Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

Abstract

With the decreased demand for pulp and paper worldwide, the reorganization of pulp and paper mills for cellulosic sugar production is possible. To maximize cellulosic sugar production from the wood pulp with minimum resources, the effects of pH, buffer system, temperature, enzyme loadings, pulp concentrations, and mixing modes on enzymatic hydrolysis were investigated, one factor at a time. Temperature played an important role in enzymatic hydrolysis. When the temperature was lower than 45 °C, the sugar production declined dramatically to almost half of the maximum value. Increasing enzyme dosage, increasing pulp concentration, and adding xylanase increased sugar production. The intermittent manual mixing mode generated higher concentrations of sugars and could be used for large-scale production. At pilot-scale, the diverted pulp for the pulping process was directly hydrolyzed without any treatment, and the residue after hydrolysis was hydrolyzed by adding fresh enzymes. This study provides insight on economically feasible enzymatic hydrolysis of wood pulp at large-scale cellulosic sugar production.

Evaluation of lignocellulose degradation and ethanol production via dilute acid and alkali pretreatment of hybrid Pennisetum

“…6). The results were similar to other studies following the trend of glucose concentration during fermentation (Kuo et al 2014;Hu et al 2016). The concentration of ethanol was highest at 72 h, and it remained unchanged as the fermentation time further increased.…”