmGluCl␣. To examine the effect of this mutation on the biophysical properties of DmGluCl␣ channels, it was introduced into a recombinant DmGluCl␣, and RNA encoding wild-type and mutant subunits was injected into Xenopus oocytes. Nodulisporic acid directly activated wild-type and mutant DmGluCl␣ channels. However, mutant channels were Ϸ10-fold less sensitive to activation by nodulisporic acid, as well as ivermectin and the endogenous ligand glutamate, providing direct evidence that nodulisporic acid and ivermectin act on DmGluCl␣ channels.

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“…Furthermore, in D. melanogaster, these cycles were modulated in a predictable fashion by the circadian rhythm period (per) mutations (13): per L males with long 29-h circadian cycles also sang with long ∼80-s song cycles, whereas circadian arrhythmic per 01 males showed a corresponding song phenotype (2,4,9,10). These cycles were shown to have functional significance in playback experiments in which females were shown to be most responsive to both their species-specific IPI and cycle (2,(14)(15)(16).…”

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confidence: 99%

Failure to reproduce period -dependent song cycles in Drosophila is due to poor automated pulse-detection and low-intensity courtship

Kyriacou

Green

Piffer

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Stern has criticized a body of work from several groups that have independently studied the so-called "Kyriacou and Hall" courtship song rhythms of male Drosophila melanogaster, claiming that these ultradian ∼60-s cycles in the interpulse interval (IPI) are statistical artifacts that are not modulated by mutations at the period (per) locus [Stern DL (2014) BMC Biol 12:38]. We have scrutinized Stern's raw data and observe that his automated song pulse-detection method identifies only ∼50% of the IPIs found by manual (visual and acoustic) monitoring. This critical error is further compounded by Stern's use of recordings with very little song, the large majority of which do not meet the minimal song intensity criteria which Kyriacou and Hall used in their studies. Consequently most of Stern's recordings only contribute noise to the analyses. Of the data presented by Stern, only per L and a small fraction of wild-type males sing vigorously, so we limited our reanalyses to these genotypes. We manually reexamined Stern's raw song recordings and analyzed IPI rhythms using several independent time-series analyses. We observe that per L songs show significantly longer song periods than wildtype songs, with values for both genotypes close to those found in previous studies. These per-dependent differences disappear when the song data are randomized. We conclude that Stern's negative findings are artifacts of his inadequate pulse-detection methodology coupled to his use of low-intensity courtship song records.D uring courtship, the Drosophila melanogaster male vibrates his wing toward the female and produces a series of pulses and hums (1). The pulses have a variable interpulse interval (IPI), which ranges from 15 to 80 ms but usually averages between 30 and 40 ms, whereas sympatric Drosophila simulans mean IPIs vary from 45 to 80 ms depending on the strain (2, 3). In 1980, in these pages, the first of a series of studies by Kyriacou, Hall, and their collaborators revealed that superimposed on these IPIs was a low-amplitude oscillation of about 60 s in D. melanogaster and 40 s in D. simulans (4-12). Furthermore, in D. melanogaster, these cycles were modulated in a predictable fashion by the circadian rhythm period (per) mutations (13): per L males with long 29-h circadian cycles also sang with long ∼80-s song cycles, whereas circadian arrhythmic per 01 males showed a corresponding song phenotype (2, 4, 9, 10). These cycles were shown to have functional significance in playback experiments in which females were shown to be most responsive to both their species-specific IPI and cycle (2, 14-16).The work of Kyriacou, Hall, and collaborators was performed in the late 1970s and 1980s using extremely laborious analog technology, so to attain any kind of throughput, IPIs were binned into consecutive 10-s intervals and a mean IPI calculated on a minimum of 10 IPIs (2, 4-6, 8). Initially, sine/cosine functions were fitted to the mean IPIs (2, 4-6, 8), and this was later complemented by spectral analyses, with both types of statisti...

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Inhibition of calcium/calmodulin-dependent protein kinase in drosophila disrupts behavioral plasticity

Cited by 201 publications

References 29 publications

Behavioral plasticity in C. elegans: Paradigms, circuits, genes

Behavioral plasticity in C. elegans: Paradigms, circuits, genes

Drug-resistant Drosophila indicate glutamate-gated chloride channels are targets for the antiparasitics nodulisporic acid and ivermectin

Failure to reproduce period -dependent song cycles in Drosophila is due to poor automated pulse-detection and low-intensity courtship

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