Inhibition of cellulases by phenols

Ximenes, Eduardo; Kim, Youngmi; Mosier, Nathan S.; Dien, Bruce S.; Ladisch, Michael R.

doi:10.1016/j.enzmictec.2009.11.001

Cited by 425 publications

(302 citation statements)

References 48 publications

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“…Irreversible loss of cellulases is observed with the non-specific binding of lignin, which subsequently reduces the efficiency of hydrolysis (Gao et al 2014). Lignin-derived products, such as phenolic compounds, have inhibitory effects on cellulases and other enzymes used in cellulose hydrolysis (Ximenes et al 2010. Both xylose and xylo-oligomers directly inhibit cellulases, leading to lower yields of glucose .…”

Section: Introductionmentioning

confidence: 99%

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

Hu¹,

Zhu²,

Fang³

et al. 2016

BioResources

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With the decreased demand for pulp and paper worldwide, the reorganization of pulp and paper mills for cellulosic sugar production is possible. To maximize cellulosic sugar production from the wood pulp with minimum resources, the effects of pH, buffer system, temperature, enzyme loadings, pulp concentrations, and mixing modes on enzymatic hydrolysis were investigated, one factor at a time. Temperature played an important role in enzymatic hydrolysis. When the temperature was lower than 45 °C, the sugar production declined dramatically to almost half of the maximum value. Increasing enzyme dosage, increasing pulp concentration, and adding xylanase increased sugar production. The intermittent manual mixing mode generated higher concentrations of sugars and could be used for large-scale production. At pilot-scale, the diverted pulp for the pulping process was directly hydrolyzed without any treatment, and the residue after hydrolysis was hydrolyzed by adding fresh enzymes. This study provides insight on economically feasible enzymatic hydrolysis of wood pulp at large-scale cellulosic sugar production.

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Section: Introductionmentioning

confidence: 99%

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

Hu¹,

Zhu²,

Fang³

et al. 2016

BioResources

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“…Indeed, a biomass treatment by high temperature liquid water induces hydrolysis of hemicellulose in monomers including xylose which decomposes into furfural which is inhibitor for anaerobic digestion (Qi and Xiuyang, 2007). Ximenes et al have studied the effects on cellulases activity of phenols released during lignocellulosic biomass pretreatment and observed an inhibition induced by phenolic compounds (Ximenes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Enhancing solubilisation and methane production kinetic of switchgrass by microwave pretreatment

Jackowiak

Frigon

Ribeiro

et al. 2011

Bioresource Technology

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This study investigated the effects of microwave pretreatment of switchgrass in order to enhance its anaerobic digestibility. Response surface analysis was applied to screen the effects of temperature and time of microwave pretreatment on matter solubilisation. The composite design showed that only temperature had a significant effect on solubilisation level. Then the effects of the microwave pretreatment were correlated to the pretreatment temperature. The sCOD/tCOD ratio was equal to 9.4% at 90°C and increased until 13.8% at 180°C. The BMP assays of 42 days showed that microwave pretreatment induced no change on the ultimate volume of methane but had an interesting effect on the reaction kinetic. Indeed, the time required to reach 80% of ultimate volume CH 4 is reduced by 4.5 days at 150°C using the microwave pretreatment.

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“…Effect of enzymatic process on different bran varieties showed that compared to respective controls, increase in soluble polyphenols, total polyphenols and free radical scavenging activity were more in Jyothi, whereas bound polyphenols, flavonoids, g-oryzanol and total antioxidant activity were more in IR64. Lower enzyme effect in Jyothi compared to IR64 on extractability of complex bio-molecules may be due to the inhibitory potential of higher content of polyphenols present in it (Ximenes et al 2010).…”

Section: Overall Effect Of Enzymatic Processes In Rice Branmentioning

confidence: 99%

Enzymatic processing of pigmented and non pigmented rice bran on changes in oryzanol, polyphenols and antioxidant activity

Prabhu

Jayadeep

2015

J Food Sci Technol

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Bran from different rice varieties is a treasure of nutrients and nutraceuticals, and its use is limited due to the poor sensory and functional properties. Application of enzymes can alter the functional and phytochemical properties. So the effect of endo-xylanase, cellulase and their combination on microstructural, nutraceutical and antioxidant properties of pigmented (Jyothi) and non-pigmented (IR64) rice bran were investigated. Scanning electron micrograph revealed micro structural changes in fibre structures on processing. All the enzymatic processing methods resulted in an increase in the content of oryzanol, soluble, bound and total polyphenols, flavonoid and tannin. It also showed an increase in the bioactivity with re-

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Inhibition of cellulases by phenols

Cited by 425 publications

References 48 publications

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

Enhancing solubilisation and methane production kinetic of switchgrass by microwave pretreatment

Enzymatic processing of pigmented and non pigmented rice bran on changes in oryzanol, polyphenols and antioxidant activity

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