Inhibition of Cell Cycle and Induction of Apoptosis y Ethanol Leaves Extract of Chrysanthemum cinerariifolium (Trev.) In T47D Breast Cancer Cells

Mutiah, Roihatul; Inayatin, Alfiyah Laily; Annisa, Rahmi; Indrawijaya, Yen Yen Ari; Listiyana, Anik

doi:10.14499/indonesianjpharm31iss1pp1

Cited by 6 publications

(4 citation statements)

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“…One type of cancer cell that is commonly used for cytotoxic tests is 747D breast cancer cells [20]. Cytotoxic testing on M3 fraction using seven concentration series of 10; 50; 100; 250; 500; The 1000 and 1500 ppm listed in Table 5, as well as as a positive control in the form of 5-fluorouracil made seven series of concentrations, namely 10; 50; 250; 500; 750 and 1000 ppm listed in Table 6.…”

Section: Cytotoxic Test Of the Mtt Methodsmentioning

confidence: 99%

Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47D Breast Cancer Cells

Sapar

Millenia

Aritonang

et al. 2023

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Characterization of secondary metabolites and cytotoxic testing of Haliclona sp. against T47D breast cancer cells were conducted in this study. The objective was to assess the cytotoxicity of T47D cancer cells and identify the functional groups involved. The research methods employed included maceration, partitioning, phytochemical testing, toxicity testing using the BSLT method, separation through flash column chromatography (FCC), cytotoxic testing using the MTT method, and characterization using FTIR. The partition results of methanol extract consist of n-hexane, ethyl acetate, and methanol-water fractions. The methanol extract demonstrated high toxicity, with an LC50 of 5.21 ppm. Among the fractions, the ethyl acetate fraction exhibited the highest toxicity compared to the n-hexane and methanol-water fractions, with LC50 values of 25.76 ppm, 42.71 ppm, and 55.26 ppm, respectively. Phytochemical testing of the ethyl acetate fraction yielded positive results for terpenoids, steroids, alkaloids, and phenolic compounds. The ethyl acetate fraction was further separated using flash column chromatography, resulting in ten combined fractions (M1-M10). The cytotoxicity tests of the M3 fraction against 747D breast cancer cells showed non-toxic effects, with an IC50 value of 1382.29 ppm. The FTIR analysis of the M3 fraction revealed the presence of functional groups such as O-H, =C-H, C-H aliphatic, C=O, and C=C, which is indicative of the presence of terpenoids, steroids, and esters.

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Section: Cytotoxic Test Of the Mtt Methodsmentioning

confidence: 99%

Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47D Breast Cancer Cells

Sapar

Millenia

Aritonang

et al. 2023

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“…Next, silica gel plates are sprayed with H2SO4 10% v/v, and heated to 105 °C for 5 min using a TLC plate heater. The stain outcome was subsequently examined using an UV visualizer with a wavelength of 366 nm [16].…”

Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

Antiviral Activity of Saussurea Lappa Ethanol Extract Against Sars-Cov-2: In Vitro Study

FITRIANINGSIH,

SANTOSANINGSIH,

DJAJALAKSANA

et al. 2024

Int J App Pharm

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Objective: The study aims to investigate the antiviral activity of S. lappa against Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) in vitro. Methods: The extracts are obtained by ultrasonic-assisted extraction (UAE) with a 96% ethanol solvent. Thin-layer chromatography (TLC) uses n-hexane: ethyl acetate and chloroform: methanol) as mobile phases. The staining outcome is subsequently examined using UV visualizers with a wavelength of 366 nm. To assess the antiviral activity of Vero E6 cells, extracts were employed at doses of 25, 50, 75, and 100 µg/ml, with remdesivir serving as the positive control. Supernatants were collected on days 1, 2, 3, and 6 for qRT-PCR testing with target genes E and ORF1ab. Time-addition experiments were conducted to determine how the extract works as antiviral. Protein expression was tested with Western blots with antibodies S and N SARS-CoV-2. Results: TLC identifies terpenoid chemicals present in the ethanol extract of S. lappa. The ethanol extract of S. lappa exhibited antiviral effects against SARS-CoV-2, with an inhibitory concentration 50 (IC50) of 40 µg/ml, a cytotoxic concentration 50 (CC50) of 131.4 µg/ml, and a selectivity index of 3.51. The extract can potentially impact the entry-post-entry phase of SARS-CoV-2 infection in Vero cells. The immunoblotting results demonstrated a reduction in the expression of S and N proteins in the treatment group compared to the negative control. Conclusion: S. lappa ethanol extract has antiviral activity against SARS-CoV-2 based on an in vitro study.

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“…The higher the levels ofcompounds orphenadrine in plants, the higher the anticancer potential (Listiyana, et al, 2019). Mechanism of orphenadrine in Chrysanthemum sp as an anticancer is to inhibit the work of cyclin dependent kinase (CDK) which is a cell cycle regulator through activating kinase, so that it can inhibit the formation of an active CDK-Cyclin complex (Mutiah et al, 2020).…”

Section: Anticancer Activitymentioning

confidence: 99%

Systematic Review: Phytochemical Content and Pharmacological Effects of Chrysanthemum sp.

Mutiah

Marsyah

Saputra

2021

planar

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Chrysanthemum sp. is a plant often used by the community as an ornamental plant, drink, and traditional medicine. This study aims to examine the plant Chrysanthemum sp. from the aspect of phytochemical and pharmacological activity. The review method used in this study is the PRISMA method. Sources of data obtained using databases electronic from PubMed and Google Scholar. This review shows that plants Chrysanthemum sp. have phytochemical and pharmacological potential. The compounds contained in theplant Chrysanthemum sp. are morphine, genistein, hydantoin, limonene, g-terpinene, a-pinene, a-terpenyl acetate, 4-terpenyl acetate, a-calacorene, a-cedrene, b-bourbobene, elemol, 2- hexenal, and orphenadrine. Chrysanthemum sp. plants have anticancer, antibacterial, antioxidant, antifungal, analgesic, anti-inflammatory, and anticonsulvan activities.

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Inhibition of Cell Cycle and Induction of Apoptosis y Ethanol Leaves Extract of Chrysanthemum cinerariifolium (Trev.) In T47D Breast Cancer Cells

Cited by 6 publications

References 0 publications

Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47D Breast Cancer Cells

Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47D Breast Cancer Cells

Antiviral Activity of Saussurea Lappa Ethanol Extract Against Sars-Cov-2: In Vitro Study

Systematic Review: Phytochemical Content and Pharmacological Effects of Chrysanthemum sp.

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