Inhibition of cell proliferation and glucose uptake in human laryngeal carcinoma cells by antisense oligonucleotides against glucose transporter‐1

Zhou, Shui‐Hong; Fan, Jun; Chen, Xiaoming; Cheng, Ke‐Jia; Wang, Shenqing

doi:10.1002/hed.21137

Cited by 43 publications

(54 citation statements)

References 28 publications

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“…HIF-1α and GLUT-1 are the intrinsic hypoxia markers that have been studied the most in various tumours (13)(14)(15)(16)(17)(18). Nevertheless, few studies have investigated the value of HIF-1α (22,23,28,29) or GLUT-1 expression (24,25) alone for the prediction of clinical outcome and survival rate of laryngeal carcinoma and, to the best of our knowledge, there is …”

Section: Discussionmentioning

confidence: 99%

“…In a previous study of GLUT-1 expression in laryngeal carcinoma, the expression of GLUT-1 mRNA and protein was increased in laryngeal carcinoma Hep-2 cells and an antisense oligonucleotide against GLUT-l mRNA reduced the expression of GLUT-1 mRNA and protein, thus inhibiting glucose uptake and cell growth in Hep-2 cells (25). Based on these results, it was suggested that GLUT-1 is a potential therapeutic target for strategies designed to inhibit the progression of laryngeal cancer (25).…”

mentioning

confidence: 89%

“…This study identified no correlation between GLUT-1 overexpression and clinical outcome parameters of laryngeal carcinoma. A small quantity of studies have investigated HIF-1α (22,23) or GLUT-1 expression alone (24,25) in laryngeal carcinoma. The present study investigated the corre-lation between HIF-1α and GLUT-1 expression with respect to various clinical and pathological features of laryngeal carcinoma.…”

Section: Introductionmentioning

confidence: 99%

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Expression and significance of hypoxia-inducible factor-1α and glucose transporter-1 in laryngeal carcinoma

Chen

Jianying

et al. 2012

Oncology Letters

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Abstract. Malignant cells show increased glucose uptake in vitro and in vivo. This process is considered to be mediated by glucose transporters (GLUTs). Hypoxia-inducible factor-1α (HIF-1α) may upregulate GLUT-1 expression. Little is known about the correlation between HIF-1α and GLUT-1 expression in laryngeal carcinoma. The current study investigated this correlation immunohistochemically, according to various clinical and pathological features, in 49 paraffin-embedded archival tissue blocks from patients with laryngeal squamous cell carcinoma. HIF-1α and GLUT-1 expression was detected in 63.3 (31/49) and 55.1% (27/49) of the tumour samples, respectively. HIF-1α expression was significantly correlated with lymph node classification (P= 0.018), recurrence (P= 0.02) and metastasis (P= 0.031). GLUT-1A expression was significantly associated with recurrence (P=0.02) and metastasis (P=0.01). Univariate analyses revealed that HIF-1α (χ 2 =8.2; P=0.004) and GLUT-1 expression (χ 2 =9.0; P=0.003) were significantly associated with a poorer survival rate. In a multivariate analysis, GLUT-1 expression (P=0.006) was a significant predictor of poor survival rate, as well as the primary tumour site, lymph node invasion and distant metastasis. Based on Spearman's analysis, GLUT-1 expression and phosphatidylinositol 3-kinase (PI3K) expression were significantly correlated (r=0.504; P=0.000). This is the first study to demonstrate a significant correlation between GLUT-1 and HIF-1α expression in laryngeal carcinoma and to show increased GLUT-1 expression as an independent survival rate predictor. These results suggest that GLUT-1 is a potential new therapeutic target for laryngeal carcinoma.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Expression and significance of hypoxia-inducible factor-1α and glucose transporter-1 in laryngeal carcinoma

Chen

Jianying

et al. 2012

Oncology Letters

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“…The human genes encoding these proteins are named GLUT-l to -5 and GLUT-7 to -13; GLUT-6 and -14 are now known to be pseudogenes. Of the 14 isoforms, GLUT-1 appears to be the most ubiquitously distributed (10,12,13). Increased GLUT-1 levels and glucose uptake correlate with increased cellular growth and proliferation (12,13).…”

Section: Introductionmentioning

confidence: 99%

“…Of the 14 isoforms, GLUT-1 appears to be the most ubiquitously distributed (10,12,13). Increased GLUT-1 levels and glucose uptake correlate with increased cellular growth and proliferation (12,13). This isoform is overexpressed in many human cancer cells, and its appearance is correlated with aggressive biological behavior (10,12).…”

Section: Introductionmentioning

confidence: 99%

Recurrent prognostic factors and expression of GLUT-1, PI3K and p-Akt in adenoid cystic carcinomas of the head and neck: Clinicopathological features and biomarkers of adenoid cystic carcinoma

et al. 2012

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Abstract. The purpose of this study was to explore the factors associated with the recurrence of adenoid cystic carcinomas (ACCs). We examined the recurrence values of clinicopathological variables and GLUT-1, p-Akt and PI3K expression in 42 patients with ACC. Of the 42 patients, 17 developed recurrence following initial surgery. The positive rates of GLUT-1, PI3K and p-Akt protein expression in ACC were 38.1, 38.1 and 50.0%, respectively. The expression of GLUT-1, p-Akt or PI3K protein in ACC was higher than that in inflammatory lesions or benign tumors. Our study demonstrated that T stage, a positive resection margin, perineural invasion, surgery without postoperative radiotherapy and the expression of GLUT-1, PI3K and p-Akt were factors predictive of recurrence by univariate analyses. In multivariate analyses, perineural invasion, a positive resection margin and p-Akt were significant predictors of recurrence. Initial surgery is very significant in the recurrence of ACC. Overexpression of GLUT-1, PI3K and p-Akt may also play a role in its development and recurrence.

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Hesperetin impairs glucose uptake and inhibits proliferation of breast cancer cells

Yang

Wolfram

Boom

et al. 2012

Cell Biochemistry & Function

104

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The flavanone hesperetin is known to decrease basal glucose uptake, although the inhibitory mechanism is largely unknown. Here, we used MDA-MB-231 breast cancer cells to investigate the molecular pathways affected by hesperetin. The results indicate that the suppression of glucose uptake is caused by the down-regulation of glucose transporter 1 (GLUT1). Hesperetin was also found to inhibit insulin-induced glucose uptake through impaired cell membrane translocation of glucose transporter 4 (GLUT4). In addition, the phosphorylation of the insulin receptor-beta subunit (IR-beta) and Akt was suppressed. Hesperetin also decreased cellular proliferation, which is likely due to the inhibition of glucose uptake. Cancer cells are highly dependent on glucose and hesperetin may, therefore, have potential application as an anticancer agent.

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Inhibition of cell proliferation and glucose uptake in human laryngeal carcinoma cells by antisense oligonucleotides against glucose transporter‐1

Abstract: Glut-1 AS decreases glucose uptake and inhibits the proliferation of Hep-2 cells.

Cited by 43 publications

References 28 publications

Expression and significance of hypoxia-inducible factor-1α and glucose transporter-1 in laryngeal carcinoma

Expression and significance of hypoxia-inducible factor-1α and glucose transporter-1 in laryngeal carcinoma

Recurrent prognostic factors and expression of GLUT-1, PI3K and p-Akt in adenoid cystic carcinomas of the head and neck: Clinicopathological features and biomarkers of adenoid cystic carcinoma

Hesperetin impairs glucose uptake and inhibits proliferation of breast cancer cells

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