Inhibition of Candida albicans biofilm formation on denture material

Redding, Spencer W.; Bhatt, Bakul; Rawls, H. Ralph; Siegel, Gregg; Scott, Kevin A.; Lopez-Ribot, José L.

doi:10.1016/j.tripleo.2009.01.021

Cited by 139 publications

(118 citation statements)

References 7 publications

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“…The choice of the imaging time course experiment was based upon the identification of architecturally early and mature biofilms in previous studies (3,11,24,33,55,56). Imaging of the denture demonstrated adherent yeast cells after 6 h (Fig.…”

Section: Denture Biofilm Imaging (I) Semmentioning

confidence: 99%

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Development and Validation of an In Vivo Candida albicans Biofilm Denture Model

Marchillo²,

et al. 2010

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The most common form of oral candidiasis, denture-associated stomatitis, involves biofilm growth on an oral prosthetic surface. Cells in this unique environment are equipped to withstand host defenses and survive antifungal therapy. Studies of the biofilm process on dentures have primarily been limited to in vitro models. We developed a rodent acrylic denture model and characterized the Candida albicans and mixed oral bacterial flora biofilm formation, architecture, and drug resistance in vivo, using time course quantitative culture experiments, confocal microscopy, scanning electron microscopy, and antifungal susceptibility assays. We also examined the utility of the model for measurement of C. albicans gene expression and tested the impact of a specific gene product (Bcr1p) on biofilm formation. Finally, we assessed the mucosal host response to the denture biofilm and found the mucosal histopathology to be consistent with that of acute human denture stomatitis, demonstrating fungal invasion and neutrophil infiltration. This current oral denture model mimics human denture stomatitis and should be useful for testing the impact of gene disruption on biofilm formation, studying the impact of anti-infectives, examining the biology of mixed Candida-oral bacterial flora biofilm infections, and characterizing the host immunologic response to this disease process.

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Section: Denture Biofilm Imaging (I) Semmentioning

confidence: 99%

“…In vitro biofilm models have the advantage of lower cost and are ideal for high-throughput screening assays and antifungal testing (12,56,61,71). These models have provided important phenotypic information regarding drug resistance in Candida biofilms.…”

mentioning

confidence: 99%

Development and Validation of an In Vivo Candida albicans Biofilm Denture Model

Marchillo²,

et al. 2010

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“…The active constituents of Geranium oil are "beta citronellol" and "geranoil". [4,5] Bacterial organisms: Streptococcus mutans is the main organism responsible for causation of dental caries. The mutability of this microorganism is responsible for development of resistance to any of the caries vaccine tested till date.…”

Section: Phytomedicine Derivatives and Their Target Organismsmentioning

confidence: 99%

Phytomedicines in Dentistry

M.D.S.¹

2014

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ABSTRACT:In this era of emergence of superbugs, one is in dire need of treatment modalities that treat a patient without hampering the microflora and immunity status. Traditional medicines like herbal by-products are currently being researched over and reintroduced in the commercial market as old wine in new bottles. This paper highlights the use of such plant products in dentistry, short-termed as "Phytomedicines".

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“…6,7 . Dessa forma, vêm sendo utilizadas modificações químicas da carga superficial das resinas como recurso para retardar ou prevenir o efeito da adesão leveduriforme 6,8,9 . Esta revisão sistematiza o atual panorama epidemiológico da EP associada ao uso de próteses dentárias, bem como as atuais e novas opções de combate ao biofilme fúngico especializado na adesão desse tipo de biomaterial.…”

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